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J Biol Chem, Vol. 273, Issue 14, 8287-8293, April 3, 1998
From the Service de Microbiologie, The genetic expression of human B19 parvovirus is
only dependent on one promoter in vivo and in
vitro. This is the P6 promoter, which is located on the left side
of the genome and is a single-stranded DNA molecule. This led us to
investigate the regulation of the P6 promoter and the possible
resulting variability of the nucleotide sequence. After analysis of the
promoter region of 17 B19 strains, only 1.5% variability was found.
More exciting was the finding of mutations that were clustered around
the TATA box and defined a highly conserved region (nucleotides
113-210) in the proximal part of the P6 promoter. HeLa and UT7/Epo
cell extracts were found to protect this region, which contained a core
motif for Ets family proteins, with YY1 and Sp1 binding sites on either
side. Gel mobility shift assays performed with nuclear proteins from
HeLa and UT7/Epo cells identified DNA-binding proteins specific for
these sites. By supershift analysis, we demonstrated the binding of the
hGABP (also named E4TF1) protein to the Ets binding site and the
fixation of Sp1 and YY1 proteins on their respective motifs. In
Drosophila SL2 cells, hGABP
Regulation of Human B19 Parvovirus Promoter Expression by hGABP
(E4TF1) Transcription Factor
,,, and
Unité de
Virologie and CNRS UPR 9051, Hôpital Saint-Louis, 1 avenue
Claude Vellefaux, 75475 Paris CEDEX 10, France, § INSERM
U380, Institut Cochin de Génétique Moléculaire, 22 rue Méchain, 75014 Paris, France, and ¶ Faculty of
Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsu-cho, Midori-ku, Yokohama 226, Japan
and -
stimulated P6
promoter activity, and hGABP/hGABP and Sp1 exerted synergistic
stimulation of this activity, an effect diminished by YY1.
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
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