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J Biol Chem, Vol. 273, Issue 16, 9630-9636, April 17, 1998

Expression of a Plastidic ATP/ADP Transporter Gene in Escherichia coli Leads to a Functional Adenine Nucleotide Transport System in the Bacterial Cytoplasmic Membrane

Joachim Tjaden, Christian Schwöppe, Torsten Möhlmann, Paul W. Quick, and H. Ekkehard Neuhaus

From the Pflanzenphysiologie, Universität Osnabrück, Barbarastr. 11, D-49069 Osnabrück, Germany and the  Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2UQ, United Kingdom

Recently, a second type of eucaryotic adenine nucleotide transporter located in the inner envelope membrane of higher plants has been identified at the molecular level (Neuhaus, H. E., Thom, E., Möhlmann, T., Steup, M., and Kampfenkel, K. (1997) Plant J. 11, 73-82). Here we have analyzed the biochemical properties of this ATP/ADP transporter from Arabidopsis thaliana (AATP1, At). This analysis was carried out by expressing a cDNA encoding this carrier as a histidine-tagged chimeric protein heterologously in Escherichia coli. Isopropyl-1-thio--D-galactopyranoside (IPTG)-induced E. coli cells were able to import radioactively labeled [-³²P]ATP. Uninduced E. coli cells did not import [-³²P]ATP. Further control experiments revealed that IPTG induction did not promote import of other phosphorylated or unphosphorylated metabolites into the bacterial cell indicating the specificity of [-³²P]ATP transport. [-³²P]ATP uptake into induced E. coli cells was linear with time for several minutes allowing for determination of kinetic constants. The apparent K_m for ATP was 17 µM which is close to values reported on the authentic protein in isolated plastids. ADP was a strong competitive inhibitor of [-³²P]ATP uptake (K_i ADP 3.6 µM). Other metabolites like AMP, ADP glucose, UTP, UDP, NAD, and NADP did not influence [-³²P]ATP uptake. IPTG-induced E. coli cells preloaded with [-³²P]ATP exported radioactively labeled adenylates after exogenous addition of unlabeled ATP or ADP indicating a counter exchange mechanism of transport. The biochemical properties of the heterologously expressed AATP1 gene product demonstrated that the protein is functionally integrated in the cytoplasmic membrane of E. coli. This is the first report of the functional expression of a plant membrane protein in E. coli leading to new transport properties across the cytoplasmic membrane. The functional integration of a plant membrane protein in the cytoplasmic membrane of E. coli offers new possibilities for future studies of the structural and mechanistic properties of this transporter. Since IPTG induction allowed synthesis of a 67-kDa protein in E. coli, which was subsequently specifically enriched by metal-chelate chromatography, this procaryotic heterologous expression system might provide a suitable system for overproduction of membrane proteins of eucaryotic origin in the near future.

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