|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 13, 7488-7494, March 27, 1998
From the The zona pellucida surrounding the pig oocyte
contains two Mr 55,000 glycoproteins, pZPB and
pZPC, which are orthologues of mouse zona proteins ZP1 and ZP3,
respectively. We previously reported that isolated boar sperm membrane
vesicles possess high affinity binding sites for partially purified
pZPB, but not pZPC. Interestingly, co-incubation experiments also
implicated pZPB-pZPC complexes as potential ligands. We now report that
when depleted of a minor pZPC contaminant by size exclusion
chromatography, pZPB lacks independent binding activity. In solid phase
binding assays employing immobilized boar sperm membranes, pZPB failed
to compete with biotin-(pZPB+pZPC) probe, and biotin-labeled pZPB
yielded negligible binding. However, when co-incubated with pZPC prior
to the binding assays, pZPB acted as a potent competitor, and
biotin-labeled pZPB exhibited high affinity, saturable binding. Binding
activity was attributed to pZPB-pZPC heterocomplexes, which were
detected in co-incubation mixtures by size exclusion chromatography and Western blot analysis. In the pig, therefore, sperm membranes possess a
zona-binding protein with high affinity sites for pZPB-pZPC heterocomplexes, but not free glycoprotein subunits. Consequently, associative interactions between zona molecules can contribute toward
both the assembly of the zona matrix and generation of ligands
important for sperm-zona interactions.
Hetero-oligomerization-dependent Binding of Pig
Oocyte Zona Pellucida Glycoproteins ZPB and ZPC to Boar Sperm
Membrane Vesicles
,,
, and
Department of Obstetrics & Gynecology, Wayne
State University, Detroit, Michigan 48201, the ¶ Gamete Antigen
Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New
Delhi, India, and the Department of Biochemistry, Michigan State
University, East Lansing, Michigan 48824
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  G. Goudet, S. Mugnier, I. Callebaut, and P. Monget Phylogenetic Analysis and Identification of Pseudogenes Reveal a Progressive Loss of Zona Pellucida Genes During Evolution of Vertebrates Biol Reprod, May 1, 2008; 78(5): 796 - 806. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. van Gestel, I. A. Brewis, P. R. Ashton, J. F. Brouwers, and B. M. Gadella Multiple proteins present in purified porcine sperm apical plasma membranes interact with the zona pellucida of the oocyte Mol. Hum. Reprod., July 1, 2007; 13(7): 445 - 454. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Hasegawa, N. Kanazawa, H. Sawai, S. Komori, and K. Koyama Pig zona pellucida 2 (pZP2) protein does not participate in zona pellucida formation in transgenic mice. Reproduction, September 1, 2006; 132(3): 455 - 464. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Caballero-Campo, M. Chirinos, X. J. Fan, M. E. Gonzalez-Gonzalez, M. Galicia-Chavarria, F. Larrea, and G. L. Gerton Biological Effects of Recombinant Human Zona Pellucida Proteins on Sperm Function Biol Reprod, April 1, 2006; 74(4): 760 - 768. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Yonezawa, K. Kudo, H. Terauchi, S. Kanai, N. Yoda, M. Tanokura, K. Ito, K.-i. Miura, T. Katsumata, and M. Nakano Recombinant Porcine Zona Pellucida Glycoproteins Expressed in Sf9 Cells Bind to Bovine Sperm but Not to Porcine Sperm J. Biol. Chem., May 27, 2005; 280(21): 20189 - 20196. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. von Witzendorff, M. Ekhlasi-Hundrieser, Z. Dostalova, M. Resch, D. Rath, H.-W. Michelmann, and E. Topfer-Petersen Analysis of N-linked glycans of porcine zona pellucida glycoprotein ZPA by MALDI-TOF MS: a contribution to understanding zona pellucida structure Glycobiology, May 1, 2005; 15(5): 475 - 488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chakravarty, K. Suraj, and S. K. Gupta Baculovirus-expressed recombinant human zona pellucida glycoprotein-B induces acrosomal exocytosis in capacitated spermatozoa in addition to zona pellucida glycoprotein-C Mol. Hum. Reprod., May 1, 2005; 11(5): 365 - 372. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.J. Conner, L. Lefievre, D.C. Hughes, and C.L.R. Barratt Cracking the egg: increased complexity in the zona pellucida Hum. Reprod., May 1, 2005; 20(5): 1148 - 1152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Bausek, H. H. Ruckenbauer, S. Pfeifer, W. J. Schneider, and F. Wohlrab Interaction of Sperm with Purified Native Chicken ZP1 and ZPC Proteins Biol Reprod, August 1, 2004; 71(2): 684 - 690. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Lefievre, S.J. Conner, A. Salpekar, O. Olufowobi, P. Ashton, B. Pavlovic, W. Lenton, M. Afnan, I.A. Brewis, M. Monk, et al. Four zona pellucida glycoproteins are expressed in the human Hum. Reprod., July 1, 2004; 19(7): 1580 - 1586. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. H. Vo, T.-Y. Yen, B. A. Macher, and J. L. Hedrick Identification of the ZPC Oligosaccharide Ligand Involved in Sperm Binding and the Glycan Structures of Xenopus laevis Vitelline Envelope Glycoproteins Biol Reprod, December 1, 2003; 69(6): 1822 - 1830. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hemachand, B. Gopalakrishnan, D. M. Salunke, S. M. Totey, and C. Shaha Sperm plasma-membrane-associated glutathione S-transferases as gamete recognition molecules J. Cell Sci., May 15, 2002; 115(10): 2053 - 2065. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Miller, X. Shi, and H. Burkin Molecular Basis of Mammalian Gamete Binding Recent Prog. Horm. Res., January 1, 2002; 57(1): 37 - 73. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Baker, R. A. Cardullo, and C. D. Thaler Sonication of Mouse Sperm Membranes Reveals Distinct Protein Domains Biol Reprod, January 1, 2002; 66(1): 57 - 64. [Abstract] [Full Text]
|
|
|
|
|
|
C. D. Thaler and R. A. Cardullo Distinct Membrane Fractions from Mouse Sperm Bind Different Zona Pellucida Glycoproteins Biol Reprod, January 1, 2002; 66(1): 65 - 69. [Abstract] [Full Text]
|
|
|
|
|
|
B. S. Dunbar, T. M. Timmons, S. M. Skinner, and S. V. Prasad Molecular Analysis of a Carbohydrate Antigen Involved in the Structure and Function of Zona Pellucida Glycoproteins Biol Reprod, September 1, 2001; 65(3): 951 - 960. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. K. Govind, G. K. Gahlay, S. Choudhury, and S. K. Gupta Purified and Refolded Recombinant Bonnet Monkey (Macaca radiata) Zona Pellucida Glycoprotein-B Expressed in Escherichia coli Binds to Spermatozoa Biol Reprod, April 1, 2001; 64(4): 1147 - 1152. [Abstract] [Full Text]
|
|
|
|
|
|
C. K. Govind, A. Hasegawa, K. Koyama, and S. K. Gupta Delineation of a Conserved B Cell Epitope on Bonnet Monkey (Macaca radiata) and Human Zona Pellucida Glycoprotein-B by Monoclonal Antibodies Demonstrating Inhibition of Sperm-Egg Binding Biol Reprod, January 1, 2000; 62(1): 67 - 75. [Abstract] [Full Text]
|
|
|
|
 |
|
 T Rankin, P Talbot, E Lee, and J Dean Abnormal zonae pellucidae in mice lacking ZP1 result in early embryonic loss Development, January 9, 1999; 126(17): 3847 - 3855. [Abstract] [PDF]
|
|
|
|
|
|
T. Rankin, Z. Tong, P. Castle, E Lee, R Gore-Langton, L. Nelson, and J Dean Human ZP3 restores fertility in Zp3 null mice without affecting order-specific sperm binding Development, January 7, 1998; 125(13): 2415 - 2424. [Abstract] [PDF]
|
|
|
|
|
|
N. Bausek, M. Waclawek, W. J. Schneider, and F. Wohlrab The Major Chicken Egg Envelope Protein ZP1 Is Different from ZPB and Is Synthesized in the Liver J. Biol. Chem., September 8, 2000; 275(37): 28866 - 28872. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |