JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sommer, A.
Right arrow Articles by Lüscher, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sommer, A.
Right arrow Articles by Lüscher, B.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 273, Issue 12, 6632-6642, March 20, 1998

Identification and Characterization of Specific DNA-binding Complexes Containing Members of the Myc/Max/Mad Network of Transcriptional Regulators

Anette Sommer, Kristine Bousset, Elisabeth Kremmer§, Matthias Austen, and Bernhard Lüscher

From the Institut für Molekularbiologie, Medizinische Hochschule Hannover, 30623 Hannover, Germany and § GSF-National Research Center for Environment and Health, Institute of Immunology, 81377 München, Germany

In the past, eukaryotic cell-derived complexes of the Myc/Max/Mad network of transcriptional regulators have largely been refractory to DNA binding studies. We have developed electrophoretic mobility shift assay conditions to measure specific DNA binding of Myc/Max/Mad network complexes using a COS7 cell-based overexpression system. With the established protocol, we have measured on- and off-rates of c-Myc/Max, Max/Max, and Mad1/Max complexes and determined relative affinities. All three complexes appeared to bind with comparable affinity to a Myc E-box sequence. Furthermore, our data derived from competition experiments suggested that the Mad3/Max and Mad4/Max complexes also possess comparable DNA binding affinities. The conditions established for COS7 cell-overexpressed proteins were then used to identify c-Myc/Max, Max/Max, and Mnt/Max complexes in HL-60 cells. However, no Mad1/Max could be detected, despite the induction of Mad1 expression during differentiation. Whereas the DNA binding activity of c-Myc/Max complexes was down-regulated, Max/Max binding increased, and Mnt/Max binding remained unchanged. In addition, we have also tested for upstream stimulatory factor (USF) binding and observed that, in agreement with published data, USF comprises a major Myc E-box-binding factor that is more abundant than any of the Myc/Max/Mad network complexes. Similar to the Mnt/Max complex, the binding activity of USF remained constant during HL-60 differentiation. Our findings establish conditions for the analysis of DNA binding of Myc/Max/Mad complexes and indicate posttranslational regulation of the Max/Max complex.

Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 FASEB J.Home page
S. Rottmann, S. Speckgens, J. Luscher-Firzlaff, and B. Luscher
Inhibition of apoptosis by MAD1 is mediated by repression of the PTEN tumor suppressor gene
FASEB J, April 1, 2008; 22(4): 1124 - 1134.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. Jiang, N. Hein, K. Eckert, J. Luscher-Firzlaff, and B. Luscher
Regulation of the MAD1 promoter by G-CSF
Nucleic Acids Res., March 1, 2008; 36(5): 1517 - 1531.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. Luscher-Firzlaff, I. Gawlista, J. Vervoorts, K. Kapelle, T. Braunschweig, G. Walsemann, C. Rodgarkia-Schamberger, H. Schuchlautz, S. Dreschers, E. Kremmer, et al.
The Human Trithorax Protein hASH2 Functions as an Oncoprotein
Cancer Res., February 1, 2008; 68(3): 749 - 758.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
V. H. Cowling and M. D. Cole
The Myc Transactivation Domain Promotes Global Phosphorylation of the RNA Polymerase II Carboxy-Terminal Domain Independently of Direct DNA Binding
Mol. Cell. Biol., March 15, 2007; 27(6): 2059 - 2073.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
V. H. Cowling, S. Chandriani, M. L. Whitfield, and M. D. Cole
A Conserved Myc Protein Domain, MBIV, Regulates DNA Binding, Apoptosis, Transformation, and G2 Arrest.
Mol. Cell. Biol., June 1, 2006; 26(11): 4226 - 4239.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Mo and M. Henriksson
Identification of small molecules that induce apoptosis in a Myc-dependent manner and inhibit Myc-driven transformation
PNAS, April 18, 2006; 103(16): 6344 - 6349.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. W. Hooker and P. J. Hurlin
Of Myc and Mnt
J. Cell Sci., January 15, 2006; 119(2): 208 - 216.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Krippner-Heidenreich, G. Walsemann, M. J. Beyrouthy, S. Speckgens, R. Kraft, H. Thole, R. V. Talanian, M. M. Hurt, and B. Luscher
Caspase-Dependent Regulation and Subcellular Redistribution of the Transcriptional Modulator YY1 during Apoptosis
Mol. Cell. Biol., May 1, 2005; 25(9): 3704 - 3714.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. W. Park, M. D. M. Huq, H. H. Loh, and L.-N. Wei
Retinoic Acid-Induced Chromatin Remodeling of Mouse {kappa} Opioid Receptor Gene
J. Neurosci., March 30, 2005; 25(13): 3350 - 3357.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Volkening and I. Hoffmann
Involvement of Human MCM8 in Prereplication Complex Assembly by Recruiting hcdc6 to Chromatin
Mol. Cell. Biol., February 15, 2005; 25(4): 1560 - 1568.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. D. Cannon, M. Cherian-Shaw, and C. L. Chaffin
Proliferation of Rat Granulosa Cells during the Periovulatory Interval
Endocrinology, January 1, 2005; 146(1): 414 - 422.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. Liu, L. Li, J. N. Rao, T. Zou, H. M. Zhang, D. Boneva, M. S. Bernard, and J.-Y. Wang
Polyamine-modulated expression of c-myc plays a critical role in stimulation of normal intestinal epithelial cell proliferation
Am J Physiol Cell Physiol, January 1, 2005; 288(1): C89 - C99.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
A. Hultquist, C. Cetinkaya, S. Wu, A. Castell, A. Erlandsson, and L.-G. Larsson
Mad 1 Inhibits Cell Growth and Proliferation but Does Not Promote Differentiation or Overall Survival in Human U-937 Monoblasts
Mol. Cancer Res., August 1, 2004; 2(8): 464 - 476.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
K. T. Smith, B. Coffee, and D. Reines
Occupancy and synergistic activation of the FMR1 promoter by Nrf-1 and Sp1 in vivo
Hum. Mol. Genet., August 1, 2004; 13(15): 1611 - 1621.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. V. Grinberg, C.-D. Hu, and T. K. Kerppola
Visualization of Myc/Max/Mad Family Dimers and the Competition for Dimerization in Living Cells
Mol. Cell. Biol., May 15, 2004; 24(10): 4294 - 4308.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
R. B. Hough and J. Piatigorsky
Preferential Transcription of Rabbit Aldh1a1 in the Cornea: Implication of Hypoxia-Related Pathways
Mol. Cell. Biol., February 1, 2004; 24(3): 1324 - 1340.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
C. L. Chaffin, R. S. Brogan, R. L. Stouffer, and C. A. VandeVoort
Dynamics of Myc/Max/Mad Expression during Luteinization of Primate Granulosa Cells in Vitro: Association with Periovulatory Proliferation
Endocrinology, April 1, 2003; 144(4): 1249 - 1256.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
E Al-azzeh, O Dittrich, J Vervoorts, N Blin, P Gott, and B Luscher
Gastroprotective peptide trefoil factor family 2 gene is activated by upstream stimulating factor but not by c-Myc in gastrointestinal cancer cells
Gut, November 1, 2002; 51(5): 685 - 690.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. W. Park, J. Li, H. H. Loh, and L.-N. Wei
A Novel Signaling Pathway of Nitric Oxide on Transcriptional Regulation of Mouse kappa Opioid Receptor Gene
J. Neurosci., September 15, 2002; 22(18): 7941 - 7947.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. G. Harris, E. White, E. S. Phillips, and K. A. Lillycrop
The Expression of the Developmentally Regulated Proto-oncogene Pax-3 Is Modulated by N-Myc
J. Biol. Chem., September 13, 2002; 277(38): 34815 - 34825.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Uramoto, H. Izumi, T. Ise, M. Tada, T. Uchiumi, M. Kuwano, K. Yasumoto, K. Funa, and K. Kohno
p73 Interacts with c-Myc to Regulate Y-box-binding Protein-1 Expression
J. Biol. Chem., August 23, 2002; 277(35): 31694 - 31702.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. A. Nikiforov, S. Chandriani, B. O'Connell, O. Petrenko, I. Kotenko, A. Beavis, J. M. Sedivy, and M. D. Cole
A Functional Screen for Myc-Responsive Genes Reveals Serine Hydroxymethyltransferase, a Major Source of the One-Carbon Unit for Cell Metabolism
Mol. Cell. Biol., August 15, 2002; 22(16): 5793 - 5800.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. M. Hempen, K. M. Phillips, P. S. Conway, K. H. Sandoval, T. A. Schneeman, H.-J. Wu, and C. S. Kaetzel
Transcriptional Regulation of the Human Polymeric Ig Receptor Gene: Analysis of Basal Promoter Elements
J. Immunol., August 15, 2002; 169(4): 1912 - 1921.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
I. Horikawa, P. L. Cable, S. J. Mazur, E. Appella, C. A. Afshari, and J. C. Barrett
Downstream E-Box-mediated Regulation of the Human Telomerase Reverse Transcriptase (hTERT) Gene Transcription: Evidence for an Endogenous Mechanism of Transcriptional Repression
Mol. Biol. Cell, August 1, 2002; 13(8): 2585 - 2597.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. A. Nikiforov, S. Chandriani, J. Park, I. Kotenko, D. Matheos, A. Johnsson, S. B. McMahon, and M. D. Cole
TRRAP-Dependent and TRRAP-Independent Transcriptional Activation by Myc Family Oncoproteins
Mol. Cell. Biol., July 15, 2002; 22(14): 5054 - 5063.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
L. Soucek, R. Jucker, L. Panacchia, R. Ricordy, F. Tato, and S. Nasi
Omomyc, a Potential Myc Dominant Negative, Enhances Myc-induced Apoptosis
Cancer Res., June 1, 2002; 62(12): 3507 - 3510.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Calomme, T. L.-A. Nguyen, Y. de Launoit, V. Kiermer, L. Droogmans, A. Burny, and C. Van Lint
Upstream Stimulatory Factors Binding to an E Box Motif in the R Region of the Bovine Leukemia Virus Long Terminal Repeat Stimulates Viral Gene Expression
J. Biol. Chem., March 8, 2002; 277(11): 8775 - 8789.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Park, M. A. Wood, and M. D. Cole
BAF53 Forms Distinct Nuclear Complexes and Functions as a Critical c-Myc-Interacting Nuclear Cofactor for Oncogenic Transformation
Mol. Cell. Biol., March 1, 2002; 22(5): 1307 - 1316.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S.-G. Cho, A. Bhoumik, L. Broday, V. Ivanov, B. Rosenstein, and Z.'e. Ronai
TIP49b, a Regulator of Activating Transcription Factor 2 Response to Stress and DNA Damage
Mol. Cell. Biol., December 15, 2001; 21(24): 8398 - 8413.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
H. Carlson, S. Ota, C. E. Campbell, and P. J. Hurlin
A dominant repression domain in Tbx3 mediates transcriptional repression and cell immortalization: relevance to mutations in Tbx3 that cause ulnar-mammary syndrome
Hum. Mol. Genet., October 1, 2001; 10(21): 2403 - 2413.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
R. N. Eisenman
Deconstructing Myc
Genes & Dev., August 15, 2001; 15(16): 2023 - 2030.
[Full Text] [PDF]

Home page
 Genes Dev.Home page
C. Bouchard, O. Dittrich, A. Kiermaier, K. Dohmann, A. Menkel, M. Eilers, and B. Luscher
Regulation of cyclin D2 gene expression by the Myc/Max/Mad network: Myc-dependent TRRAP recruitment and histone acetylation at the cyclin D2 promoter
Genes & Dev., August 15, 2001; 15(16): 2042 - 2047.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
C. Merezak, C. Pierreux, E. Adam, F. Lemaigre, G. G. Rousseau, C. Calomme, C. Van Lint, D. Christophe, P. Kerkhofs, A. Burny, et al.
Suboptimal Enhancer Sequences Are Required for Efficient Bovine Leukemia Virus Propagation In Vivo: Implications for Viral Latency
J. Virol., August 1, 2001; 75(15): 6977 - 6988.
[Abstract] [Full Text]

Home page
 Toxicol SciHome page
S. E. Heid, M. K. Walker, and H. I. Swanson
Correlation of Cardiotoxicity Mediated by Halogenated Aromatic Hydrocarbons to Aryl Hydrocarbon Receptor Activation
Toxicol. Sci., May 1, 2001; 61(1): 187 - 196.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. A. Baudino and J. L. Cleveland
The Max Network Gone Mad
Mol. Cell. Biol., February 1, 2001; 21(3): 691 - 702.
[Full Text]

Home page
 Cancer Res.Home page
C. Günes, S. Lichtsteiner, A. P. Vasserot, and C. Englert
Expression of the hTERT Gene Is Regulated at the Level of Transcriptional Initiation and Repressed by Mad1
Cancer Res., April 1, 2000; 60(8): 2116 - 2121.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
S. Gehring, S. Rottmann, A. R. Menkel, J. Mertsching, A. Krippner-Heidenreich, and B. Luscher
Inhibition of Proliferation and Apoptosis by the Transcriptional Repressor Mad1. REPRESSION OF Fas-INDUCED CASPASE-8 ACTIVATION
J. Biol. Chem., March 31, 2000; 275(14): 10413 - 10420.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Y. Hou, S.-Y. Wu, T. Zhou, M. C. Thomas, and C.-M. Chiang
Alleviation of Human Papillomavirus E2-Mediated Transcriptional Repression via Formation of a TATA Binding Protein (or TFIID)-TFIIB-RNA Polymerase II-TFIIF Preinitiation Complex
Mol. Cell. Biol., January 1, 2000; 20(1): 113 - 125.
[Abstract] [Full Text]

Home page
 Mol. Pharmacol.Home page
S. E. Heid, R. S. Pollenz, and H. I. Swanson
Role of Heat Shock Protein 90 Dissociation in Mediating Agonist-Induced Activation of the Aryl Hydrocarbon Receptor
Mol. Pharmacol., January 1, 2000; 57(1): 82 - 92.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
H. Shimano, N. Yahagi, M. Amemiya-Kudo, A. H. Hasty, J.-i. Osuga, Y. Tamura, F. Shionoiri, Y. Iizuka, K. Ohashi, K. Harada, et al.
Sterol Regulatory Element-binding Protein-1 as a Key Transcription Factor for Nutritional Induction of Lipogenic Enzyme Genes
J. Biol. Chem., December 10, 1999; 274(50): 35832 - 35839.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. E. Boyd and P. J. Farnham
Coexamination of Site-Specific Transcription Factor Binding and Promoter Activity in Living Cells
Mol. Cell. Biol., December 1, 1999; 19(12): 8393 - 8399.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. V. Dang
c-Myc Target Genes Involved in Cell Growth, Apoptosis, and Metabolism
Mol. Cell. Biol., January 1, 1999; 19(1): 1 - 11.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Chytil, B. R. Peterson, D. A. Erlanson, and G. L. Verdine
The orientation of the AP-1 heterodimer on DNA strongly affects transcriptional potency
PNAS, November 24, 1998; 95(24): 14076 - 14081.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C.-T. Sun, W.-Y. Lo, I.-H. Wang, Y.-H. Lo, S.-R. Shiou, C.-K. Lai, and L.-P. Ting
Transcription Repression of Human Hepatitis B Virus Genes by Negative Regulatory Element-binding Protein/SON
J. Biol. Chem., June 22, 2001; 276(26): 24059 - 24067.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1998 by the American Society for Biochemistry and Molecular Biology.