| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Volume 272, Number 52, Issue of December 26, 1997
pp. 33125-33131
(Received for publication, November 14, 1996, and in revised form, September 4, 1997)
From the Department of Biochemistry, School of Medicine and
Biomedical Sciences, State University of New York at Buffalo,
Buffalo, New York 14214
We report a mouse cDNA that encodes a
317-amino acid short-chain dehydrogenase which recognizes as substrates
9-cis-retinol, 11-cis-retinol,
5
cDNA Cloning and Characterization of a
cis-Retinol/3
-Hydroxysterol Short-chain
Dehydrogenase
-androstan-3,17
-diol, and 5-androstan-3-ol-17-one. This
cis-retinol/androgen dehydrogenase (CRAD) shares closest amino acid similarity with mouse retinol dehydrogenase isozymes types 1 and 2 (86 and 91%, respectively). Recombinant CRAD uses NAD+ as its preferred cofactor and exhibits cooperative
kinetics for cis-retinoids, but Michaelis-Menten kinetics
for 3-hydroxysterols. Unlike recombinant retinol dehydrogenase
isozymes, recombinant CRAD was inhibited by 4-methylpyrazole, was not
stimulated by ethanol, and did not require phosphatidylcholine for
optimal activity. CRAD mRNA was expressed intensely in kidney and
liver, in contrast to retinol dehydrogenase isozymes, which show strong
mRNA expression only in liver. CRAD mRNA expression was
widespread (relative abundance): kidney (100) > liver (92) > small
intestine (9) = heart (9) > retinal pigment epithelium and sclera
(4.5) > brain (2) > retina and vitreous (1.6) > spleen (0.7) > testis (0.6) > lung (0.4). CRAD may catalyze the first step in an
enzymatic pathway from 9-cis-retinol to generate the
retinoid X receptor ligand 9-cis-retinoic acid and/or may
regenerate dihydrotestosterone from its catabolite 5-androstan-3,17-diol. These data also illustrate the
multifunctional nature of short-chain dehydrogenases and provide a
potential mechanism for androgen-retinoid interactions.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Liden and U. Eriksson Understanding Retinol Metabolism: Structure and Function of Retinol Dehydrogenases J. Biol. Chem., May 12, 2006; 281(19): 13001 - 13004. [Full Text] [PDF]
|
|
|
|
|
|
T. S. Kim, A. Maeda, T. Maeda, C. Heinlein, N. Kedishvili, K. Palczewski, and P. S. Nelson Delayed Dark Adaptation in 11-cis-Retinol Dehydrogenase-deficient Mice: A ROLE OF RDH11 IN VISUAL PROCESSES IN VIVO J. Biol. Chem., March 11, 2005; 280(10): 8694 - 8704. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Fan, B. Rohrer, G. Moiseyev, J.-x. Ma, and R. K. Crouch Isorhodopsin rather than rhodopsin mediates rod function in RPE65 knock-out mice PNAS, November 11, 2003; 100(23): 13662 - 13667. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-S. Song, W. Chen, M. Zhang, and J. L. Napoli Identification of a Mouse Short-chain Dehydrogenase/Reductase Gene, Retinol Dehydrogenase-similar: FUNCTION OF NON-CATALYTIC AMINO ACID RESIDUES IN ENZYME ACTIVITY J. Biol. Chem., October 10, 2003; 278(41): 40079 - 40087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lin, M. Zhang, M. Abraham, S. M. Smith, and J. L. Napoli Mouse Retinal Dehydrogenase 4 (RALDH4), Molecular Cloning, Cellular Expression, and Activity in 9-cis-Retinoic Acid Biosynthesis in Intact Cells J. Biol. Chem., March 7, 2003; 278(11): 9856 - 9861. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Brodeur, I. Gagnon, S. Mader, and P. V. Bhat Cloning of monkey RALDH1 and characterization of retinoid metabolism in monkey kidney proximal tubule cells J. Lipid Res., February 1, 2003; 44(2): 303 - 313. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. Stulnig, K. R. Steffensen, H. Gao, M. Reimers, K. Dahlman-Wright, G. U. Schuster, and J.-A. Gustafsson Novel Roles of Liver X Receptors Exposed by Gene Expression Profiling in Liver and Adipose Tissue Mol. Pharmacol., December 1, 2002; 62(6): 1299 - 1305. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. N. Rexer and D. E. Ong A Novel Short-Chain Alcohol Dehydrogenase from Rats with Retinol Dehydrogenase Activity, Cyclically Expressed in Uterine Epithelium Biol Reprod, November 1, 2002; 67(5): 1555 - 1564. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Molotkov, X. Fan, L. Deltour, M. H. Foglio, S. Martras, J. Farres, X. Pares, and G. Duester Stimulation of retinoic acid production and growth by ubiquitously expressed alcohol dehydrogenase Adh3 PNAS, April 16, 2002; 99(8): 5337 - 5342. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Molotkov, L. Deltour, M. H. Foglio, A. E. Cuenca, and G. Duester Distinct Retinoid Metabolic Functions for Alcohol Dehydrogenase Genes Adh1 and Adh4 in Protection against Vitamin A Toxicity or Deficiency Revealed in Double Null Mutant Mice J. Biol. Chem., April 12, 2002; 277(16): 13804 - 13811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Shang, K. Lai, A. I. Packer, J. Paik, W. S. Blaner, M. de Morais Vieira, P. Gouras, and D. J. Wolgemuth Targeted disruption of the mouse cis-retinol dehydrogenase gene: visual and nonvisual functions J. Lipid Res., April 1, 2002; 43(4): 590 - 597. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yang and H. K. W. Fong Synthesis of the All-trans-retinal Chromophore of Retinal G Protein-coupled Receptor Opsin in Cultured Pigment Epithelial Cells J. Biol. Chem., January 25, 2002; 277(5): 3318 - 3324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zhang, W. Chen, S. M. Smith, and J. L. Napoli Molecular Characterization of a Mouse Short Chain Dehydrogenase/Reductase Active with All-trans-retinol in Intact Cells, mRDH1 J. Biol. Chem., November 16, 2001; 276(47): 44083 - 44090. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. G. G. Driessen, H. J. Winkens, K. Hoffmann, L. D. Kuhlmann, B. P. M. Janssen, A. H. M. Van Vugt, J. P. Van Hooser, B. E. Wieringa, A. F. Deutman, K. Palczewski, et al. Disruption of the 11-cis-Retinol Dehydrogenase Gene Leads to Accumulation of cis-Retinols and cis-Retinyl Esters Mol. Cell. Biol., June 15, 2000; 20(12): 4275 - 4287. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. O. Hardy, R.-S. Ge, J. F. Catterall, Y.-t. Hou, T. M. Penning, and M. P. Hardy Identification of the Oxidative 3{alpha}-Hydroxysteroid Dehydrogenase Activity of Rat Leydig Cells as Type II Retinol Dehydrogenase Endocrinology, May 1, 2000; 141(5): 1608 - 1617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. V. Gamble, E. Shang, R. P. Zott, J. R. Mertz, D. J. Wolgemuth, and W. S. Blaner Biochemical properties, tissue expression, and gene structure of a short chain dehydrogenase/ reductase able to catalyze cis-retinol oxidation J. Lipid Res., December 1, 1999; 40(12): 2279 - 2292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Su, M. Lin, and J. L. Napoli Complementary Deoxyribonucleic Acid Cloning and Enzymatic Characterization of a Novel 17{beta}/3{alpha}-Hydroxysteroid/Retinoid Short Chain Dehydrogenase/Reductase Endocrinology, November 1, 1999; 140(11): 5275 - 5284. [Abstract] [Full Text]
|
|
|
|
|
|
R.-S. Ge, D. O. Hardy, J. F. Catterall, and M. P. Hardy Opposing Changes in 3{alpha}-Hydroxysteroid Dehydrogenase Oxidative and Reductive Activities in Rat Leydig Cells during Pubertal Development Biol Reprod, April 1, 1999; 60(4): 855 - 860. [Abstract] [Full Text]
|
|
|
|
 |
|
 A Simon, A Romert, A. Gustafson, J. McCaffery, and U Eriksson Intracellular localization and membrane topology of 11-cis retinol dehydrogenase in the retinal pigment epithelium suggest a compartmentalized synthesis of 11-cis retinaldehyde J. Cell Sci., January 2, 1999; 112(4): 549 - 558. [Abstract] [PDF]
|
|
|
|
|
|
W. H. Gough, S. VanOoteghem, T. Sint, and N. Y. Kedishvili cDNA Cloning and Characterization of a New Human Microsomal NAD+-dependent Dehydrogenase that Oxidizes All-trans-retinol and 3alpha -Hydroxysteroids J. Biol. Chem., July 31, 1998; 273(31): 19778 - 19785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Su, X. Chai, B. Kahn, and J. L. Napoli cDNA Cloning, Tissue Distribution, and Substrate Characteristics of a cis-Retinol/3alpha -Hydroxysterol Short-chain Dehydrogenase Isozyme J. Biol. Chem., July 10, 1998; 273(28): 17910 - 17916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G.-F. Jang, J. K. McBee, A. M. Alekseev, F. Haeseleer, and K. Palczewski Stereoisomeric Specificity of the Retinoid Cycle in the Vertebrate Retina J. Biol. Chem., September 1, 2000; 275(36): 28128 - 28138. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Grimm, E. Maser, E. Mobus, G. Klebe, K. Reuter, and R. Ficner The Crystal Structure of 3alpha -Hydroxysteroid Dehydrogenase/Carbonyl Reductase from Comamonas testosteroni Shows a Novel Oligomerization Pattern within the Short Chain Dehydrogenase/Reductase Family J. Biol. Chem., December 22, 2000; 275(52): 41333 - 41339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lin and J. L. Napoli cDNA Cloning and Expression of a Human Aldehyde Dehydrogenase (ALDH) Active with 9-cis-Retinal and Identification of a Rat Ortholog, ALDH12 J. Biol. Chem., December 15, 2000; 275(51): 40106 - 40112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Tryggvason, A. Romert, and U. Eriksson Biosynthesis of 9-cis-Retinoic Acid in Vivo. THE ROLES OF DIFFERENT RETINOL DEHYDROGENASES AND A STRUCTURE-ACTIVITY ANALYSIS OF MICROSOMAL RETINOL DEHYDROGENASES J. Biol. Chem., May 25, 2001; 276(22): 19253 - 19258. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Soref, Y.-P. Di, L. Hayden, Y. H. Zhao, M. A. Satre, and R. Wu Characterization of a Novel Airway Epithelial Cell-specific Short Chain Alcohol Dehydrogenase/Reductase Gene Whose Expression Is Up-regulated by Retinoids and Is Involved in the Metabolism of Retinol J. Biol. Chem., June 22, 2001; 276(26): 24194 - 24202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. V. Chetyrkin, O. V. Belyaeva, W. H. Gough, and N. Y. Kedishvili Characterization of a Novel Type of Human Microsomal 3alpha -Hydroxysteroid Dehydrogenase. UNIQUE TISSUE DISTRIBUTION AND CATALYTIC PROPERTIES J. Biol. Chem., June 15, 2001; 276(25): 22278 - 22286. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
