JBC Avanti Polar Lipids

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Spady, D. K.
Right arrow Articles by Meidell, R. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Spady, D. K.
Right arrow Articles by Meidell, R. S.

Vol. 273, Issue 1, 126-132, January 2, 1998

Overexpression of Cholesterol 7alpha -Hydroxylase (CYP7A) in Mice Lacking the Low Density Lipoprotein (LDL) Receptor Gene
LDL TRANSPORT AND PLASMA LDL CONCENTRATIONS ARE REDUCED

David K. Spady, Jennifer A. Cuthbert, Maureen N. Willard, and Robert S. Meidell

From the Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75235-8887

This study was undertaken to determine the effect of transient overexpression of hepatic cholesterol 7alpha -hydroxylase on low density lipoprotein (LDL) cholesterol transport in mice lacking LDL receptors (LDL receptor-/-). Primary overexpression of hepatic 7alpha -hydroxylase in LDL receptor-/- mice was accompanied by a dose-dependent decrease in the rate of LDL cholesterol appearance in plasma (whole body LDL cholesterol transport) and a corresponding reduction in circulating LDL cholesterol levels. The increase in hepatic 7alpha -hydroxylase activity necessary to achieve a 50% reduction in plasma LDL cholesterol concentrations was ~10-fold. In comparison, cholestyramine increased hepatic 7alpha -hydroxylase activity ~3-fold and reduced plasma LDL cholesterol concentrations by 17%. This study demonstrates that augmentation of hepatic 7alpha -hydroxylase expression is an effective strategy for lowering plasma LDL concentrations even in animals with a genetic absence of LDL receptors.


Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.



This article has been cited by other articles:


Home page
Exp. Biol. Med.Home page
G. C. Ness and K. R. Gertz
Hepatic HMG-CoA Reductase Expression and Resistance to Dietary Cholesterol
Experimental Biology and Medicine, May 1, 2004; 229(5): 412 - 416.
[Abstract] [Full Text] [PDF]


Home page
Arterioscler. Thromb. Vasc. Bio.Home page
S. Lind, M. Rudling, S. Ericsson, H. Olivecrona, M. Eriksson, B. Borgstrom, G. Eggertsen, L. Berglund, and B. Angelin
Growth Hormone Induces Low-Density Lipoprotein Clearance but not Bile Acid Synthesis in Humans
Arterioscler. Thromb. Vasc. Biol., February 1, 2004; 24(2): 349 - 356.
[Abstract] [Full Text]


Home page
J. Biol. Chem.Home page
E. De Fabiani, N. Mitro, F. Gilardi, D. Caruso, G. Galli, and M. Crestani
Coordinated Control of Cholesterol Catabolism to Bile Acids and of Gluconeogenesis via a Novel Mechanism of Transcription Regulation Linked to the Fasted-to-fed Cycle
J. Biol. Chem., October 3, 2003; 278(40): 39124 - 39132.
[Abstract] [Full Text] [PDF]


Home page
J. Lipid Res.Home page
S. K. Erickson, S. R. Lear, S. Deane, S. Dubrac, S. L. Huling, L. Nguyen, J. S. Bollineni, S. Shefer, H. Hyogo, D. E. Cohen, et al.
Hypercholesterolemia and changes in lipid and bile acid metabolism in male and female cyp7A1-deficient mice
J. Lipid Res., May 1, 2003; 44(5): 1001 - 1009.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Fuchs
Bile Acid Regulation of Hepatic Physiology: III. Regulation of bile acid synthesis: past progress and future challenges
Am J Physiol Gastrointest Liver Physiol, April 1, 2003; 284(4): G551 - G557.
[Abstract] [Full Text] [PDF]


Home page
Endocr. Rev.Home page
J. Y. L. Chiang
Bile Acid Regulation of Gene Expression: Roles of Nuclear Hormone Receptors
Endocr. Rev., August 1, 2002; 23(4): 443 - 463.
[Abstract] [Full Text] [PDF]


Home page
J. Lipid Res.Home page
C. Vasandani, A. I. Kafrouni, A. Caronna, Y. Bashmakov, M. Gotthardt, J. D. Horton, and D. K. Spady
Upregulation of hepatic LDL transport by n-3 fatty acids in LDL receptor knockout mice
J. Lipid Res., May 1, 2002; 43(5): 772 - 784.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
J. Phan, T. Pesaran, R. C. Davis, and K. Reue
The Diet1 Locus Confers Protection against Hypercholesterolemia through Enhanced Bile Acid Metabolism
J. Biol. Chem., January 4, 2002; 277(1): 469 - 477.
[Abstract] [Full Text]


Home page
Am. J. Physiol. Gastrointest. Liver Physiol.Home page
W. M. Pandak, C. Schwarz, P. B. Hylemon, D. Mallonee, K. Valerie, D. M. Heuman, R. A. Fisher, K. Redford, and Z. R. Vlahcevic
Effects of CYP7A1 overexpression on cholesterol and bile acid homeostasis
Am J Physiol Gastrointest Liver Physiol, October 1, 2001; 281(4): G878 - G889.
[Abstract] [Full Text] [PDF]


Home page
FASEB J.Home page
M. RUDLING and B. ANGELIN
Growth hormone reduces plasma cholesterol in LDL receptor-deficient mice
FASEB J, June 1, 2001; 15(8): 1350 - 1356.
[Abstract] [Full Text] [PDF]


Home page
Exp. Biol. Med.Home page
G. C. Ness and C. M. Chambers
Feedback and Hormonal Regulation of Hepatic 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase: The Concept of Cholesterol Buffering Capacity
Experimental Biology and Medicine, May 1, 2000; 224(1): 8 - 19.
[Abstract] [Full Text]


Home page
J. Pharmacol. Exp. Ther.Home page
N. N. Izzat, M. E. Deshazer, and D. S. Loose-Mitchell
New Molecular Targets for Cholesterol-Lowering Therapy
J. Pharmacol. Exp. Ther., May 1, 2000; 293(2): 315 - 320.
[Abstract] [Full Text]


Home page
Physiol. Rev.Home page
G. J. Schroepfer Jr.
Oxysterols: Modulators of Cholesterol Metabolism and Other Processes
Physiol Rev, January 1, 2000; 80(1): 361 - 554.
[Abstract] [Full Text] [PDF]


Home page
J. Lipid Res.Home page
P. Couture, J. D. Otvos, L. A. Cupples, P. W. F. Wilson, E. J. Schaefer, and J. M. Ordovas
Association of the A-204C polymorphism in the cholesterol 7{alpha}-hydroxylase gene with variations in plasma low density lipoprotein cholesterol levels in the Framingham Offspring Study
J. Lipid Res., October 1, 1999; 40(10): 1883 - 1889.
[Abstract] [Full Text]


Home page
J. Biol. Chem.Home page
T. Arai, N. Wang, M. Bezouevski, C. Welch, and A. R. Tall
Decreased Atherosclerosis in Heterozygous Low Density Lipoprotein Receptor-deficient Mice Expressing the Scavenger Receptor BI Transgene
J. Biol. Chem., January 22, 1999; 274(4): 2366 - 2371.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
N. Wang, T. Arai, Y. Ji, F. Rinninger, and A. R. Tall
Liver-specific Overexpression of Scavenger Receptor BI Decreases Levels of Very Low Density Lipoprotein ApoB, Low Density Lipoprotein ApoB, and High Density Lipoprotein in Transgenic Mice
J. Biol. Chem., December 4, 1998; 273(49): 32920 - 32926.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
D. K. Spady, M. N. Willard, and R. S. Meidell
Role of Acyl-Coenzyme A:Cholesterol Acyltransferase-1 in the Control of Hepatic Very Low Density Lipoprotein Secretion and Low Density Lipoprotein Receptor Expression in the Mouse and Hamster
J. Biol. Chem., August 25, 2000; 275(35): 27005 - 27012.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
K. Alam, R. S. Meidell, and D. K. Spady
Effect of Up-regulating Individual Steps in the Reverse Cholesterol Transport Pathway on Reverse Cholesterol Transport in Normolipidemic Mice
J. Biol. Chem., May 4, 2001; 276(19): 15641 - 15649.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
R. A. Memon, A. H. Moser, J. K. Shigenaga, C. Grunfeld, and K. R. Feingold
In Vivo and in Vitro Regulation of Sterol 27-Hydroxylase in the Liver during the Acute Phase Response. POTENTIAL ROLE OF HEPATOCYTE NUCLEAR FACTOR-1
J. Biol. Chem., August 3, 2001; 276(32): 30118 - 30126.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1998 by the American Society for Biochemistry and Molecular Biology.