Volume 272, Number 52, Issue of December 26, 1997 pp. 33132-33139

Diverse Effects of BCL3 Phosphorylation on Its Modulation of NF-B p52 Homodimer Binding to DNA

(Received for publication, August 12, 1997, and in revised form, October 17, 1997)

From the Departments of Pathology and of Radiation and Cellular Oncology, University of Chicago, Chicago, Illinois 60637

IB proteins control the subcellular localization and DNA binding activity of NF-B transcription factors. BCL3 is a nuclear IB that can inhibit or enhance the binding of NF-B p50 or p52 homodimers to consensus DNA-binding (B) sequences or form a B-binding complex with homodimers. To study BCL3 function, we have used gel shift analysis and tagged protein and tagged DNA coprecipitation analyses. Our results show that at intermediate ratios of BCL3 to p52 all observed phosphoforms of BCL3 are able to form a B-binding complex with p52 homodimers. At low BCL3/p52 ratios, BCL3 increases the rate of p52 homodimer binding to B sites in the presence of nonconsensus DNA and dissociates from the complex. At high BCL3/p52 ratios, BCL3 forms a higher order inhibitory complex with p52 homodimers. All of these effects depend on BCL3 phosphorylation and relative concentration. These results indicate that BCL3 phosphorylation may affect its regulation of NF-B-dependent transcription in vivo.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. Zhang, M. A. Warren, S. F. Shoemaker, and M. M. Ip NF{kappa}B1/p50 Is Not Required for Tumor Necrosis Factor-Stimulated Growth of Primary Mammary Epithelial Cells: Implications for NF{kappa}B2/p52 and RelB Endocrinology, January 1, 2007; 148(1): 268 - 278. [Abstract] [Full Text] [PDF]

				P. Y. Yeh, S.-H. Kuo, K.-H. Yeh, S.-E. Chuang, C.-H. Hsu, W. C. Chang, H.-I Lin, M. Gao, and A.-L. Cheng A Pathway for Tumor Necrosis Factor-{alpha}-induced Bcl10 Nuclear Translocation: Bcl10 IS UP-REGULATED BY NF-{kappa}B AND PHOSPHORYLATED BY Akt1 AND THEN COMPLEXES WITH Bcl3 TO ENTER THE NUCLEUS J. Biol. Chem., January 6, 2006; 281(1): 167 - 175. [Abstract] [Full Text] [PDF]

				J. Wessells, M. Baer, H. A. Young, E. Claudio, K. Brown, U. Siebenlist, and P. F. Johnson BCL-3 and NF-{kappa}B p50 Attenuate Lipopolysaccharide-induced Inflammatory Responses in Macrophages J. Biol. Chem., November 26, 2004; 279(48): 49995 - 50003. [Abstract] [Full Text] [PDF]

				P. W. Bates and S. Miyamoto Expanded Nuclear Roles for I{kappa}Bs Sci. Signal., October 12, 2004; 2004(254): pe48 - pe48. [Abstract] [Full Text] [PDF]

				S. Grundstrom, P. Anderson, P. Scheipers, and A. Sundstedt Bcl-3 and NF{kappa}B p50-p50 Homodimers Act as Transcriptional Repressors in Tolerant CD4+ T Cells J. Biol. Chem., February 27, 2004; 279(9): 8460 - 8468. [Abstract] [Full Text] [PDF]

				H. Pang, M. Bartlam, Q. Zeng, H. Miyatake, T. Hisano, K. Miki, L.-L. Wong, G. F. Gao, and Z. Rao Crystal Structure of Human Pirin: AN IRON-BINDING NUCLEAR PROTEIN AND TRANSCRIPTION COFACTOR J. Biol. Chem., January 9, 2004; 279(2): 1491 - 1498. [Abstract] [Full Text] [PDF]

				N. J. Thornburg, R. Pathmanathan, and N. Raab-Traub Activation of Nuclear Factor-{kappa}B p50 Homodimer/Bcl-3 Complexes in Nasopharyngeal Carcinoma Cancer Res., December 1, 2003; 63(23): 8293 - 8301. [Abstract] [Full Text] [PDF]

				S. Rocha, A. M. Martin, D. W. Meek, and N. D. Perkins p53 Represses Cyclin D1 Transcription through Down Regulation of Bcl-3 and Inducing Increased Association of the p52 NF-{kappa}B Subunit with Histone Deacetylase 1 Mol. Cell. Biol., July 1, 2003; 23(13): 4713 - 4727. [Abstract] [Full Text] [PDF]

				M. Witcher, D. T. Ross, C. Rousseau, L. Deluca, and W. H. Miller Jr Synergy between all-trans retinoic acid and tumor necrosis factor pathways in acute leukemia cells Blood, July 1, 2003; 102(1): 237 - 245. [Abstract] [Full Text] [PDF]

				S. Malek, D.-B. Huang, T. Huxford, S. Ghosh, and G. Ghosh X-ray Crystal Structure of an I{kappa}B{beta}{middle dot}NF-{kappa}B p65 Homodimer Complex J. Biol. Chem., June 13, 2003; 278(25): 23094 - 23100. [Abstract] [Full Text] [PDF]

				A. K. Moorthy and G. Ghosh p105{middle dot}Ikappa Bgamma and Prototypical Ikappa Bs Use a Similar Mechanism to Bind but a Different Mechanism to Regulate the Subcellular Localization of NF-kappa B J. Biol. Chem., January 3, 2003; 278(1): 556 - 566. [Abstract] [Full Text] [PDF]

				R. B. HUNTER, E. J. STEVENSON, A. KONCAREVIC, H. MITCHELL-FELTON, D. A. ESSIG, and S. C. KANDARIAN Activation of an alternative NF-{kappa}B pathway in skeletal muscle during disuse atrophy FASEB J, April 1, 2002; 16(6): 529 - 538. [Abstract] [Full Text] [PDF]

				D. Tsavachidou, W. Podrzucki, J. Seykora, and S. L. Berger Gene Array Analysis Reveals Changes in Peripheral Nervous System Gene Expression following Stimuli That Result in Reactivation of Latent Herpes Simplex Virus Type 1: Induction of Transcription Factor Bcl-3 J. Virol., October 15, 2001; 75(20): 9909 - 9917. [Abstract] [Full Text] [PDF]

				T. G. Willis and M. J. S. Dyer The role of immunoglobulin translocations in the pathogenesis of B-cell malignancies Blood, August 1, 2000; 96(3): 808 - 822. [Full Text] [PDF]

				X. Feng, Y. Jiang, P. Meltzer, and P. M. Yen Thyroid Hormone Regulation of Hepatic Genes in Vivo Detected by Complementary DNA Microarray Mol. Endocrinol., July 1, 2000; 14(7): 947 - 955. [Abstract] [Full Text]

				H. Haruta, A. Kato, and K. Todokoro Isolation of a Novel Interleukin-1-inducible Nuclear Protein Bearing Ankyrin-repeat Motifs J. Biol. Chem., April 13, 2001; 276(16): 12485 - 12488. [Abstract] [Full Text] [PDF]

				A. R. Brasier, M. Lu, T. Hai, Y. Lu, and I. Boldogh NF-kappa B-inducible BCL-3 Expression Is an Autoregulatory Loop Controlling Nuclear p50/NF-kappa B1 Residence J. Biol. Chem., August 17, 2001; 276(34): 32080 - 32093. [Abstract] [Full Text] [PDF]