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(Received for publication, September 13, 1996, and in revised form, November 18, 1996)
From the Intracisternal A-particle (IAP) sequences are
endogenous retrovirus-like elements present at 1,000 copies in the
mouse genome. We had previously identified IAP-related transcripts of
unusual size (6 and 10 kilobases (kb)), which are observed exclusively in the liver of the aging mouse. In this report, using cDNA
libraries that we have constructed from the liver mRNAs of an aged
DBA/2 mouse, we have cloned and entirely sequenced the corresponding cDNAs. Both are initiated within the 5
Volume 272, Number 9,
Issue of February 28, 1997
pp. 5995-6003
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
TRANSCRIPTIONAL READ-THROUGH INTO AN OPEN READING FRAME WITH
SIMILARITIES TO THE YEAST CCR4 TRANSCRIPTION FACTOR
,
,
,
and
Unité de Physicochimie et
Pharmacologie des Macromolécules Biologiques,
Unité de Génétique Médicale et
Développement,
long terminal repeat of a
type I
1 IAP sequence, and correspond to a read-through into a unique
flanking cellular sequence containing a 966-nucleotide open reading
frame, located 3 to the IAP sequence. The 6-kb IAP-related transcript
corresponds to a post-transcriptional modification of the 10-kb
mRNA, and is generated by a splicing event with the donor site in
the IAP sequence, and the acceptor site 5 to the open reading frame.
This open reading frame is located on chromosome 3, is evolutionarily
conserved, and discloses significant similarity to the yeast CCR4
transcription factor at the amino acid level. The specific expression
of these age-induced transcripts, which account for more than 50% of
the IAP-related transcripts in the liver of old mice, is therefore
entirely consistent with the induction of a single genomic locus, thus
strengthening the importance of position effects for the expression of
transposable elements. Characterization of this locus should now allow
studies on its chromatin and methylation status, and on the
"molecular factors of senescence" possibly involved in its
induction.
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