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Volume 272, Number 52, Issue of December 26, 1997 pp. 33319-33326

Inhibition of the Iron-induced ZmFer1 Maize Ferritin Gene Expression by Antioxidants and Serine/Threonine Phosphatase Inhibitors

(Received for publication, April 7, 1997, and in revised form, September 10, 1997)

From the Laboratoire de Biochimie et Physiologie Moléculaire des Plantes, CNRS (Unité de Recherche 2133), Institut National de la Recherche Agronomique et École Nationale Supérieure d'Agronomie, F-34060 Montpellier cédex 1, France

Two pathways have been implicated in the regulation of maize ferritin synthesis in response to iron. One of them involves the plant hormone abscisic acid (ABA) and controls the expression of ZmFer2 gene(s). Another pathway, ABA-independent, has been characterized in a de-rooted maize plantlet system and involves an oxidative step. The ZmFer1 maize ferritin gene is not regulated by ABA, and it is shown in this paper that the corresponding mRNA accumulates in de-rooted maize plantlets and BMS (Black Mexican Sweet) maize cell suspension cultures in response to iron via the oxidative pathway described previously. To investigate ZmFer1 gene regulation further, the BMS cell system has been used to develop a transient expression assay using a ZmFer1--glucuronidase fusion. Both iron induction and antioxidant inhibition of ZmFer1 gene expression were observed in this system. Using Northern blot analysis and transient expression experiments, it was shown that both okadaic acid and calyculin A, two serine/ threonine phosphatase inhibitors, specifically inhibit ZmFer1 gene expression. These data indicate that an okadaic acid-sensitive protein phosphatase activity is involved in the regulation of the ZmFer1 ferritin gene in maize cells, and this activity is required for iron-induced expression of this gene.

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