HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Heinrichs, D. E. Articles by Whitfield, C. Search for Related Content PubMed PubMed Citation Articles by Heinrichs, D. E. Articles by Whitfield, C. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 15, 8849-8859, April 10, 1998

The Assembly System for the Lipopolysaccharide R2 Core-type of Escherichia coli Is a Hybrid of Those Found in Escherichia coli K-12 and Salmonella enterica
STRUCTURE AND FUNCTION OF THE R2 WaaK AND WaaL HOMOLOGS

David E. Heinrichs, Mario A. Monteiro^¶, Malcolm B. Perry^¶, and Chris Whitfield

From the  Department of Microbiology, University of Guelph, Guelph, Ontario, Canada N1G 2W1 and ^¶ Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada K1A OR6

In Escherichia coli F632, the 14-kilobase pair chromosomal region located between waaC (formerly rfaC) and waaA (kdtA) contains genes encoding enzymes required for the synthesis of the type R2 core oligosaccharide portion of lipopolysaccharide. Ten of the 13 open reading frames encode predicted products sharing greater than 90% total similarity with homologs in E. coli K-12. However, the products of waaK (rfaK) and waaL (rfaL) each resemble homologs in Salmonella enterica serovar Typhimurium but share little similarity with E. coli K-12. The F632 WaaK and WaaL proteins therefore define differences between the type R2 and K-12 outer core oligosaccharides of E. coli lipopolysaccharides. Based on the chemical structure of the core oligosaccharide of an E. coli F632 waaK::aacC1 mutant and in vitro glycosyltransferase analyses, waaK encodes UDP-N-acetylglucosamine:(glucose) lipopolysaccharide 1,2-N-acetylglucosaminyltransferase. The WaaK enzyme adds a terminal GlcNAc side branch substituent that is crucial for the recognition of core oligosaccharide acceptor by the O-polysaccharide ligase, WaaL. Results of complementation analyses of E. coli K-12 and F632 waaL mutants suggest that structural differences between the WaaL proteins play a role in recognition of, and interaction with, terminal lipopolysaccharide core moieties.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				M. Rangarajan, J. Aduse-Opoku, N. Paramonov, A. Hashim, N. Bostanci, O. P. Fraser, E. Tarelli, and M. A. Curtis Identification of a Second Lipopolysaccharide in Porphyromonas gingivalis W50 J. Bacteriol., April 15, 2008; 190(8): 2920 - 2932. [Abstract] [Full Text] [PDF]

				A. Nakhamchik, C. Wilde, and D. A. Rowe-Magnus Identification of a Wzy Polymerase Required for Group IV Capsular Polysaccharide and Lipopolysaccharide Biosynthesis in Vibrio vulnificus Infect. Immun., December 1, 2007; 75(12): 5550 - 5558. [Abstract] [Full Text] [PDF]

				M. D. Leipold, E. Vinogradov, and C. Whitfield Glycosyltransferases Involved in Biosynthesis of the Outer Core Region of Escherichia coli Lipopolysaccharides Exhibit Broader Substrate Specificities Than Is Predicted from Lipopolysaccharide Structures J. Biol. Chem., September 14, 2007; 282(37): 26786 - 26792. [Abstract] [Full Text] [PDF]

				K. M. Pepin, M. A. Samuel, and H. A. Wichman Variable Pleiotropic Effects From Mutations at the Same Locus Hamper Prediction of Fitness From a Fitness Component Genetics, April 1, 2006; 172(4): 2047 - 2056. [Abstract] [Full Text] [PDF]

				A. Hoare, M. Bittner, J. Carter, S. Alvarez, M. Zaldivar, D. Bravo, M. A. Valvano, and I. Contreras The Outer Core Lipopolysaccharide of Salmonella enterica Serovar Typhi Is Required for Bacterial Entry into Epithelial Cells Infect. Immun., March 1, 2006; 74(3): 1555 - 1564. [Abstract] [Full Text] [PDF]

				R. Langdon, J. E Craig, M. Goldrick, R. Houldsworth, and N. J High Analysis of the role of HP0208, a phase-variable open reading frame, and its homologues HP1416 and HP0159 in the biosynthesis of Helicobacter pylori lipopolysaccharide J. Med. Microbiol., August 1, 2005; 54(8): 697 - 706. [Abstract] [Full Text] [PDF]

				S. Schild, A.-K. Lamprecht, and J. Reidl Molecular and Functional Characterization of O Antigen Transfer in Vibrio cholerae J. Biol. Chem., July 8, 2005; 280(27): 25936 - 25947. [Abstract] [Full Text] [PDF]

				G. Li, C. Laturnus, C. Ewers, and L. H. Wieler Identification of Genes Required for Avian Escherichia coli Septicemia by Signature-Tagged Mutagenesis Infect. Immun., May 1, 2005; 73(5): 2818 - 2827. [Abstract] [Full Text] [PDF]

				P. D. Abeyrathne, C. Daniels, K. K. H. Poon, M. J. Matewish, and J. S. Lam Functional Characterization of WaaL, a Ligase Associated with Linking O-Antigen Polysaccharide to the Core of Pseudomonas aeruginosa Lipopolysaccharide J. Bacteriol., May 1, 2005; 187(9): 3002 - 3012. [Abstract] [Full Text] [PDF]

				N. A. Kaniuk, E. Vinogradov, and C. Whitfield Investigation of the Structural Requirements in the Lipopolysaccharide Core Acceptor for Ligation of O Antigens in the Genus Salmonella: WAAL "LIGASE" IS NOT THE SOLE DETERMINANT OF ACCEPTOR SPECIFICITY J. Biol. Chem., August 27, 2004; 279(35): 36470 - 36480. [Abstract] [Full Text] [PDF]

				N. A. Kaniuk, E. Vinogradov, J. Li, M. A. Monteiro, and C. Whitfield Chromosomal and Plasmid-encoded Enzymes Are Required for Assembly of the R3-type Core Oligosaccharide in the Lipopolysaccharide of Escherichia coli O157:H7 J. Biol. Chem., July 23, 2004; 279(30): 31237 - 31250. [Abstract] [Full Text] [PDF]

				E. Frirdich, E. Vinogradov, and C. Whitfield Biosynthesis of a Novel 3-Deoxy-D-manno-oct-2-ulosonic Acid-containing Outer Core Oligosaccharide in the Lipopolysaccharide of Klebsiella pneumoniae J. Biol. Chem., July 2, 2004; 279(27): 27928 - 27940. [Abstract] [Full Text] [PDF]

				R. Novotny, C. Schaffer, J. Strauss, and P. Messner S-layer glycan-specific loci on the chromosome of Geobacillus stearothermophilus NRS 2004/3a and dTDP-L-rhamnose biosynthesis potential of G. stearothermophilus strains Microbiology, April 1, 2004; 150(4): 953 - 965. [Abstract] [Full Text] [PDF]

				N. Coderch, N. Pique, B. Lindner, N. Abitiu, S. Merino, L. Izquierdo, N. Jimenez, J. M. Tomas, O. Holst, and M. Regue Genetic and Structural Characterization of the Core Region of the Lipopolysaccharide from Serratia marcescens N28b (Serovar O4) J. Bacteriol., February 15, 2004; 186(4): 978 - 988. [Abstract] [Full Text] [PDF]

				C. Longman, M. Brockington, S. Torelli, C. Jimenez-Mallebrera, C. Kennedy, N. Khalil, L. Feng, R. K. Saran, T. Voit, L. Merlini, et al. Mutations in the human LARGE gene cause MDC1D, a novel form of congenital muscular dystrophy with severe mental retardation and abnormal glycosylation of {alpha}-dystroglycan Hum. Mol. Genet., November 1, 2003; 12(21): 2853 - 2861. [Abstract] [Full Text] [PDF]

				P. K. Grewal and J. E. Hewitt Glycosylation defects: a new mechanism for muscular dystrophy? Hum. Mol. Genet., October 15, 2003; 12(90002): R259 - 264. [Abstract] [Full Text] [PDF]

				L. Goodridge, A. Gallaccio, and M. W. Griffiths Morphological, Host Range, and Genetic Characterization of Two Coliphages Appl. Envir. Microbiol., September 1, 2003; 69(9): 5364 - 5371. [Abstract] [Full Text] [PDF]

				E. Frirdich, B. Lindner, O. Holst, and C. Whitfield Overexpression of the waaZ Gene Leads to Modification of the Structure of the Inner Core Region of Escherichia coli Lipopolysaccharide, Truncation of the Outer Core, and Reduction of the Amount of O Polysaccharide on the Cell Surface J. Bacteriol., March 1, 2003; 185(5): 1659 - 1671. [Abstract] [Full Text] [PDF]

				D.-Q. Xu, J. O. Cisar, N. Ambulos Jr., D. H. Burr, and D. J. Kopecko Molecular Cloning and Characterization of Genes for Shigella sonnei Form I O Polysaccharide: Proposed Biosynthetic Pathway and Stable Expression in a Live Salmonella Vaccine Vector Infect. Immun., August 1, 2002; 70(8): 4414 - 4423. [Abstract] [Full Text] [PDF]

				M. Regué, N. Climent, N. Abitiu, N. Coderch, S. Merino, L. Izquierdo, M. Altarriba, and J. M. Tomás Genetic Characterization of the Klebsiella pneumoniae waa Gene Cluster, Involved in Core Lipopolysaccharide Biosynthesis J. Bacteriol., June 15, 2001; 183(12): 3564 - 3573. [Abstract] [Full Text]

				M. McClelland, L. Florea, K. Sanderson, S. W. Clifton, J. Parkhill, C. Churcher, G. Dougan, R. K. Wilson, and W. Miller Comparison of the Escherichia coli K-12 genome with sampled genomes of a Klebsiella pneumoniae and three Salmonella enterica serovars, Typhimurium, Typhi and Paratyphi Nucleic Acids Res., December 15, 2000; 28(24): 4974 - 4986. [Abstract] [Full Text] [PDF]

				J. G. Shepherd, L. Wang, and P. R. Reeves Comparison of O-Antigen Gene Clusters of Escherichia coli (Shigella) Sonnei and Plesiomonas shigelloides O17: Sonnei Gained Its Current Plasmid-Borne O-Antigen Genes from P. shigelloides in a Recent Event Infect. Immun., October 1, 2000; 68(10): 6056 - 6061. [Abstract] [Full Text] [PDF]

				M. T. Sebulsky, D. Hohnstein, M. D. Hunter, and D. E. Heinrichs Identification and Characterization of a Membrane Permease Involved in Iron-Hydroxamate Transport in Staphylococcus aureus J. Bacteriol., August 15, 2000; 182(16): 4394 - 4400. [Abstract] [Full Text]

				K. Amor, D. E. Heinrichs, E. Frirdich, K. Ziebell, R. P. Johnson, and C. Whitfield Distribution of Core Oligosaccharide Types in Lipopolysaccharides from Escherichia coli Infect. Immun., March 1, 2000; 68(3): 1116 - 1124. [Abstract] [Full Text] [PDF]

				C. Whitfield, D. E. Heinrichs, J. A. Yethon, K. L. Amor, M. A. Monteiro, and M. B. Perry Assembly of the R1-type core oligosaccharide of Escherichia coli lipopolysaccharide Innate Immunity, June 1, 1999; 5(3): 151 - 156. [Abstract] [PDF]

				D. E. Heinrichs, J. A. Yethon, P. A. Amor, and C. Whitfield The Assembly System for the Outer Core Portion of R1- and R4-type Lipopolysaccharides of Escherichia coli. THE R1 CORE-SPECIFIC beta -GLUCOSYLTRANSFERASE PROVIDES A NOVEL ATTACHMENT SITE FOR O-POLYSACCHARIDES J. Biol. Chem., November 6, 1998; 273(45): 29497 - 29505. [Abstract] [Full Text] [PDF]

				K. Shibayama, S. Ohsuka, T. Tanaka, Y. Arakawa, and M. Ohta Conserved Structural Regions Involved in the Catalytic Mechanism of Escherichia coli K-12 WaaO (RfaI) J. Bacteriol., October 15, 1998; 180(20): 5313 - 5318. [Abstract] [Full Text]

				M. K. B. Berlyn Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map Microbiol. Mol. Biol. Rev., September 1, 1998; 62(3): 814 - 984. [Abstract] [Full Text] [PDF]