JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nelson, D. A.
Right arrow Articles by Ludlow, J. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nelson, D. A.
Right arrow Articles by Ludlow, J. W.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Volume 272, Number 7, Issue of February 14, 1997 pp. 4528-4535
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

High Molecular Weight Protein Phosphatase Type 1 Dephosphorylates the Retinoblastoma Protein

(Received for publication, October 1, 1996, and in revised form, November 18, 1996)

Deirdre A. Nelson Dagger , Nancy A. Krucher and John W. Ludlow Dagger

From the Dagger  Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry and the  Division of Developmental Therapeutics, University of Rochester Cancer Center, Rochester, New York 14642

pRb controls cell proliferation by restricting inappropriate entry of cells into the cell division cycle. As dephosphorylation of pRb during mitotic exit activates its growth suppressive function, identification of the protein phosphatase that dephosphorylates pRb, and characterization of the mechanism of its regulation, are essential to elucidating the mechanisms of cell growth control. By fractionating mitotic CV-1P cell extracts, we identify the protein phosphatase which dephosphorylates pRb as a type 1 serine/threonine phosphoprotein phosphatase (PP1). Molecular sizing analyses indicate that the catalytic enzyme (PP1c) is present in a high molecular weight complex, with a predicted molecular mass of 166 kDa. PP1-interacting proteins in the mitotic cell extracts are identified. Two PP1-interacting proteins (41 and 110 kDa) are shown to form distinct complexes with PP1c from fractions of separated mitotic cell extracts containing phosphorylase phosphatase activity. However, only the 110-kDa PP1-interacting protein is present in fractions containing pRb-directed phosphatase activity, identifying this protein as a putative activator of PP1 function toward pRb during mitosis.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 CarcinogenesisHome page
M. E. Castro, I. Ferrer, A. Cascon, M. V. Guijarro, M. Lleonart, S. R. y Cajal, J. F.M. Leal, M. Robledo, and A. Carnero
PPP1CA contributes to the senescence program induced by oncogenic Ras
Carcinogenesis, March 1, 2008; 29(3): 491 - 499.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. R. Gunawardena, B. L. Ruis, J. A. Meyer, M. Kapoor, and K. F. Conklin
NOM1 Targets Protein Phosphatase I to the Nucleolus
J. Biol. Chem., January 4, 2008; 283(1): 398 - 404.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
A. Martirosyan, S. Leonard, X. Shi, B. Griffith, P. Gannett, and J. Strobl
Actions of a Histone Deacetylase Inhibitor NSC3852 (5-Nitroso-8-quinolinol) Link Reactive Oxygen Species to Cell Differentiation and Apoptosis in MCF-7 Human Mammary Tumor Cells
J. Pharmacol. Exp. Ther., May 1, 2006; 317(2): 546 - 552.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
H. CEULEMANS and M. BOLLEN
Functional Diversity of Protein Phosphatase-1, a Cellular Economizer and Reset Button
Physiol Rev, January 1, 2004; 84(1): 1 - 39.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Y. Lin and J. A. DeCaprio
SV40 Large T Antigen Promotes Dephosphorylation of p130
J. Biol. Chem., November 21, 2003; 278(47): 46482 - 46487.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y.-M. Kim, T. Watanabe, P. B. Allen, Y.-M. Kim, S.-J. Lee, P. Greengard, A. C. Nairn, and Y.-G. Kwon
PNUTS, a Protein Phosphatase 1 (PP1) Nuclear Targeting Subunit. CHARACTERIZATION OF ITS PP1- AND RNA-BINDING DOMAINS AND REGULATION BY PHOSPHORYLATION
J. Biol. Chem., April 11, 2003; 278(16): 13819 - 13828.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
L. Zhao, T. Samuels, S. Winckler, C. Korgaonkar, V. Tompkins, M. C. Horne, and D. E. Quelle
Cyclin G1 Has Growth Inhibitory Activity Linked to the ARF-Mdm2-p53 and pRb Tumor Suppressor Pathways
Mol. Cancer Res., January 1, 2003; 1(3): 195 - 206.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. Broceno, S. Wilkie, and S. Mittnacht
RB activation defect in tumor cell lines
PNAS, October 29, 2002; 99(22): 14200 - 14205.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
H. Morimoto, H. Okamura, and T. Haneji
Interaction of Protein Phosphatase 1 Delta with Nucleolin in Human Osteoblastic Cells
J. Histochem. Cytochem., September 1, 2002; 50(9): 1187 - 1193.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Yan and M. C. Mumby
Distinct Roles for PP1 and PP2A in Phosphorylation of the Retinoblastoma Protein. PP2A REGULATES THE ACTIVITIES OF G1 CYCLIN-DEPENDENT KINASES
J. Biol. Chem., November 5, 1999; 274(45): 31917 - 31924.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
G. J. Milczarek, W. Chen, A. Gupta, J. D. Martinez, and G.T. Bowden
Okadaic acid mediates p53 hyperphosphorylation and growth arrest in cells with wild-type p53 but increases aberrant mitoses in cells with non-functional p53
Carcinogenesis, June 1, 1999; 20(6): 1043 - 1048.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. M. Sterner, S. Dew-Knight, C. Musahl, S. Kornbluth, and J. M. Horowitz
Negative Regulation of DNA Replication by the Retinoblastoma Protein Is Mediated by Its Association with MCM7
Mol. Cell. Biol., May 1, 1998; 18(5): 2748 - 2757.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
P. B. Allen, Y.-G. Kwon, A. C. Nairn, and P. Greengard
Isolation and Characterization of PNUTS, a Putative Protein Phosphatase 1 Nuclear Targeting Subunit
J. Biol. Chem., February 13, 1998; 273(7): 4089 - 4095.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Vulsteke, M. Beullens, E. Waelkens, W. Stalmans, and M. Bollen
Properties and Phosphorylation Sites of Baculovirus-expressed Nuclear Inhibitor of Protein Phosphatase-1 (NIPP-1)
J. Biol. Chem., December 26, 1997; 272(52): 32972 - 32978.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Kakinoki, J. Somers, and D. L. Brautigan
Multisite Phosphorylation and the Nuclear Localization of Phosphatase Inhibitor 2-Green Fluorescent Protein Fusion Protein during S Phase of the Cell Growth Cycle
J. Biol. Chem., December 19, 1997; 272(51): 32308 - 32314.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Ragolia, Q. Zuo, and N. Begum
Inhibition of Myogenesis by Depletion of the Glycogen-associated Regulatory Subunit of Protein Phosphatase-1 in Rat Skeletal Muscle Cells
J. Biol. Chem., August 18, 2000; 275(34): 26102 - 26108.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Vivo, R. A. Calogero, F. Sansone, V. Calabro, T. Parisi, L. Borrelli, S. Saviozzi, and G. La Mantia
The Human Tumor Suppressor ARF Interacts with Spinophilin/Neurabin II, a Type 1 Protein-phosphatase-binding Protein
J. Biol. Chem., April 20, 2001; 276(17): 14161 - 14169.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1997 by the American Society for Biochemistry and Molecular Biology.