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J Biol Chem, Vol. 273, Issue 17, 10389-10395, April 24, 1998

Synchronized Spontaneous Ca²⁺ Transients in Acute Anterior Pituitary Slices

From INSERM U469, Centre CNRS/INSERM de Pharmacologie et d'Endocrinologie, 141 rue de la Cardonille, 34094 Montpellier Cedex 5, France

We investigated the organization of spontaneous rises in cytosolic free Ca²⁺ concentration ([Ca²⁺]_i) due to electrical activity in acute pituitary slices. Real time confocal imaging revealed that 73% of the cells generated fast peaking spontaneous [Ca²⁺]_i transients. Strikingly, groups of apposing cells enhanced their [Ca²⁺]_i in synchrony with a speed of coactivation >1,000 µm/s. Single-cell injection of Neurobiotin or Lucifer yellow labeled clusters of cells, which corresponded to coactive cells. Halothane, a gap junction blocker, markedly reduced the spread of tracers. Coupling between excitable cells was mainly homologous in nature, with a prevalence of growth hormone-containing cells. We conclude that spontaneously active endocrine cells are either single units or arranged in synchronized gap junction-coupled assemblies scattered throughout the anterior pituitary gland. Synchrony between spontaneously excitable cells may help shape the patterns of basal secretion.

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