Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jägle, U.
Right arrow Articles by Bonifer, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jägle, U.
Right arrow Articles by Bonifer, C.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Volume 272, Number 9, Issue of February 28, 1997 pp. 5871-5879
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Role of Positive and Negative Cis-regulatory Elements in the Transcriptional Activation of the Lysozyme Locus in Developing Macrophages of Transgenic Mice

(Received for publication, October 9, 1996, and in revised form, November 22, 1996)

Ulrike Jägle , Albrecht M. Müller § , Hubertus Kohler § and Constanze Bonifer

From the Institut für Biologie III der Universität Freiburg, Schänzlestrasse 1, D-79104 Freiburg and § Max Planck Institut für Immunbiologie, Stübeweg 51, 79108 Freiburg, Federal Republic of Germany

Expression of the chicken lysozyme locus in macrophages is regulated by at least six different positive and negative cis-regulatory elements. Chromatin of the chicken lysozyme locus is gradually reorganized during macrophage differentiation, indicating that each cis-regulatory element is activated at a different developmental stage. Irrespective of their differential developmental activation, individual cis-regulatory regions are capable of driving transcription of the lysozyme gene in mature macrophages of transgenic mice. In order to examine the role of different cis-regulatory regions in lysozyme locus activation, we analyzed the time course of transcriptional up-regulation of deletion mutants of the lysozyme locus in a new in vitro differentiation system based on enriched primary macrophage precursor cells from the bone marrow of transgenic mice. We show that constructs carrying cis-regulatory elements which are structurally reorganized early in development are also transcriptionally active at an early stage. A construct in which the early enhancer has been deleted shows a delay in transcriptional activation. The presence or absence of a negative regulatory element has no influence on the time course of transcriptional activation of the lysozyme locus.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
BloodHome page
H. Tagoh, S. Melnik, P. Lefevre, S. Chong, A. D. Riggs, and C. Bonifer
Dynamic reorganization of chromatin structure and selective DNA demethylation prior to stable enhancer complex formation during differentiation of primary hematopoietic cells in vitro
Blood, April 15, 2004; 103(8): 2950 - 2955.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
P. Lefevre, S. Melnik, N. Wilson, A. D. Riggs, and C. Bonifer
Developmentally Regulated Recruitment of Transcription Factors and Chromatin Modification Activities to Chicken Lysozyme cis-Regulatory Elements In Vivo
Mol. Cell. Biol., June 15, 2003; 23(12): 4386 - 4400.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
S. Chong, J. Kontaraki, C. Bonifer, and A. D. Riggs
A Functional Chromatin Domain Does Not Resist X Chromosome Inactivation: Silencing of cLys Correlates with Methylation of a Dual Promoter-Replication Origin
Mol. Cell. Biol., July 1, 2002; 22(13): 4667 - 4676.
[Abstract] [Full Text] [PDF]


Home page
Genes Dev.Home page
H. Tagoh, R. Himes, D. Clarke, P. J.M. Leenen, A. D. Riggs, D. Hume, and C. Bonifer
Transcription factor complex formation and chromatin fine structure alterations at the murine c-fms (CSF-1 receptor) locus during maturation of myeloid precursor cells
Genes & Dev., July 1, 2002; 16(13): 1721 - 1737.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
P. Lefevre, J. Kontaraki, and C. Bonifer
Identification of factors mediating the developmental regulation of the early acting -3.9 kb chicken lysozyme enhancer element
Nucleic Acids Res., November 15, 2001; 29(22): 4551 - 4560.
[Abstract] [Full Text] [PDF]


Home page
Genes Dev.Home page
J. Kontaraki, H.-H. Chen, A. Riggs, and C. Bonifer
Chromatin fine structure profiles for a developmentally regulated gene: reorganization of the lysozyme locus before trans-activator binding and gene expression
Genes & Dev., August 15, 2000; 14(16): 2106 - 2122.
[Abstract] [Full Text]


Home page
J. Immunol.Home page
R. Goethe and L. Phi-van
Posttranscriptional Lipopolysaccharide Regulation of the Lysozyme Gene at Processing of the Primary Transcript in Myelomonocytic HD11 Cells
J. Immunol., May 15, 1998; 160(10): 4970 - 4978.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
C. Bonifer, U. Jagle, and M. C. Huber
The Chicken Lysozyme Locus as a Paradigm for the Complex Developmental Regulation of Eukaryotic Gene Loci
J. Biol. Chem., October 17, 1997; 272(42): 26075 - 26078.
[Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1997 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement