HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Han, Z. Articles by Patil, R. V. Search for Related Content PubMed PubMed Citation Articles by Han, Z. Articles by Patil, R. V. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 11, 6001-6004, March 13, 1998

COMMUNICATION
Regulation of Aquaporin-4 Water Channels by Phorbol Ester-dependent Protein Phosphorylation

From the Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St. Louis, Missouri 63110

The molecular mechanisms for regulating water balance in many tissues are unknown. Like the kidney, the eye contains multiple water channel proteins (aquaporins) that transport water through membranes, including two (AQP1 and AQP4) in the ciliary body, the site of aqueous humor production. However, because humans with defective AQP1 are phenotypically normal and because the ocular application of phorbol esters reduce intraocular pressure, we postulated that the water channel activity of AQP4 may be regulated by these agents. We now report that protein kinase C activators, phorbol 12,13-dibutyrate, and phorbol 12-myristate 13-acetate strongly stimulate the phosphorylation of AQP4 and inhibit its activity in a dose-dependent manner. Phorbol 12,13-dibutyrate (10 µM) and phorbol 12-myristate 13-acetate (10 nM) reduced the rate of AQP4-expressing oocyte swelling by 87 and 92%, respectively. Further, phorbol 12,13-dibutyrate significantly increased the amount of phosphorylated AQP4. These results demonstrate that protein kinase C can regulate the activity of AQP4 through a mechanism involving protein phosphorylation. Moreover, they suggest important potential roles for AQP4 in several clinical disorders involving rapid water transport such as glaucoma, brain edema, and swelling of premature infant lungs.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				J. D. Ho, R. Yeh, A. Sandstrom, I. Chorny, W. E. C. Harries, R. A. Robbins, L. J. W. Miercke, and R. M. Stroud Crystal structure of human aquaporin 4 at 1.8 A and its mechanism of conductance PNAS, May 5, 2009; 106(18): 7437 - 7442. [Abstract] [Full Text] [PDF]

				W. Zhang, E. Zitron, M. Homme, L. Kihm, C. Morath, D. Scherer, S. Hegge, D. Thomas, C. P. Schmitt, M. Zeier, et al. Aquaporin-1 Channel Function Is Positively Regulated by Protein Kinase C J. Biol. Chem., July 20, 2007; 282(29): 20933 - 20940. [Abstract] [Full Text] [PDF]

				U. Laforenza, E. Cova, G. Gastaldi, S. Tritto, M. Grazioli, N. F. LaRusso, P. L. Splinter, P. D'Adamo, M. Tosco, and U. Ventura Aquaporin-8 Is Involved in Water Transport in Isolated Superficial Colonocytes from Rat Proximal Colon J. Nutr., October 1, 2005; 135(10): 2329 - 2336. [Abstract] [Full Text] [PDF]

				S. A. Grossman, J. B. Alavi, J. G. Supko, K. A. Carson, R. Priet, F. A. Dorr, J. S. Grundy, and J. T. Holmlund Efficacy and toxicity of the antisense oligonucleotide aprinocarsen directed against protein kinase C-{alpha} delivered as a 21-day continuous intravenous infusion in patients with recurrent high-grade astrocytomas Neuro-oncol, January 1, 2005; 7(1): 32 - 40. [Abstract] [PDF]

				M. C. Kenney, S. R. Atilano, N. Zorapapel, B. Holguin, R. N. Gaster, and A. V. Ljubimov Altered Expression of Aquaporins in Bullous Keratopathy and Fuchs' Dystrophy Corneas J. Histochem. Cytochem., October 1, 2004; 52(10): 1341 - 1350. [Abstract] [Full Text] [PDF]

				C. Silberstein, R. Bouley, Y. Huang, P. Fang, N. Pastor-Soler, D. Brown, and A. N. Van Hoek Membrane organization and function of M1 and M23 isoforms of aquaporin-4 in epithelial cells Am J Physiol Renal Physiol, September 1, 2004; 287(3): F501 - F511. [Abstract] [Full Text] [PDF]

				N. C. Connors, M. E. Adams, S. C. Froehner, and P. Kofuji The Potassium Channel Kir4.1 Associates with the Dystrophin-Glycoprotein Complex via {alpha}-Syntrophin in Glia J. Biol. Chem., July 2, 2004; 279(27): 28387 - 28392. [Abstract] [Full Text] [PDF]

				J. F. Guenther, N. Chanmanivone, M. P. Galetovic, I. S. Wallace, J. A. Cobb, and D. M. Roberts Phosphorylation of Soybean Nodulin 26 on Serine 262 Enhances Water Permeability and Is Regulated Developmentally and by Osmotic Signals PLANT CELL, April 1, 2003; 15(4): 981 - 991. [Abstract] [Full Text] [PDF]

				T. Borras, T. V. Morozova, S. L. Heinsohn, R. F. Lyman, T. F. C. Mackay, and R. R. H. Anholt Transcription Profiling in Drosophila Eyes That Overexpress the Human Glaucoma-Associated Trabecular Meshwork-Inducible Glucocorticoid Response Protein/Myocilin (TIGR/MYOC) Genetics, February 1, 2003; 163(2): 637 - 645. [Abstract] [Full Text] [PDF]

				M. Zelenina, S. Zelenin, A. A. Bondar, H. Brismar, and A. Aperia Water permeability of aquaporin-4 is decreased by protein kinase C and dopamine Am J Physiol Renal Physiol, August 1, 2002; 283(2): F309 - F318. [Abstract] [Full Text] [PDF]

				P. Agre, L. S King, M. Yasui, W. B Guggino, O. P. Ottersen, Y. Fujiyoshi, A. Engel, and S. Nielsen Aquaporin water channels - from atomic structure to clinical medicine J. Physiol., July 1, 2002; 542(1): 3 - 16. [Abstract] [Full Text] [PDF]

				G. M. Yanochko and A. J. Yool Regulated Cationic Channel Function in Xenopus Oocytes Expressing Drosophila Big Brain J. Neurosci., April 1, 2002; 22(7): 2530 - 2540. [Abstract] [Full Text] [PDF]

				I. Jo, D. T. Ward, M. A. Baum, J. D. Scott, V. M. Coghlan, T. G. Hammond, and H. W. Harris AQP2 is a substrate for endogenous PP2B activity within an inner medullary AKAP-signaling complex Am J Physiol Renal Physiol, November 1, 2001; 281(5): F958 - F965. [Abstract] [Full Text] [PDF]

				Y. Huang, R. Tracy, G. E. Walsberg, A. Makkinje, P. Fang, D. Brown, and A. N. Van Hoek Absence of aquaporin-4 water channels from kidneys of the desert rodent Dipodomys merriami merriami Am J Physiol Renal Physiol, May 1, 2001; 280(5): F794 - F802. [Abstract] [Full Text] [PDF]

				P. AGRE Aquaporin Water Channels in Kidney J. Am. Soc. Nephrol., April 1, 2000; 11(4): 764 - 777. [Full Text] [PDF]

				A. S. Verkman and A. K. Mitra Structure and function of aquaporin water channels Am J Physiol Renal Physiol, January 1, 2000; 278(1): F13 - F28. [Abstract] [Full Text] [PDF]

				P. Agre, M. Bonhivers, and M. J. Borgnia The Aquaporins, Blueprints for Cellular Plumbing Systems J. Biol. Chem., June 12, 1998; 273(24): 14659 - 14662. [Full Text] [PDF]