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J Biol Chem, Vol. 273, Issue 16, 9388-9392, April 17, 1998

Heterochromatin Organization of a Natural Yeast Telomere
CHANGES OF NUCLEOSOME DISTRIBUTION DRIVEN BY THE ABSENCE OF Sir3p

Miguel Angel Vega-Palas, Sabrina Venditti^¶, and Ernesto Di Mauro^¶

From the ^¶ Fondazione Istituto Pasteur-Fondazione Cenci-Bolognetti, c/o Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza, P.le Aldo Moro 5, 00185 Roma, Italy and  Centro Acidi Nucleici, Consiglio Nazionale delle Ricerche, Roma Italy

We have defined the in vivo heterochromatin structure of the left telomere of Saccharomyces cerevisiae chromosome III (LIII). Analysis of heterochromatin of a single telomere was so far lacking, due to the difficulties intrinsic to the highly repetitive nature of telomeric sequences. In LIII, the terminal (C_1-3A)_n repetitive sequences are followed by a complete X element and by the single copy Ty5-1 retrotransposon. Both the telosome and the X element exhibit overall resistance to micrococcal nuclease digestion reflecting their tight chromatin structure organization. The X element contains protein complexes and irregularly distributed but well localized nucleosomes. In contrast, a regular array of phased nucleosomes is associated with the promoter region of Ty5-1 and with the more centromere-proximal sequences. The lack of a structural component of yeast telomeres, the SIR3 protein, does not alter the overall tight organization of the X element but causes a nucleosome rearrangement within the promoter region of Ty5-1 and releases Ty5-1 silencing. Thus, Sir3p links the modification of the heterochromatin structure with loss of transcriptional silencing.

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