| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 12, 7127-7133, March 20, 1998
From the Department of Biochemistry and Molecular Biology,
University of Kansas Medical Center, Kansas City, Kansas 66160-7421 and
the ¶ Department of Biological Sciences, University of Calgary,
Calgary, Alberta T2N 1N4, Canada
We examined the DNA binding activity
of mouse and human MTF-1 in whole cell extracts from cells cultured in
medium containing zinc or cadmium and from untreated cells after the
in vitro addition of zinc or cadmium, as well as using
recombinant MTF-1 transcribed and translated in vitro and
treated with various transition metals. Incubation of human (HeLa) or
mouse (Hepa) cells in medium containing cadmium (5-15
µM) did not lead to a significant increase (<2-fold) in
the amount of MTF-1 DNA binding activity, whereas zinc (100 µM) led to a 6-15-fold increase within 1 h. MTF-1
binding activity was low, but detectable, in control whole cell
extracts and was increased (>10-fold) after the in vitro
addition of zinc (30 µM) and incubation at 37 °C for
15 min. In contrast, addition of cadmium (6 or 60 µM) did
not activate MTF-1 binding activity. Recombinant mouse and human MTF-1
were also dependent on exogenous zinc for DNA binding activity. Cadmium
did not facilitate activation of recombinant MTF-1, but instead
inhibited the activation of the recombinant protein by zinc.
Interestingly, glutathione (1 mM) protected recombinant
MTF-1 from inactivation by cadmium, and allowed for activation by zinc.
It was also noted that zinc-activated recombinant MTF-1 was protected
from cadmium only when bound to DNA. These results suggest that cadmium
interacts with the zinc fingers of MTF-1 and forms an inactive complex.
Of the several transition metals (zinc, cadmium, nickel, silver,
copper, and cobalt) examined, only zinc facilitated activation of the
DNA binding activity of recombinant MTF-1. These data suggest that transition metals, other than zinc, that activate MT gene expression may do so by mechanisms independent of an increase in the DNA binding
activity of MTF-1.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Li, T. Kimura, R. W. Huyck, J. H. Laity, and G. K. Andrews Zinc-Induced Formation of a Coactivator Complex Containing the Zinc-Sensing Transcription Factor MTF-1, p300/CBP, and Sp1 Mol. Cell. Biol., July 1, 2008; 28(13): 4275 - 4284. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kobayashi, J. Kuroda, N. Shibata, T. Hasegawa, Y. Seko, M. Satoh, C. Tohyama, H. Takano, N. Imura, K. Sakabe, et al. Induction of Metallothionein by Manganese Is Completely Dependent on Interleukin-6 Production J. Pharmacol. Exp. Ther., February 1, 2007; 320(2): 721 - 727. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Yang, S. H. Kroft, and C. R. Chitambar Gene expression analysis of gallium-resistant and gallium-sensitive lymphoma cells reveals a role for metal-responsive transcription factor-1, metallothionein-2A, and zinc transporter-1 in modulating the antineoplastic activity of gallium nitrate Mol. Cancer Ther., February 1, 2007; 6(2): 633 - 643. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Li, T. Kimura, J. H. Laity, and G. K. Andrews The Zinc-Sensing Mechanism of Mouse MTF-1 Involves Linker Peptides between the Zinc Fingers Mol. Cell. Biol., August 1, 2006; 26(15): 5580 - 5587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Egli, H. Yepiskoposyan, A. Selvaraj, K. Balamurugan, R. Rajaram, A. Simons, G. Multhaup, S. Mettler, A. Vardanyan, O. Georgiev, et al. A family knockout of all four Drosophila metallothioneins reveals a central role in copper homeostasis and detoxification. Mol. Cell. Biol., March 1, 2006; 26(6): 2286 - 2296. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. M. Potter, L. S. Feng, P. Parasuram, V. A. Matskevich, J. A. Wilson, G. K. Andrews, and J. H. Laity The Six Zinc Fingers of Metal-responsive Element Binding Transcription Factor-1 Form Stable and Quasi-ordered Structures with Relatively Small Differences in Zinc Affinities J. Biol. Chem., August 5, 2005; 280(31): 28529 - 28540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Chen, B. Zhang, P. M. Harmon, W. Schaffner, D. O. Peterson, and D. P. Giedroc A Novel Cysteine Cluster in Human Metal-responsive Transcription Factor 1 Is Required for Heavy Metal-induced Transcriptional Activation in Vivo J. Biol. Chem., February 6, 2004; 279(6): 4515 - 4522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Zhang, O. Georgiev, M. Hagmann, C. Gunes, M. Cramer, P. Faller, M. Vasak, and W. Schaffner Activity of Metal-Responsive Transcription Factor 1 by Toxic Heavy Metals and H2O2 In Vitro Is Modulated by Metallothionein Mol. Cell. Biol., December 1, 2003; 23(23): 8471 - 8485. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Jiang, P. J. Daniels, and G. K. Andrews Putative Zinc-sensing Zinc Fingers of Metal-response Element-binding Transcription Factor-1 Stabilize a Metal-dependent Chromatin Complex on the Endogenous Metallothionein-I Promoter J. Biol. Chem., August 8, 2003; 278(32): 30394 - 30402. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Daniels, D. Bittel, I. V. Smirnova, D. R. Winge, and G. K. Andrews Mammalian metal response element-binding transcription factor-1 functions as a zinc sensor in yeast, but not as a sensor of cadmium or oxidative stress Nucleic Acids Res., July 15, 2002; 30(14): 3130 - 3140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Saydam, T. K. Adams, F. Steiner, W. Schaffner, and J. H. Freedman Regulation of Metallothionein Transcription by the Metal-responsive Transcription Factor MTF-1. IDENTIFICATION OF SIGNAL TRANSDUCTION CASCADES THAT CONTROL METAL-INDUCIBLE TRANSCRIPTION J. Biol. Chem., May 31, 2002; 277(23): 20438 - 20445. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. P. Giedroc, X. Chen, M. A. Pennella, and A. C. LiWang Conformational Heterogeneity in the C-terminal Zinc Fingers of Human MTF-1. AN NMR AND ZINC-BINDING STUDY J. Biol. Chem., November 2, 2001; 276(45): 42322 - 42332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Zhang, D. Egli, O. Georgiev, and W. Schaffner The Drosophila Homolog of Mammalian Zinc Finger Factor MTF-1 Activates Transcription in Response to Heavy Metals Mol. Cell. Biol., July 15, 2001; 21(14): 4505 - 4514. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. R. Davis and R. J. Cousins Metallothionein Expression in Animals: A Physiological Perspective on Function J. Nutr., May 1, 2000; 130(5): 1085 - 1088. [Abstract] [Full Text]
|
|
|
|
|
|
I. V. Smirnova, D. C. Bittel, R. Ravindra, H. Jiang, and G. K. Andrews Zinc and Cadmium Can Promote Rapid Nuclear Translocation of Metal Response Element-binding Transcription Factor-1 J. Biol. Chem., March 24, 2000; 275(13): 9377 - 9384. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Meplan, K. Mann, and P. Hainaut Cadmium Induces Conformational Modifications of Wild-type p53 and Suppresses p53 Response to DNA Damage in Cultured Cells J. Biol. Chem., October 29, 1999; 274(44): 31663 - 31670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. K. Andrews and J. Geiser Expression of the Mouse Metallothionein-I and -II Genes Provides a Reproductive Advantage during Maternal Dietary Zinc Deficiency J. Nutr., September 1, 1999; 129(9): 1643 - 1648. [Abstract] [Full Text]
|
|
|
|
 |
|
 B. J. Murphy, G. K. Andrews, D. Bittel, D. J. Discher, J. McCue, C. J. Green, M. Yanovsky, A. Giaccia, R. M. Sutherland, K. R. Laderoute, et al. Activation of Metallothionein Gene Expression by Hypoxia Involves Metal Response Elements and Metal Transcription Factor-1 Cancer Res., March 1, 1999; 59(6): 1315 - 1322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. A. Chu, J. D. Moehlenkamp, D. Bittel, G. K. Andrews, and J. A. Johnson Cadmium-mediated Activation of the Metal Response Element in Human Neuroblastoma Cells Lacking Functional Metal Response Element-binding Transcription Factor-1 J. Biol. Chem., February 26, 1999; 274(9): 5279 - 5284. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ghoshal, Y. Wang, J. F. Sheridan, and S. T. Jacob Metallothionein Induction in Response to Restraint Stress. TRANSCRIPTIONAL CONTROL, ADAPTATION TO STRESS, AND ROLE OF GLUCOCORTICOID J. Biol. Chem., October 23, 1998; 273(43): 27904 - 27910. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ghoshal, Z. Li, and S. T. Jacob Overexpression of the large subunit of the protein Ku suppresses metallothionein-I induction by heavy metals PNAS, September 1, 1998; 95(18): 10390 - 10395. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Bittel, I. V. Smirnova, and G. K. Andrews Functional Heterogeneity in the Zinc Fingers of Metalloregulatory Protein Metal Response Element-binding Transcription Factor-1 J. Biol. Chem., November 17, 2000; 275(47): 37194 - 37201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Langmade, R. Ravindra, P. J. Daniels, and G. K. Andrews The Transcription Factor MTF-1 Mediates Metal Regulation of the Mouse ZnT1 Gene J. Biol. Chem., October 27, 2000; 275(44): 34803 - 34809. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Saydam, O. Georgiev, M. Y. Nakano, U. F. Greber, and W. Schaffner Nucleo-cytoplasmic Trafficking of Metal-regulatory Transcription Factor 1 Is Regulated by Diverse Stress Signals J. Biol. Chem., June 29, 2001; 276(27): 25487 - 25495. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
