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(Received for publication, October 9, 1996, and in revised form, November 22, 1996)
From the Institut für Biologie III der Universität
Freiburg, Expression of the chicken lysozyme locus in
macrophages is regulated by at least six different positive and
negative cis-regulatory elements. Chromatin of the chicken lysozyme
locus is gradually reorganized during macrophage differentiation,
indicating that each cis-regulatory element is activated at a different
developmental stage. Irrespective of their differential developmental
activation, individual cis-regulatory regions are capable of driving
transcription of the lysozyme gene in mature macrophages of transgenic
mice. In order to examine the role of different cis-regulatory regions in lysozyme locus activation, we analyzed the time course of
transcriptional up-regulation of deletion mutants of the lysozyme locus
in a new in vitro differentiation system based on enriched
primary macrophage precursor cells from the bone marrow of transgenic
mice. We show that constructs carrying cis-regulatory elements which
are structurally reorganized early in development are also
transcriptionally active at an early stage. A construct in which the
early enhancer has been deleted shows a delay in transcriptional
activation. The presence or absence of a negative regulatory element
has no influence on the time course of transcriptional activation of
the lysozyme locus.
Volume 272, Number 9,
Issue of February 28, 1997
pp. 5871-5879
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
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