![]()
|
|
||||||||
J Biol Chem, Vol. 273, Issue 16, 10019-10025, April 17, 1998
From the Department of Biochemistry, University of Wisconsin,
Madison, Wisconsin 53706
CooJ, a nickel-binding protein from the CO
dehydrogenase system of Rhodospirillum rubrum, was purified
by immobilized metal affinity chromatography. CooJ is a CO-induced
protein predicted to contain a nickel binding motif composed of 16 histidine residues in the final 34 amino acids of the 12.5-kDa protein.
When cells grown in the presence of CO were fractionated on an
immobilized metal affinity chromatography column and analyzed by
SDS-polyacrylamide gel electrophoresis, the major protein observed in
the effluent migrated at an apparent molecular mass of 19 kDa. The
19-kDa protein was absent in extracts of cells grown in the absence of
CO and the mutant strain, UR294, which lacks a functional
cooJ gene. N-terminal sequence analysis confirmed that the
19-kDa protein is the product of the cooJ gene. Purified
CooJ was shown to bind four nickel atoms per CooJ monomer with a
Kd of 4.3 µM. Other divalent metals
competed with the following order of affinity and corresponding
Ki: Zn2+ (5 µM) > Cd2+ (19 µM) > Co2+ (23 µM) > Cu2+ (122 µM). CooJ
chromatographed on a calibrated Superose 12 gel filtration column
eluted at 39 kDa, a position consistent with a multimeric native
molecular mass for CooJ.
This article has been cited by other articles:
![]() |
A. Atanassova and D. B. Zamble Escherichia coli HypA Is a Zinc Metalloprotein with a Weak Affinity for Nickel J. Bacteriol., July 15, 2005; 187(14): 4689 - 4697. [Abstract] [Full Text] [PDF] |
||||
![]() |
W. B. Jeon, J. Cheng, and P. W. Ludden Purification and Characterization of Membrane-associated CooC Protein and Its Functional Role in the Insertion of Nickel into Carbon Monoxide Dehydrogenase from Rhodospirillum rubrum J. Biol. Chem., October 12, 2001; 276(42): 38602 - 38609. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. J. Colpas and R. P. Hausinger In Vivo and in Vitro Kinetics of Metal Transfer by the Klebsiella aerogenes Urease Nickel Metallochaperone, UreE J. Biol. Chem., April 6, 2000; 275(15): 10731 - 10737. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. García-Domínguez, L. Lopez-Maury, F. J. Florencio, and J. C. Reyes A Gene Cluster Involved in Metal Homeostasis in the Cyanobacterium Synechocystis sp. Strain PCC 6803 J. Bacteriol., March 15, 2000; 182(6): 1507 - 1514. [Abstract] [Full Text] |
||||
![]() |
R. K. Watt and P. W. Ludden Ni2+ Transport and Accumulation in Rhodospirillum rubrum J. Bacteriol., August 1, 1999; 181(15): 4554 - 4560. [Abstract] [Full Text] |
||||
![]() |
A. Battistoni, F. Pacello, A. P. Mazzetti, C. Capo, J. S. Kroll, P. R. Langford, A. Sansone, G. Donnarumma, P. Valenti, and G. Rotilio A Histidine-rich Metal Binding Domain at the N Terminus of Cu,Zn-Superoxide Dismutases from Pathogenic Bacteria. A NOVEL STRATEGY FOR METAL CHAPERONING J. Biol. Chem., August 3, 2001; 276(32): 30315 - 30325. [Abstract] [Full Text] [PDF] |
||||
![]() |
H. K. Song, S. B. Mulrooney, R. Huber, and R. P. Hausinger Crystal Structure of Klebsiella aerogenes UreE, a Nickel-binding Metallochaperone for Urease Activation J. Biol. Chem., December 21, 2001; 276(52): 49359 - 49364. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |