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Volume 272, Number 6, Issue of February 7, 1997 pp. 3141-3144
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

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COMMUNICATION:
Identification of Previously Unrecognized Common Elements in Eukaryotic Promoters
A RIBOSOMAL RNA GENE INITIATOR ELEMENT FOR RNA POLYMERASE I

(Received for publication, November 20, 1996)

Catherine A. Radebaugh , Xiaoliang Gong , Blaine Bartholomew ^§ and Marvin R. Paule

From the  Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523-1870 and the ^§ Department of Medical Biochemistry, Southern Illinois University, Carbondale, Illinois 62901-4409

A new ribosomal RNA promoter element with a functional role similar to the RNA polymerase II initiator (Inr) was identified. This sequence, which we dub the ribosomal Inr (rInr) is unusually conserved, even in normally divergent RNA polymerase I promoters. It functions in the recruitment of the fundamental, TATA-binding protein (TBP)-containing transcription factor, TIF-IB. All upstream elements of the exceptionally strong Acanthamoeba castellanii ribosomal RNA core promoter, to within 6 base pairs of the transcription initiation site (tis), can be deleted without loss of specific transcription initiation. Thus, the A. castellanii promoter can function in a manner similar to RNA polymerase II TATA-less promoters. Sequence-specific photo-cross-linking localizes a 96-kDa subunit of TIF-IB and the second largest RNA polymerase I subunit (A¹³³) to the rInr sequence. A¹⁸⁵ also photo-cross-links when polymerase is stalled at +7.

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