HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Shaw, G.-C. Articles by Lin, C.-H. Search for Related Content PubMed PubMed Citation Articles by Shaw, G.-C. Articles by Lin, C.-H. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 14, 7996-8002, April 3, 1998

Evidence against the Bm1P1 Protein as a Positive Transcription Factor for Barbiturate-mediated Induction of Cytochrome P450_BM-1 in Bacillus megaterium

From the Institute of Biochemistry, School of Life Science, National Yang-Ming University, Taipei 112, Taiwan, Republic of China

The Bm1P1 protein was previously proposed to act as a positive transcription factor involved in barbiturate-mediated induction of cytochrome P450_BM-1 in Bacillus megaterium. We now report that the bm1P1 gene encodes a protein of 217 amino acids, rather than the 98 amino acids as reported previously. In vitro gel shift assays indicate that the Bm1P1 protein did not interact with probes comprising the regulatory regions of the P450_BM-1 gene. Moreover, disruption of the bm1P1 gene did not markedly affect barbiturate induction of P450_BM-1 expression. A multicopy plasmid harboring only the P450_BM-1 promoter region could increase expression of the chromosome-encoded P450_BM-1. The level of expression is comparable with that shown by a multicopy plasmid harboring the P450_BM-1 promoter region along with the bm1P1 gene. These results strongly suggest that the Bm1P1 protein is unlikely to act as a positive regulator for barbiturate induction of P450_BM-1 expression. Finally, deletion of the Barbie box did not markedly diminish the effect of pentobarbital on expression of a reporter gene transcriptionally fused to the P450_BM-1 promoter. This suggests that the Barbie box is unlikely to be a key element in barbiturate-mediated induction of P450_BM-1.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				J. L. Ramos, M. Martinez-Bueno, A. J. Molina-Henares, W. Teran, K. Watanabe, X. Zhang, M. T. Gallegos, R. Brennan, and R. Tobes The TetR Family of Transcriptional Repressors Microbiol. Mol. Biol. Rev., June 1, 2005; 69(2): 326 - 356. [Abstract] [Full Text] [PDF]

				C. Handschin and U. A. Meyer Induction of Drug Metabolism: The Role of Nuclear Receptors Pharmacol. Rev., December 1, 2003; 55(4): 649 - 673. [Abstract] [Full Text] [PDF]

				G.-C. Shaw, Y.-H. Hsueh, C.-C. Sung, Y.-S. Chen, and C.-H. Liu Negative Regulation of Expression of the Bacillus megaterium bmlP1 Gene by the bmlP1 3' Flanking Region J. Bacteriol., May 1, 2000; 182(9): 2654 - 2659. [Abstract] [Full Text]

				S. C. Dogra, B. P. Davidson, and B. K. May Analysis of a Phenobarbital-Responsive Enhancer Sequence Located in the 5' Flanking Region of the Chicken CYP2H1 Gene: Identification and Characterization of Functional Protein-Binding Sites Mol. Pharmacol., January 1, 1999; 55(1): 14 - 22. [Abstract] [Full Text]

				E. T. Morgan, M. B. Sewer, H. Iber, F. J. Gonzalez, Y.-H. Lee, R. H. Tukey, S. Okino, T. Vu, Y.-H. Chen, J. S. Sidhu, et al. Physiological and Pathophysiological Regulation of Cytochrome P450 Drug Metab. Dispos., December 1, 1998; 26(12): 1232 - 1240. [Abstract] [Full Text]