| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, August 22, 1996, and in revised form, October 23, 1996)
From the Dipartimento di Biologia e Patologia Cellulare e
Molecolare "L. Califano" and Centro di Endocrinologia ed Oncologia
Sperimentale del Consiglio Nazionale delle Ricerche and
§ Dipartimento di Neuroscienze e della Comunicazone
Interumana, Università di Napoli "Federico II," Via S. Pansini 5, 80131, Napoli, Italy and ¶ Dipartimento di Medicina
Sperimentale e Clinica, Università di Reggio Calabria,
88100 Catanzaro, Italy
Rab7 is a small GTPase localized to the late
endosomal compartment. Its function was investigated by overexpressing
dominant negative or constitutively active mutants in BHK-21 cells. The effects of such overexpression on the internalization and/or
degradation of different endocytic markers and on the morphology of the
late endosomal compartment were analyzed. We observed a marked
inhibition of the degradation of 125I-low density
lipoproteins in cells transfected with the Rab7 dominant negative
mutants while the rate of internalization was not affected. Moreover in
these cells there was an accumulation of many small vesicles scattered
throughout the cytoplasm. In contrast, overexpression of the activating
mutants led to the appearance of atypically large endocytic structures
and caused a dramatic change in the distribution of the
cation-independent mannose 6-phosphate receptor. Our data indicate that
the Rab7 protein in mammalian cells is present on a late endosomal
compartment much larger than the compartment labeled by the
cation-independent mannose 6-phosphate receptor. Rab7 also appears to
play a fundamental role in controlling late endocytic membrane
traffic.
Volume 272, Number 7,
Issue of February 14, 1997
pp. 4391-4397
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. R. Spinosa, C. Progida, A. De Luca, A. M. R. Colucci, P. Alifano, and C. Bucci Functional Characterization of Rab7 Mutant Proteins Associated with Charcot-Marie-Tooth Type 2B Disease J. Neurosci., February 13, 2008; 28(7): 1640 - 1648. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. H. Shah, N. L. Cianciola, J. L. Mills, F. D. Sonnichsen, and C. Carlin Adenovirus RID{alpha} regulates endosome maturation by mimicking GTP-Rab7 J. Cell Biol., December 3, 2007; 179(5): 965 - 980. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Brumell and M. A. Scidmore Manipulation of Rab GTPase Function by Intracellular Bacterial Pathogens Microbiol. Mol. Biol. Rev., December 1, 2007; 71(4): 636 - 652. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Sun, A.-E. Deghmane, H. Soualhine, T. Hong, C. Bucci, A. Solodkin, and Z. Hmama Mycobacterium bovis BCG disrupts the interaction of Rab7 with RILP contributing to inhibition of phagosome maturation J. Leukoc. Biol., December 1, 2007; 82(6): 1437 - 1445. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. T. Lee, C. Y. Y. Szeto, T. P. Ng, and H. S. Kwan Endocytosis in the Shiitake Mushroom Lentinula edodes and Involvement of GTPase LeRAB7 Eukaryot. Cell, December 1, 2007; 6(12): 2406 - 2418. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. V. Samuelson, C. E. Carr, and G. Ruvkun Gene activities that mediate increased life span of C. elegans insulin-like signaling mutants Genes & Dev., November 15, 2007; 21(22): 2976 - 2994. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. W. Wong, S. J. Scales, and D. E. Reilly DNA Internalized via Caveolae Requires Microtubule-dependent, Rab7-independent Transport to the Late Endocytic Pathway for Delivery to the Nucleus J. Biol. Chem., August 3, 2007; 282(31): 22953 - 22963. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Rodriguez, G. Torrent, M. Bosch, F. Rayne, J.-F. Dubremetz, M. Ribo, A. Benito, M. Vilanova, and B. Beaumelle Intracellular pathway of Onconase that enables its delivery to the cytosol J. Cell Sci., April 15, 2007; 120(8): 1405 - 1411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Walthall, M. Moses, and J. I. Horabin A large complex containing Patched and Smoothened initiates Hedgehog signaling in Drosophila J. Cell Sci., March 1, 2007; 120(5): 826 - 837. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Meertens, C. Bertaux, and T. Dragic Hepatitis C Virus Entry Requires a Critical Postinternalization Step and Delivery to Early Endosomes via Clathrin-Coated Vesicles J. Virol., December 1, 2006; 80(23): 11571 - 11578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Cooper and Y. Shaul Clathrin-mediated Endocytosis and Lysosomal Cleavage of Hepatitis B Virus Capsid-like Core Particles J. Biol. Chem., June 16, 2006; 281(24): 16563 - 16569. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sabbatini, M. Santillo, A. Pisani, R. Paterno, F. Uccello, R. Seru, G. Matrone, G. Spagnuolo, M. Andreucci, V. Serio, et al. Inhibition of Ras/ERK1/2 signaling protects against postischemic renal injury Am J Physiol Renal Physiol, June 1, 2006; 290(6): F1408 - F1415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. P. Ceresa and S. J. Bahr rab7 Activity Affects Epidermal Growth Factor:Epidermal Growth Factor Receptor Degradation by Regulating Endocytic Trafficking from the Late Endosome J. Biol. Chem., January 13, 2006; 281(2): 1099 - 1106. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Saxena, C. Bucci, J. Weis, and A. Kruttgen The Small GTPase Rab7 Controls the Endosomal Trafficking and Neuritogenic Signaling of the Nerve Growth Factor Receptor TrkA J. Neurosci., November 23, 2005; 25(47): 10930 - 10940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Sun, K. G. Buki, O. Ettala, J. P. Vaaraniemi, and H. K. Vaananen Possible Role of Direct Rac1-Rab7 Interaction in Ruffled Border Formation of Osteoclasts J. Biol. Chem., September 16, 2005; 280(37): 32356 - 32361. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Holtta-Vuori, F. Alpy, K. Tanhuanpaa, E. Jokitalo, A.-L. Mutka, and E. Ikonen MLN64 Is Involved in Actin-mediated Dynamics of Late Endocytic Organelles Mol. Biol. Cell, August 1, 2005; 16(8): 3873 - 3886. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. G. Ganley, K. Carroll, L. Bittova, and S. Pfeffer Rab9 GTPase Regulates Late Endosome Size and Requires Effector Interaction for Its Stability Mol. Biol. Cell, December 1, 2004; 15(12): 5420 - 5430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jager, C. Bucci, I. Tanida, T. Ueno, E. Kominami, P. Saftig, and E.-L. Eskelinen Role for Rab7 in maturation of late autophagic vacuoles J. Cell Sci., September 15, 2004; 117(20): 4837 - 4848. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. B. Scott, I. Michailidis, Y. Mu, D. Logothetis, and M. D. Ehlers Endocytosis and Degradative Sorting of NMDA Receptors by Conserved Membrane-Proximal Signals J. Neurosci., August 11, 2004; 24(32): 7096 - 7109. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Marsman, I. Jordens, C. Kuijl, L. Janssen, and J. Neefjes Dynein-mediated Vesicle Transport Controls Intracellular Salmonella Replication Mol. Biol. Cell, June 1, 2004; 15(6): 2954 - 2964. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Junutula, A. M. De Maziere, A. A. Peden, K. E. Ervin, R. J. Advani, S. M. van Dijk, J. Klumperman, and R. H. Scheller Rab14 Is Involved in Membrane Trafficking between the Golgi Complex and Endosomes Mol. Biol. Cell, May 1, 2004; 15(5): 2218 - 2229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Vendeville, F. Rayne, A. Bonhoure, N. Bettache, P. Montcourrier, and B. Beaumelle HIV-1 Tat Enters T Cells Using Coated Pits before Translocating from Acidified Endosomes and Eliciting Biological Responses Mol. Biol. Cell, May 1, 2004; 15(5): 2347 - 2360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Hmama, K. Sendide, A. Talal, R. Garcia, K. Dobos, and N. E. Reiner Quantitative analysis of phagolysosome fusion in intact cells: inhibition by mycobacterial lipoarabinomannan and rescue by an 1{alpha},25-dihydroxyvitamin D3-phosphoinositide 3-kinase pathway J. Cell Sci., April 15, 2004; 117(10): 2131 - 2140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Guignot, E. Caron, C. Beuzon, C. Bucci, J. Kagan, C. Roy, and D. W. Holden Microtubule motors control membrane dynamics of Salmonella-containing vacuoles J. Cell Sci., March 1, 2004; 117(7): 1033 - 1045. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ono and E. O. Freed Cell-Type-Dependent Targeting of Human Immunodeficiency Virus Type 1 Assembly to the Plasma Membrane and the Multivesicular Body J. Virol., February 1, 2004; 78(3): 1552 - 1563. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Henry, A. D. Hoppe, N. Joshi, and J. A. Swanson The uniformity of phagosome maturation in macrophages J. Cell Biol., January 19, 2004; 164(2): 185 - 194. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Mazel, Y. Leshem, B. S. Tiwari, and A. Levine Induction of Salt and Osmotic Stress Tolerance by Overexpression of an Intracellular Vesicle Trafficking Protein AtRab7 (AtRabG3e) Plant Physiology, January 1, 2004; 134(1): 118 - 128. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Tiwari, J. E. Italiano Jr, D. C. Barral, E. H. Mules, E. K. Novak, R. T. Swank, M. C. Seabra, and R. A. Shivdasani A role for Rab27b in NF-E2-dependent pathways of platelet formation Blood, December 1, 2003; 102(12): 3970 - 3979. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Dupre, Z. Chen, C. Le Gouill, C. Theriault, J.-L. Parent, M. Rola-Pleszczynski, and J. Stankova Trafficking, Ubiquitination, and Down-regulation of the Human Platelet-activating Factor Receptor J. Biol. Chem., November 28, 2003; 278(48): 48228 - 48235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Mizuno, A. Kitamura, and T. Sasaki Rabring7, a Novel Rab7 Target Protein with a RING Finger Motif Mol. Biol. Cell, September 1, 2003; 14(9): 3741 - 3752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. A. Kelley and J. S. Schorey Mycobacterium's Arrest of Phagosome Maturation in Macrophages Requires Rab5 Activity and Accessibility to Iron Mol. Biol. Cell, August 1, 2003; 14(8): 3366 - 3377. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. I. Christensen, O. Devuyst, G. Dom, R. Nielsen, P. Van Der Smissen, P. Verroust, M. Leruth, W. B. Guggino, and P. J. Courtoy Loss of chloride channel ClC-5 impairs endocytosis by defective trafficking of megalin and cubilin in kidney proximal tubules PNAS, July 8, 2003; 100(14): 8472 - 8477. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Zhu, L. Zheng, K. Suyama, and M. P. Scott Altered localization of Drosophila Smoothened protein activates Hedgehog signal transduction Genes & Dev., May 15, 2003; 17(10): 1240 - 1252. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G.-H. Fan, L. A. Lapierre, J. R. Goldenring, and A. Richmond Differential regulation of CXCR2 trafficking by Rab GTPases Blood, March 15, 2003; 101(6): 2115 - 2124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Duclos, R. Corsini, and M. Desjardins Remodeling of endosomes during lysosome biogenesis involves `kiss and run' fusion events regulated by rab5 J. Cell Sci., March 1, 2003; 116(5): 907 - 918. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zheng, R. Ravatn, Y. Lin, W.-C. Shih, A. Rabson, R. Strair, E. Huberman, A. Conney, and K.-V. Chin Gene expression of TPA induced differentiation in HL-60 cells by DNA microarray analysis Nucleic Acids Res., October 15, 2002; 30(20): 4489 - 4499. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Beron, M. G. Gutierrez, M. Rabinovitch, and M. I. Colombo Coxiella burnetii Localizes in a Rab7-Labeled Compartment with Autophagic Characteristics Infect. Immun., October 1, 2002; 70(10): 5816 - 5821. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Croizet-Berger, C. Daumerie, M. Couvreur, P. J. Courtoy, and M.-F. van den Hove The endocytic catalysts, Rab5a and Rab7, are tandem regulators of thyroid hormone production PNAS, June 11, 2002; 99(12): 8277 - 8282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Lindsay, A. G. Hendrick, G. Cantalupo, F. Senic-Matuglia, B. Goud, C. Bucci, and M. W. McCaffrey Rab Coupling Protein (RCP), a Novel Rab4 and Rab11 Effector Protein J. Biol. Chem., March 29, 2002; 277(14): 12190 - 12199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Rosenfeld, R. H. Moore, K.-P. Zimmer, E. Alpizar-Foster, W. Dai, M. N. Zarka, and B. J. Knoll Lysosome proteins are redistributed during expression of a GTP-hydrolysis-defective rab5a J. Cell Sci., March 14, 2002; 114(24): 4499 - 4508. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Mesa, C. Salomon, M. Roggero, P. D. Stahl, and L. S. Mayorga Rab22a affects the morphology and function of the endocytic pathway J. Cell Sci., March 13, 2002; 114(22): 4041 - 4049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Fratti, J. M. Backer, J. Gruenberg, S. Corvera, and V. Deretic Role of phosphatidylinositol 3-kinase and Rab5 effectors in phagosomal biogenesis and mycobacterial phagosome maturation arrest J. Cell Biol., August 6, 2001; 154(3): 631 - 644. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Fiorentini, L. Falzano, A. Fabbri, A. Stringaro, M. Logozzi, S. Travaglione, S. Contamin, G. Arancia, W. Malorni, and S. Fais Activation of Rho GTPases by Cytotoxic Necrotizing Factor 1 Induces Macropinocytosis and Scavenging Activity in Epithelial Cells Mol. Biol. Cell, July 1, 2001; 12(7): 2061 - 2073. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Kujala, A. Ikäheimonen, N. Ehsani, H. Vihinen, P. Auvinen, and L. Kääriäinen Biogenesis of the Semliki Forest Virus RNA Replication Complex J. Virol., April 15, 2001; 75(8): 3873 - 3884. [Abstract] [Full Text]
|
|
|
|
|
|
A. Rupper, B. Grove, and J. Cardelli Rab7 regulates phagosome maturation in Dictyostelium J. Cell Sci., January 7, 2001; 114(13): 2449 - 2460. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-G. Li, J. L. Benovic, and L.-Y. Liu-Chen Mechanisms of Agonist-Induced Down-Regulation of the Human kappa -Opioid Receptor: Internalization Is Required for Down-Regulation Mol. Pharmacol., October 1, 2000; 58(4): 795 - 801. [Abstract] [Full Text]
|
|
|
|
|
|
C. Bucci, P. Thomsen, P. Nicoziani, J. McCarthy, and B. van Deurs Rab7: A Key to Lysosome Biogenesis Mol. Biol. Cell, February 1, 2000; 11(2): 467 - 480. [Abstract] [Full Text]
|
|
|
|
|
|
S Duclos, R Diez, J Garin, B Papadopoulou, A Descoteaux, H Stenmark, and M Desjardins Rab5 regulates the kiss and run fusion between phagosomes and endosomes and the acquisition of phagosome leishmanicidal properties in RAW 264.7 macrophages J. Cell Sci., January 10, 2000; 113(19): 3531 - 3541. [Abstract] [PDF]
|
|
|
|
|
|
M Ohashi, I Miwako, A Yamamoto, and K Nagayama Arrested maturing multivesicular endosomes observed in a Chinese hamster ovary cell mutant, LEX2, isolated by repeated flow-cytometric cell sorting J. Cell Sci., January 6, 2000; 113(12): 2187 - 2205. [Abstract] [PDF]
|
|
|
|
|
|
J Somsel Rodman and A Wandinger-Ness Rab GTPases coordinate endocytosis J. Cell Sci., January 1, 2000; 113(2): 183 - 192. [Abstract] [PDF]
|
|
|
|
|
|
P. A. Zuk and L. A. Elferink Rab15 Mediates an Early Endocytic Event in Chinese Hamster Ovary Cells J. Biol. Chem., August 6, 1999; 274(32): 22303 - 22312. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Hrboticky, G. Draude, G. Hapfelmeier, R. Lorenz, and P. C. Weber Lovastatin Decreases the Receptor-Mediated Degradation of Acetylated and Oxidized LDLs in Human Blood Monocytes During the Early Stage of Differentiation Into Macrophages Arterioscler. Thromb. Vasc. Biol., May 1, 1999; 19(5): 1267 - 1275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Press, Y. Feng, B. Hoflack, and A. Wandinger-Ness Mutant Rab7 Causes the Accumulation of Cathepsin D and Cation-independent Mannose 6-Phosphate Receptor in an Early Endocytic Compartment J. Cell Biol., March 9, 1998; 140(5): 1075 - 1089. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Mukhopadhyay, K. Funato, and P. D. Stahl Rab7 Regulates Transport from Early to Late Endocytic Compartments in Xenopus Oocytes J. Biol. Chem., May 16, 1997; 272(20): 13055 - 13059. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
