| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, May 25, 1996, and in revised form, November 14, 1996)
From the INSERM U.311, The aim of our study was to evaluate the effect
of ADP and the role of cytoskeleton reorganization during reversible
and irreversible platelet aggregation induced by ADP and thrombin,
respectively, on the heterodimeric (p85
-p110) phosphoinositide
3-kinase translocation to the cytoskeleton and its activation.
Reversible ADP-induced aggregation was accompanied by a reversible
reorganization of the cytoskeleton and an increase in levels of the
regulatory subunit p85 in this cytoskeleton similar to the increase
observed in thrombin-activated platelets. This translocation followed a
course parallel to the amplitude of aggregation. No increase in levels of both phosphatidylinositol (3,4)-bisphosphate
(PtdIns(3,4)P2) and
phosphatidylinositol-(3,4,5)P3 could, however, be detected even at the maximum aggregation and PI 3-kinase translocation. Moreover, in contrast to the situation for thrombin stimulation, the
GTP-binding protein RhoA was hardly translocated to the cytoskeleton when platelets were stimulated with ADP, whereas translocation of
pp60c-src and focal adhesion kinase did occur.
These results suggest (i) translocation of signaling enzymes does not
necessarily imply their activation, (ii) the reversibility of
ADP-induced platelet aggregation may be the cause or the result of a
lack of PI 3-kinase activation and hence of PtdIns(3,4)P2
production, and (iii) RhoA does not seem to be involved in the ADP
activation pathway of platelets. Whether PtdIns(3,4)P2 or
RhoA may contribute to the stabilization of platelet aggregates remains
to be established.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M.-P. Gratacap, J.-P. Herault, C. Viala, A. Ragab, P. Savi, J.-M. Herbert, H. Chap, M. Plantavid, and B. Payrastre Fcgamma RIIA requires a Gi-dependent pathway for an efficient stimulation of phosphoinositide 3-kinase, calcium mobilization, and platelet aggregation Blood, November 15, 2000; 96(10): 3439 - 3446. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-m. Pasquet, B. S. Gross, M.-P. Gratacap, L. Quek, S. Pasquet, B. Payrastre, G. van Willigen, J. C. Mountford, and S. P. Watson Thrombopoietin potentiates collagen receptor signaling in platelets through a phosphatidylinositol 3-kinase-dependent pathway Blood, June 1, 2000; 95(11): 3429 - 3434. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Trumel, B. Payrastre, M. Plantavid, B. Hechler, C. Viala, P. Presek, E. A. Martinson, J.-P. Cazenave, H. Chap, and C. Gachet A Key Role of Adenosine Diphosphate in the Irreversible Platelet Aggregation Induced by the PAR1-Activating Peptide Through the Late Activation of Phosphoinositide 3-Kinase Blood, December 15, 1999; 94(12): 4156 - 4165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Law, L. Nannizzi-Alaimo, K. Ministri, P. E. Hughes, J. Forsyth, M. Turner, S. J. Shattil, M. H. Ginsberg, V. L.J. Tybulewicz, and D. R. Phillips Genetic and Pharmacological Analyses of Syk Function in alpha IIbbeta 3 Signaling in Platelets Blood, April 15, 1999; 93(8): 2645 - 2652. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Nozu, Y. Tsunoda, A. I. Ibitayo, K. N. Bitar, and C. Owyang Involvement of RhoA and its interaction with protein kinase C and Src in CCK-stimulated pancreatic acini Am J Physiol Gastrointest Liver Physiol, April 1, 1999; 276(4): G915 - G923. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. PETTIT Cytosolic Free Calcium and the Cytoskeleton in the Control of Leukocyte Chemotaxis Physiol Rev, October 1, 1998; 78(4): 949 - 967. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. JANMEY The Cytoskeleton and Cell Signaling: Component Localization and Mechanical Coupling Physiol Rev, July 1, 1998; 78(3): 763 - 781. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Leng, H. Kashiwagi, X.-D. Ren, and S. J. Shattil RhoA and the Function of Platelet Integrin alpha IIbbeta 3 Blood, June 1, 1998; 91(11): 4206 - 4215. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Giuriato, B. Payrastre, A. L. Drayer, M. Plantavid, R. Woscholski, P. Parker, C. Erneux, and H. Chap Tyrosine Phosphorylation and Relocation of SHIP Are Integrin-mediated in Thrombin-stimulated Human Blood Platelets J. Biol. Chem., October 24, 1997; 272(43): 26857 - 26863. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Defacque, E. Bos, B. Garvalov, C. Barret, C. Roy, P. Mangeat, H.-W. Shin, V. Rybin, and G. Griffiths Phosphoinositides Regulate Membrane-dependent Actin Assembly by Latex Bead Phagosomes Mol. Biol. Cell, April 1, 2002; 13(4): 1190 - 1202. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
