|
|
|
|||||||
|
|||||||||
Vol. 273, Issue 1, 85-91, January 2, 1998
From the Bacteria able to grow in aqueous:organic
two-phase systems have evolved resistance mechanisms to the toxic
effects of solvents. One such mechanism is the active efflux of
solvents from the cell, preserving the integrity of the cell interior.
Pseudomonas putida S12 is resistant to a wide variety of
normally detrimental solvents due to the action of such an efflux pump.
The genes for this solvent efflux pump were cloned from P. putida S12 and their nucleotide sequence determined. The deduced
amino acid sequences encoded by the three genes involved show a
striking resemblance to proteins known to be involved in
proton-dependent multidrug efflux systems. Transfer of the
genes for the solvent efflux pump to solvent-sensitive P. putida strains results in the acquisition of solvent resistance. This opens up the possibilities of using the solvent efflux system to
construct bacterial strains capable of performing biocatalytic transformations of insoluble substrates in two-phase aqueous:organic medium.
Identification and Molecular Characterization of an Efflux Pump
Involved in Pseudomonas putida S12 Solvent Tolerance
,, and Division of Industrial Microbiology,
Department of Food Science, Wageningen Agricultural University,
Wageningen, The Netherlands and the § Center for
Agricultural Molecular Biology, Cook College, Rutgers University,
New Brunswick, New Jersey 08903-0231
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Fillet, M. Velez, D. Lu, X. Zhang, M.-T. Gallegos, and J. L. Ramos TtgV Represses Two Different Promoters by Recognizing Different Sequences J. Bacteriol., March 15, 2009; 191(6): 1901 - 1909. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. P. Wierckx, H. Ballerstedt, J. A. M. de Bont, J. H. de Winde, H. J. Ruijssenaars, and J. Wery Transcriptome Analysis of a Phenol-Producing Pseudomonas putida S12 Construct: Genetic and Physiological Basis for Improved Production J. Bacteriol., April 15, 2008; 190(8): 2822 - 2830. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Kivistik, M. Putrins, K. Puvi, H. Ilves, M. Kivisaar, and R. Horak The ColRS Two-Component System Regulates Membrane Functions and Protects Pseudomonas putida against Phenol J. Bacteriol., December 1, 2006; 188(23): 8109 - 8117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kieboom and T. Abee Arginine-Dependent Acid Resistance in Salmonella enterica Serovar Typhimurium. J. Bacteriol., August 1, 2006; 188(15): 5650 - 5653. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Hearn, M. R. Gray, and J. M. Foght Mutations in the Central Cavity and Periplasmic Domain Affect Efflux Activity of the Resistance-Nodulation-Division Pump EmhB from Pseudomonas fluorescens cLP6a J. Bacteriol., January 1, 2006; 188(1): 115 - 123. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. J. P. Wierckx, H. Ballerstedt, J. A. M. de Bont, and J. Wery Engineering of Solvent-Tolerant Pseudomonas putida S12 for Bioproduction of Phenol from Glucose Appl. Envir. Microbiol., December 1, 2005; 71(12): 8221 - 8227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Segura, P. Godoy, P. van Dillewijn, A. Hurtado, N. Arroyo, S. Santacruz, and J.-L. Ramos Proteomic Analysis Reveals the Participation of Energy- and Stress-Related Proteins in the Response of Pseudomonas putida DOT-T1E to Toluene J. Bacteriol., September 1, 2005; 187(17): 5937 - 5945. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Ramos, M. Martinez-Bueno, A. J. Molina-Henares, W. Teran, K. Watanabe, X. Zhang, M. T. Gallegos, R. Brennan, and R. Tobes The TetR Family of Transcriptional Repressors Microbiol. Mol. Biol. Rev., June 1, 2005; 69(2): 326 - 356. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Meguro, Y. Kodama, M.-T. Gallegos, and K. Watanabe Molecular Characterization of Resistance-Nodulation-Division Transporters from Solvent- and Drug-Resistant Bacteria in Petroleum-Contaminated Soil Appl. Envir. Microbiol., January 1, 2005; 71(1): 580 - 586. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. He and R. A. Sanford Acetate Threshold Concentrations Suggest Varying Energy Requirements during Anaerobic Respiration by Anaeromyxobacter dehalogenans Appl. Envir. Microbiol., November 1, 2004; 70(11): 6940 - 6943. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Van Hamme, A. Singh, and O. P. Ward Recent Advances in Petroleum Microbiology Microbiol. Mol. Biol. Rev., December 1, 2003; 67(4): 503 - 549. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Hearn, J. J. Dennis, M. R. Gray, and J. M. Foght Identification and Characterization of the emhABC Efflux System for Polycyclic Aromatic Hydrocarbons in Pseudomonas fluorescens cLP6a J. Bacteriol., November 1, 2003; 185(21): 6233 - 6240. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Teran, A. Felipe, A. Segura, A. Rojas, J.-L. Ramos, and M.-T. Gallegos Antibiotic-Dependent Induction of Pseudomonas putida DOT-T1E TtgABC Efflux Pump Is Mediated by the Drug Binding Repressor TtgR Antimicrob. Agents Chemother., October 1, 2003; 47(10): 3067 - 3072. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. W. Yu, J. R. Aires, and H. Nikaido AcrB Multidrug Efflux Pump of Escherichia coli: Composite Substrate-Binding Cavity of Exceptional Flexibility Generates Its Extremely Wide Substrate Specificity J. Bacteriol., October 1, 2003; 185(19): 5657 - 5664. [Full Text] [PDF]
|
|
|
|
|
|
A. Rojas, A. Segura, M. E. Guazzaroni, W. Teran, A. Hurtado, M. T. Gallegos, and J. L. Ramos In Vivo and In Vitro Evidence that TtgV Is the Specific Regulator of the TtgGHI Multidrug and Solvent Efflux Pump of Pseudomonas putida J. Bacteriol., August 15, 2003; 185(16): 4755 - 4763. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Abe, T. Okutsu, H. Nakajima, N. Kakuda, I. Ohtsu, and R. Aono n-Hexane sensitivity of Escherichia coli due to low expression of imp/ostA encoding an 87 kDa minor protein associated with the outer membrane Microbiology, May 1, 2003; 149(5): 1265 - 1273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Grkovic, M. H. Brown, and R. A. Skurray Regulation of Bacterial Drug Export Systems Microbiol. Mol. Biol. Rev., December 1, 2002; 66(4): 671 - 701. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-Z. Li, L. Zhang, and K. Poole SmeC, an Outer Membrane Multidrug Efflux Protein of Stenotrophomonas maltophilia Antimicrob. Agents Chemother., February 1, 2002; 46(2): 333 - 343. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Fontaine, I. Meynial-Salles, L. Girbal, X. Yang, C. Croux, and P. Soucaille Molecular Characterization and Transcriptional Analysis of adhE2, the Gene Encoding the NADH-Dependent Aldehyde/Alcohol Dehydrogenase Responsible for Butanol Production in Alcohologenic Cultures of Clostridium acetobutylicum ATCC 824 J. Bacteriol., February 1, 2002; 184(3): 821 - 830. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Tsuge, Y. Ohata, and M. Shoda Gene yerP, Involved in Surfactin Self-Resistance in Bacillus subtilis Antimicrob. Agents Chemother., December 1, 2001; 45(12): 3566 - 3573. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-I. Ramos-Gonzalez, P. Godoy, M. Alaminos, A. Ben-Bassat, and J.-L. Ramos Physiological Characterization of Pseudomonas putida DOT-T1E Tolerance to p-Hydroxybenzoate Appl. Envir. Microbiol., September 1, 2001; 67(9): 4338 - 4341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Segura, E. Duque, A. Hurtado, and J. L. Ramos Mutations in Genes Involved in the Flagellar Export Apparatus of the Solvent-Tolerant Pseudomonas putida DOT-T1E Strain Impair Motility and Lead to Hypersensitivity to Toluene Shocks J. Bacteriol., July 15, 2001; 183(14): 4127 - 4133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rojas, E. Duque, G. Mosqueda, G. Golden, A. Hurtado, J. L. Ramos, and A. Segura Three Efflux Pumps Are Required To Provide Efficient Tolerance to Toluene in Pseudomonas putida DOT-T1E J. Bacteriol., July 1, 2001; 183(13): 3967 - 3973. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-Z. Li and K. Poole Mutational Analysis of the OprM Outer Membrane Component of the MexA-MexB-OprM Multidrug Efflux System of Pseudomonas aeruginosa J. Bacteriol., January 1, 2001; 183(1): 12 - 27. [Abstract] [Full Text]
|
|
|
|
|
|
J. Kieboom and J. A. M. de Bont Identification and molecular characterization of an efflux system involved in Pseudomonas putida S12 multidrug resistance Microbiology, January 1, 2001; 147(1): 43 - 51. [Abstract] [Full Text]
|
|
|
|
|
|
H. Kobayashi, K. Uematsu, H. Hirayama, and K. Horikoshi Novel Toluene Elimination System in a Toluene-Tolerant Microorganism J. Bacteriol., November 15, 2000; 182(22): 6451 - 6455. [Abstract] [Full Text]
|
|
|
|
|
|
D. R. Kadavy, J. M. Hornby, T. Haverkost, and K. W. Nickerson Natural Antibiotic Resistance of Bacteria Isolated from Larvae of the Oil Fly, Helaeomyia petrolei Appl. Envir. Microbiol., November 1, 2000; 66(11): 4615 - 4619. [Abstract] [Full Text]
|
|
|
|
|
|
N. Tsukagoshi and R. Aono Entry into and Release of Solvents by Escherichia coli in an Organic-Aqueous Two-Liquid-Phase System and Substrate Specificity of the AcrAB-TolC Solvent-Extruding Pump J. Bacteriol., September 1, 2000; 182(17): 4803 - 4810. [Abstract] [Full Text]
|
|
|
|
|
|
K. K. Y. Wong and R. E. W. Hancock Insertion Mutagenesis and Membrane Topology Model of the Pseudomonas aeruginosa Outer Membrane Protein OprM J. Bacteriol., May 1, 2000; 182(9): 2402 - 2410. [Abstract] [Full Text]
|
|
|
|
|
|
G. Mosqueda and J.-L. Ramos A Set of Genes Encoding a Second Toluene Efflux System in Pseudomonas putida DOT-T1E Is Linked to the tod Genes for Toluene Metabolism J. Bacteriol., February 15, 2000; 182(4): 937 - 943. [Abstract] [Full Text]
|
|
|
|
|
|
L. Zhang, X.-Z. Li, and K. Poole Multiple Antibiotic Resistance in Stenotrophomonas maltophilia: Involvement of a Multidrug Efflux System Antimicrob. Agents Chemother., February 1, 2000; 44(2): 287 - 293. [Abstract] [Full Text]
|
|
|
|
|
|
F. Junker and J. L. Ramos Involvement of the cis/trans Isomerase Cti in Solvent Resistance of Pseudomonas putida DOT-T1E J. Bacteriol., September 15, 1999; 181(18): 5693 - 5700. [Abstract] [Full Text]
|
|
|
|
|
|
H. Kobayashi, H. Takami, H. Hirayama, K. Kobata, R. Usami, and K. Horikoshi Outer Membrane Changes in a Toluene-Sensitive Mutant of Toluene-Tolerant Pseudomonas putida IH-2000 J. Bacteriol., August 1, 1999; 181(15): 4493 - 4498. [Abstract] [Full Text]
|
|
|
|
|
|
S. Isken, A. Derks, P. F. G. Wolffs, and J. A. M. de Bont Effect of Organic Solvents on the Yield of Solvent-Tolerant Pseudomonas putida S12 Appl. Envir. Microbiol., June 1, 1999; 65(6): 2631 - 2635. [Abstract] [Full Text]
|
|
|
|
|
|
H.-K. Chang and G. J. Zylstra Role of Quinolinate Phosphoribosyl Transferase in Degradation of Phthalate by Burkholderia cepacia DBO1 J. Bacteriol., May 15, 1999; 181(10): 3069 - 3075. [Abstract] [Full Text]
|
|
|
|
|
|
V. Pedrotta and B. Witholt Isolation and Characterization of the cis-trans-Unsaturated Fatty Acid Isomerase of Pseudomonas oleovorans GPo12 J. Bacteriol., May 15, 1999; 181(10): 3256 - 3261. [Abstract] [Full Text]
|
|
|
|
|
|
M. F. Romine, L. C. Stillwell, K.-K. Wong, S. J. Thurston, E. C. Sisk, C. Sensen, T. Gaasterland, J. K. Fredrickson, and J. D. Saffer Complete Sequence of a 184-Kilobase Catabolic Plasmid from Sphingomonas aromaticivorans F199 J. Bacteriol., March 1, 1999; 181(5): 1585 - 1602. [Abstract] [Full Text]
|
|
|
|
|
|
J. P. Pearson, C. Van Delden, and B. H. Iglewski Active Efflux and Diffusion Are Involved in Transport of Pseudomonas aeruginosa Cell-to-Cell Signals J. Bacteriol., February 15, 1999; 181(4): 1203 - 1210. [Abstract] [Full Text]
|
|
|
|
|
|
N. Masuda, N. Gotoh, C. Ishii, E. Sakagawa, S. Ohya, and T. Nishino Interplay between Chromosomal beta -Lactamase and the MexAB-OprM Efflux System in Intrinsic Resistance to beta -Lactams in Pseudomonas aeruginosa Antimicrob. Agents Chemother., February 1, 1999; 43(2): 400 - 402. [Abstract] [Full Text]
|
|
|
|
|
|
H. Asako, K. Kobayashi, and R. Aono Organic Solvent Tolerance of Escherichia coli Is Independent of OmpF Levels in the Membrane Appl. Envir. Microbiol., January 1, 1999; 65(1): 294 - 296. [Abstract] [Full Text]
|
|
|
|
|
|
J. Kieboom, J. J. Dennis, G. J. Zylstra, and J. A. M. de Bont Active Efflux of Organic Solvents by Pseudomonas putida S12 Is Induced by Solvents J. Bacteriol., December 15, 1998; 180(24): 6769 - 6772. [Abstract] [Full Text]
|
|
|
|
|
|
K. Kim, S. Lee, K. Lee, and D. Lim Isolation and Characterization of Toluene-Sensitive Mutants from the Toluene-Resistant Bacterium Pseudomonas putida GM73 J. Bacteriol., July 15, 1998; 180(14): 3692 - 3696. [Abstract] [Full Text]
|
|
|
|
|
|
J. J. Dennis and G. J. Zylstra Plasposons: Modular Self-Cloning Minitransposon Derivatives for Rapid Genetic Analysis of Gram-Negative Bacterial Genomes Appl. Envir. Microbiol., July 1, 1998; 64(7): 2710 - 2715. [Abstract] [Full Text]
|
|
|
|
|
|
J. L. Ramos, E. Duque, P. Godoy, and A. Segura Efflux Pumps Involved in Toluene Tolerance in Pseudomonas putida DOT-T1E J. Bacteriol., July 1, 1998; 180(13): 3323 - 3329. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Wery, B. Hidayat, J. Kieboom, and J. A. M. de Bont An Insertion Sequence Prepares Pseudomonas putida S12 for Severe Solvent Stress J. Biol. Chem., February 16, 2001; 276(8): 5700 - 5706. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |