Volume 272, Number 52, Issue of December 26, 1997 pp. 32951-32955

Characterization and Localization of P₂ Receptors in Rat Submandibular Gland Acinar and Duct Cells

(Received for publication, September 8, 1997, and in revised form, October 21, 1997)

From the Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75235

[Ca²⁺]_i and the Cl current were measured in isolated submandibular gland acinar and duct cells to characterize and localize the purinergic receptors expressed in these cells. In both cell types 2-3-benzoylbenzoyl (Bz)-ATP and ATP increased [Ca²⁺]_i mainly by activation of Ca²⁺ influx. UTP had only minimal effect on [Ca²⁺]_i at concentrations between 0.1 and 1 mM. However, a whole cell current recording showed that all nucleotides effectively activated Cl currents. Inhibition of signal transduction through G proteins by guanyl-5--thiophosphate revealed that the effect of ATP on Cl current was mediated in part by activation of a G protein-coupled and in part by a G protein-independent receptor. BzATP activated exclusively the G protein-independent portion, whereas UTP activated only the G protein-dependent portion of the Cl current. Measurement of [Ca²⁺]_i in the microperfused duct showed that ATP stimulated a [Ca²⁺]_i increase when applied to the luminal or the basolateral sides. BzATP increased [Ca²⁺]_i only when applied to the luminal side, whereas UTP at 100 µM increased [Ca²⁺]_i only when applied to the basolateral side. The combined results suggest that duct and possibly acinar cells express P₂z receptors in the luminal and P₂u receptors in the basolateral membrane.

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