| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 12, 6603-6606, March 20, 1998
From the Blood Center of Southeastern Wisconsin,
Milwaukee, Wisconsin 53201
We recently identified a 35-kDa
erythrocyte membrane protein, phospholipid scramblase, that promotes
Ca2+-dependent transbilayer movement of
phosphatidylserine (PS) and other phospholipids (PL) in reconstituted
proteoliposomes (Zhou, Q., Zhao, J., Stout, J. G., Luhm, R. A., Wiedmer, T., and Sims, P. J. (1997) J. Biol. Chem.
272, 18240-18244). To determine whether this same protein is
responsible for the rapid movement of PS from inner-to-outer plasma
membrane leaflets in other cells exposed to elevated cytosolic calcium
concentration ([Ca2+]c), we analyzed how induced
movement of PS to the cell surface related to expression of PL
scramblase. Exposure to Ca2+ ionophore A23187 resulted in
rapid PS exposure in those cell lines constitutively high in PL
scramblase (HEL, Epstein-Barr virus-transformed B-lymphocytes, and
Jurkat), whereas this response was markedly attenuated in cells
expressing low amounts of this protein (Raji, HL60, and Dami). To
confirm this apparent correlation between PL scramblase expression and
PS egress at elevated [Ca2+]c, Raji cells were
transfected with PL scramblase cDNA in pEGFP-C2, and stable
transformants expressing various amounts of GFP-PL scramblase fusion
protein were obtained. Clones expressing GFP-PL scramblase showed
distinctly plasma membrane-localized fluorescence. When compared either
with untransfected Raji cells or with transformants expressing GFP
alone, clones expressing GFP-PL scramblase fusion protein showed
increased exposure of PS at the cell surface in response to elevated
[Ca2+]c, accompanied by increased expression of
membrane catalytic function for the prothrombinase enzyme complex.
These data indicate that transfection with PL scramblase cDNA
promotes movement of PS to cell surfaces and suggest that this protein
normally mediates redistribution of plasma membrane phospholipids in
activated, injured, or apoptotic cells.
This article has been cited by other articles:
|
|
 |
|
  M. Jennewein, M. A. Lewis, D. Zhao, E. Tsyganov, N. Slavine, J. He, L. Watkins, V. D. Kodibagkar, S. O'Kelly, P. Kulkarni, et al. Vascular Imaging of Solid Tumors in Rats with a Radioactive Arsenic-Labeled Antibody that Binds Exposed Phosphatidylserine Clin. Cancer Res., March 1, 2008; 14(5): 1377 - 1385. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Claro, C. A. Kanashiro, M. E. M. Oshiro, A. T. Ferreira, and R. A. Khalil {alpha}- and {epsilon}-Protein Kinase C Activity during Smooth Muscle Cell Apoptosis in Response to {gamma}-Radiation J. Pharmacol. Exp. Ther., September 1, 2007; 322(3): 964 - 972. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. He, T. A. Luster, and P. E. Thorpe Radiation-Enhanced Vascular Targeting of Human Lung Cancers in Mice with a Monoclonal Antibody That Binds Anionic Phospholipids Clin. Cancer Res., September 1, 2007; 13(17): 5211 - 5218. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-M. Chung, O.-N. Bae, K.-M. Lim, J.-Y. Noh, M.-Y. Lee, Y.-S. Jung, and J.-H. Chung Lysophosphatidic Acid Induces Thrombogenic Activity Through Phosphatidylserine Exposure and Procoagulant Microvesicle Generation in Human Erythrocytes Arterioscler. Thromb. Vasc. Biol., February 1, 2007; 27(2): 414 - 421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Phillippe, D. F. Bradley, H. Ji, K. H. Oppenheimer, and E. K. Chien Phospholipid Scramblase Isoform Expression in Pregnant Rat Uterus Reproductive Sciences, October 1, 2006; 13(7): 497 - 501. [Abstract] [PDF]
|
|
|
|
 |
|
 Q. Zhou, I. Ben-Efraim, J.-L. Bigcas, D. Junqueira, T. Wiedmer, and P. J. Sims Phospholipid Scramblase 1 Binds to the Promoter Region of the Inositol 1,4,5-Triphosphate Receptor Type 1 Gene to Enhance Its Expression J. Biol. Chem., October 14, 2005; 280(41): 35062 - 35068. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ran, J. He, X. Huang, M. Soares, D. Scothorn, and P. E. Thorpe Antitumor Effects of a Monoclonal Antibody that Binds Anionic Phospholipids on the Surface of Tumor Blood Vessels in Mice Clin. Cancer Res., February 15, 2005; 11(4): 1551 - 1562. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kwong, R. A. Vaishnav, Y. Liu, N. Subhedar, A. J. Stromberg, M. L. Getchell, and T. V. Getchell Target ablation-induced regulation of macrophage recruitment into the olfactory epithelium of Mip-1{alpha}-/- mice and restoration of function by exogenous MIP-1{alpha} Physiol Genomics, December 15, 2004; 20(1): 73 - 86. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.-W. Zhao, X. Li, Q. Zhao, Y. Huang, D. Li, Z.-G. Peng, W.-Z. Shen, J. Zhao, Q. Zhou, Z. Chen, et al. Protein kinase C{delta} mediates retinoic acid and phorbol myristate acetate-induced phospholipid scramblase 1 gene expression: its role in leukemic cell differentiation Blood, December 1, 2004; 104(12): 3731 - 3738. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Wiedmer, J. Zhao, L. Li, Q. Zhou, A. Hevener, J. M. Olefsky, L. K. Curtiss, and P. J. Sims Adiposity, dyslipidemia, and insulin resistance in mice with targeted deletion of phospholipid scramblase 3 (PLSCR3) PNAS, September 7, 2004; 101(36): 13296 - 13301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-P. Courageot, S. Lepine, M. Hours, F. Giraud, and J.-C. Sulpice Involvement of Sodium in Early Phosphatidylserine Exposure and Phospholipid Scrambling Induced by P2X7 Purinoceptor Activation in Thymocytes J. Biol. Chem., May 21, 2004; 279(21): 21815 - 21823. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Frasch, P. M. Henson, K. Nagaosa, M. B. Fessler, N. Borregaard, and D. L. Bratton Phospholipid Flip-Flop and Phospholipid Scramblase 1 (PLSCR1) Co-localize to Uropod Rafts in Formylated Met-Leu-Phe-stimulated Neutrophils J. Biol. Chem., April 23, 2004; 279(17): 17625 - 17633. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Durant, M. Pederzoli, Y. Lepelletier, S. Canteloup, P. Nusbaum, P. Lesavre, and V. Witko-Sarsat Apoptosis-induced proteinase 3 membrane expression is independent from degranulation J. Leukoc. Biol., January 1, 2004; 75(1): 87 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Dias-Baruffi, H. Zhu, M. Cho, S. Karmakar, R. P. McEver, and R. D. Cummings Dimeric Galectin-1 Induces Surface Exposure of Phosphatidylserine and Phagocytic Recognition of Leukocytes without Inducing Apoptosis J. Biol. Chem., October 17, 2003; 278(42): 41282 - 41293. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Liu, Q. Dai, J. Chen, D. Durrant, A. Freeman, T. Liu, D. Grossman, and R. M. Lee Phospholipid Scramblase 3 Controls Mitochondrial Structure, Function, and Apoptotic Response Mol. Cancer Res., October 1, 2003; 1(12): 892 - 902. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Gargalovic and L. Dory Cellular apoptosis is associated with increased caveolin-1 expression in macrophages J. Lipid Res., September 1, 2003; 44(9): 1622 - 1632. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. E. Kagan, G. G. Borisenko, B. F. Serinkan, Y. Y. Tyurina, V. A. Tyurin, J. Jiang, S. X. Liu, A. A. Shvedova, J. P. Fabisiak, W. Uthaisang, et al. Appetizing rancidity of apoptotic cells for macrophages: oxidation, externalization, and recognition of phosphatidylserine Am J Physiol Lung Cell Mol Physiol, July 1, 2003; 285(1): L1 - L17. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kametaka, M. Shibata, K. Moroe, S. Kanamori, Y. Ohsawa, S. Waguri, P. J. Sims, K. Emoto, M. Umeda, and Y. Uchiyama Identification of Phospholipid Scramblase 1 as a Novel Interacting Molecule with beta -Secretase (beta -Site Amyloid Precursor Protein (APP) Cleaving Enzyme (BACE)) J. Biol. Chem., April 18, 2003; 278(17): 15239 - 15245. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ran, A. Downes, and P. E. Thorpe Increased Exposure of Anionic Phospholipids on the Surface of Tumor Blood Vessels Cancer Res., November 1, 2002; 62(21): 6132 - 6140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Andrews, L. Yang, and P. S. Low Phorbol ester stimulates a protein kinase C-mediated agatoxin-TK-sensitive calcium permeability pathway in human red blood cells Blood, October 16, 2002; 100(9): 3392 - 3399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Zhang, R. C. M. Simmen, F. J. Michel, G. Zhao, D. Vale-Cruz, and F. A. Simmen Secretory Leukocyte Protease Inhibitor Mediates Proliferation of Human Endometrial Epithelial Cells by Positive and Negative Regulation of Growth-associated Genes J. Biol. Chem., August 9, 2002; 277(33): 29999 - 30009. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Zhou, J. Zhao, T. Wiedmer, and P. J. Sims Normal hemostasis but defective hematopoietic response to growth factors in mice deficient in phospholipid scramblase 1 Blood, May 13, 2002; 99(11): 4030 - 4038. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. H. Silverman, A. Halloum, A. Zhou, B. Dong, F. Al-Zoghaibi, D. Kushner, Q. Zhou, J. Zhao, T. Wiedmer, and P. J. Sims Suppression of Ovarian Carcinoma Cell Growth in Vivo by the Interferon-inducible Plasma Membrane Protein, Phospholipid Scramblase 1 Cancer Res., January 1, 2002; 62(2): 397 - 402. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. D. Tepper, P. Ruurs, T. Wiedmer, P. J. Sims, J. Borst, and W. J. van Blitterswijk Sphingomyelin Hydrolysis to Ceramide during the Execution Phase of Apoptosis Results from Phospholipid Scrambling and Alters Cell-surface Morphology J. Cell Biol., July 11, 2000; 150(1): 155 - 164. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. H.-F. Chang, N. M. Barbaro, and R. O. Pieper Phosphatidylserine-dependent phagocytosis of apoptotic glioma cells by normal human microglia, astrocytes, and glioma cells Neuro-oncol, July 1, 2000; 2(3): 174 - 183. [Abstract] [PDF]
|
|
|
|
 |
|
 C. E. Reme, C. Grimm, F. Hafezi, A. Wenzel, and T. P. Williams Apoptosis in the Retina: The Silent Death of Vision Physiology, June 1, 2000; 15(3): 120 - 124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Zhou, J. Zhao, F. Al-Zoghaibi, A. Zhou, T. Wiedmer, R. H. Silverman, and P. J. Sims Transcriptional control of the human plasma membrane phospholipid scramblase 1 gene is mediated by interferon-alpha Blood, April 15, 2000; 95(8): 2593 - 2599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Murakami, T. Kambe, S. Shimbara, K.-i. Higashino, K. Hanasaki, H. Arita, M. Horiguchi, M. Arita, H. Arai, K. Inoue, et al. Different Functional Aspects of the Group II Subfamily (Types IIA and V) and Type X Secretory Phospholipase A2s in Regulating Arachidonic Acid Release and Prostaglandin Generation. IMPLICATIONS OF CYCLOOXYGENASE-2 INDUCTION AND PHOSPHOLIPID SCRAMBLASE-MEDIATED CELLULAR MEMBRANE PERTURBATION J. Biol. Chem., October 29, 1999; 274(44): 31435 - 31444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Bratton, V. A. Fadok, D. A. Richter, J. M. Kailey, S. C. Frasch, T. Nakamura, and P. M. Henson Polyamine Regulation of Plasma Membrane Phospholipid Flip-Flop during Apoptosis J. Biol. Chem., October 1, 1999; 274(40): 28113 - 28120. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. E. Pace, C. Lee, P. L. Stewart, and L. G. Baum Restricted Receptor Segregation into Membrane Microdomains Occurs on Human T Cells During Apoptosis Induced by Galectin-1 J. Immunol., October 1, 1999; 163(7): 3801 - 3811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Shcherbina and E. Remold-O'Donnell Role of Caspase in a Subset of Human Platelet Activation Responses Blood, June 15, 1999; 93(12): 4222 - 4231. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K Boesze-Battaglia and R. Schimmel Collagen-stimulated unidirectional translocation of cholesterol in human platelet membranes J. Exp. Biol., January 2, 1999; 202(4): 453 - 460. [Abstract] [PDF]
|
|
|
|
|
|
S. D. Der, A. Zhou, B. R. G. Williams, and R. H. Silverman Identification of genes differentially regulated by interferon alpha , beta , or gamma using oligonucleotide arrays PNAS, December 22, 1998; 95(26): 15623 - 15628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Zhou, P. J. Sims, and T. Wiedmer Expression of Proteins Controlling Transbilayer Movement of Plasma Membrane Phospholipids in the B Lymphocytes From a Patient With Scott Syndrome Blood, September 1, 1998; 92(5): 1707 - 1712. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. K. Hanson and J. W. Nichols Energy-dependent Flip of Fluorescence-labeled Phospholipids Is Regulated by Nutrient Starvation and Transcription Factors, PDR1 and PDR3 J. Biol. Chem., March 23, 2001; 276(13): 9861 - 9867. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Pastorelli, J. Veiga, N. Charles, E. Voignier, H. Moussu, R. C. Monteiro, and M. Benhamou IgE Receptor Type I-dependent Tyrosine Phosphorylation of Phospholipid Scramblase J. Biol. Chem., June 1, 2001; 276(23): 20407 - 20412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Sun, J. Zhao, M. A. Schwartz, J. Y. J. Wang, T. Wiedmer, and P. J. Sims c-Abl Tyrosine Kinase Binds and Phosphorylates Phospholipid Scramblase 1 J. Biol. Chem., July 27, 2001; 276(31): 28984 - 28990. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
