JBC Advanced Peptides, Inc.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nantel, A.
Right arrow Articles by Thomas, D. Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nantel, A.
Right arrow Articles by Thomas, D. Y.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 273, Issue 17, 10475-10484, April 24, 1998

Interaction of the Grb10 Adapter Protein with the Raf1 and MEK1 Kinases

André NantelDagger , Khosro Mohammad-Ali, Jennifer SherkDagger , Barry I. Posner, and David Y. ThomasDagger par

From the Dagger  Eukaryotic Genetics Group, Biotechnology Research Institute, National Research Council, 6100 Royalmount, Montreal, Quebec H4P 2R2, Canada, the  Department of Experimental Medicine, McGill University, Montreal, Quebec H3A 2B2, Canada, and the par  Biology Department and Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec H3A 2B2, Canada

Grb10 and its close homologues Grb7 and Grb14, belong to a family of adapter proteins characterized by a proline-rich region, a central PH domain, and a carboxyl-terminal Src homology 2 (SH2) domain. Their interaction with a variety of activated tyrosine kinase receptors is well documented, but their actual function remains a mystery. The Grb10 SH2 domain was isolated from a two-hybrid screen using the MEK1 kinase as a bait. We show that this unusual SH2 domain interacts, in a phosphotyrosine-independent manner, with both the Raf1 and MEK1 kinases. Mutation of the MEK1 Thr-386 residue, which is phosphorylated by mitogen-activated protein kinase in vitro, reduces binding to Grb10 in a two-hybrid assay. Interaction of Grb10 with Raf1 is constitutive, while interaction between Grb10 and MEK1 needs insulin treatment of the cells and follows mitogen-activated protein kinase activation. Random mutagenesis of the SH2 domain demonstrated that the Arg-beta B5 and Asp-EF2 residues are necessary for binding to the epidermal growth factor and insulin receptors as well as to the two kinases. In addition, we show that a mutation in Ser-beta B7 affects binding only to the receptors, while a mutation in Thr-beta C5 abrogates binding only to MEK1. Finally, transfection of Grb10 genes with specific mutations in their SH2 domains induces apoptosis in HTC-IR and COS-7 cells. These effects can be competed by co-expression of the wild type protein, suggesting that these mutants act by sequestering necessary signaling components.

Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S. Kebache, J. Ash, M. G. Annis, J. Hagan, M. Huber, J. Hassard, C. L. Stewart, M. Whiteway, and A. Nantel
Grb10 and Active Raf-1 Kinase Promote Bad-dependent Cell Survival
J. Biol. Chem., July 27, 2007; 282(30): 21873 - 21883.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
V. Bissonauth, S. Roy, M. Gravel, S. Guillemette, and J. Charron
Requirement for Map2k1 (Mek1) in extra-embryonic ectoderm during placentogenesis
Development, September 1, 2006; 133(17): 3429 - 3440.
[Abstract] [Full Text] [PDF]

Home page
 Protein Sci.Home page
M. Ivancic, A. M. Spuches, E. C. Guth, M. A. Daugherty, D. E. Wilcox, and B. A. Lyons
Backbone nuclear relaxation characteristics and calorimetric investigation of the human Grb7-SH2/erbB2 peptide complex
Protein Sci., June 1, 2005; 14(6): 1556 - 1569.
[Abstract] [Full Text] [PDF]

Home page
 Arch DermatolHome page
S. Nambiar, A. Mirmohammadsadegh, R. Doroudi, A. Gustrau, A. Marini, G. Roeder, T. Ruzicka, and U. R. Hengge
Signaling Networks in Cutaneous Melanoma Metastasis Identified by Complementary DNA Microarrays
Arch Dermatol, February 1, 2005; 141(2): 165 - 173.
[Abstract] [Full Text] [PDF]

Home page
 Protein Sci.Home page
P. J. Scharf, J. Witney, R. Daly, and B. A. Lyons
Solution structure of the human Grb14-SH2 domain and comparison with the structures of the human Grb7-SH2/erbB2 peptide complex and human Grb10-SH2 domain
Protein Sci., September 1, 2004; 13(9): 2541 - 2546.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Murdaca, C. Treins, M.-N. Monthouel-Kartmann, R. Pontier-Bres, S. Kumar, E. Van Obberghen, and S. Giorgetti-Peraldi
Grb10 Prevents Nedd4-mediated Vascular Endothelial Growth Factor Receptor-2 Degradation
J. Biol. Chem., June 18, 2004; 279(25): 26754 - 26761.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Deng, S. Bhattacharya, O. R. Swamy, R. Tandon, Y. Wang, R. Janda, and H. Riedel
Growth Factor Receptor-binding Protein 10 (Grb10) as a Partner of Phosphatidylinositol 3-Kinase in Metabolic Insulin Action
J. Biol. Chem., October 10, 2003; 278(41): 39311 - 39322.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Giovannone, E. Lee, L. Laviola, F. Giorgino, K. A. Cleveland, and R. J. Smith
Two Novel Proteins That Are Linked to Insulin-like Growth Factor (IGF-I) Receptors by the Grb10 Adapter and Modulate IGF-I Signaling
J. Biol. Chem., August 22, 2003; 278(34): 31564 - 31573.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
L.-F. Belanger, S. Roy, M. Tremblay, B. Brott, A.-M. Steff, W. Mourad, P. Hugo, R. Erikson, and J. Charron
Mek2 Is Dispensable for Mouse Growth and Development
Mol. Cell. Biol., July 15, 2003; 23(14): 4778 - 4787.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Charalambous, F. M. Smith, W. R. Bennett, T. E. Crew, F. Mackenzie, and A. Ward
Disruption of the imprinted Grb10 gene leads to disproportionate overgrowth by an Igf2-independent mechanism
PNAS, July 8, 2003; 100(14): 8292 - 8297.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Vecchione, A. Marchese, P. Henry, D. Rotin, and A. Morrione
The Grb10/Nedd4 Complex Regulates Ligand-Induced Ubiquitination and Stability of the Insulin-Like Growth Factor I Receptor
Mol. Cell. Biol., May 1, 2003; 23(9): 3363 - 3372.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Kast, M. Wang, and M. Whiteway
The ERK/MAPK Pathway Regulates the Activity of the Human Tissue Factor Pathway Inhibitor-2 Promoter
J. Biol. Chem., February 21, 2003; 278(9): 6787 - 6794.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
B. Cariou, D. Perdereau, K. Cailliau, E. Browaeys-Poly, V. Bereziat, M. Vasseur-Cognet, J. Girard, and A.-F. Burnol
The Adapter Protein ZIP Binds Grb14 and Regulates Its Inhibitory Action on Insulin Signaling by Recruiting Protein Kinase C{zeta}
Mol. Cell. Biol., October 15, 2002; 22(20): 6959 - 6970.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. Jahn, P. Seipel, S. Urschel, C. Peschel, and J. Duyster
Role for the Adaptor Protein Grb10 in the Activation of Akt
Mol. Cell. Biol., February 15, 2002; 22(4): 979 - 991.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Bereziat, A. Kasus-Jacobi, D. Perdereau, B. Cariou, J. Girard, and A.-F. Burnol
Inhibition of Insulin Receptor Catalytic Activity by the Molecular Adapter Grb14
J. Biol. Chem., February 8, 2002; 277(7): 4845 - 4852.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Cha, E. K. Lee, and P. Shapiro
Identification of a C-terminal Region That Regulates Mitogen-activated Protein Kinase Kinase-1 Cytoplasmic Localization and ERK Activation
J. Biol. Chem., December 14, 2001; 276(51): 48494 - 48501.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
G. Pearson, F. Robinson, T. Beers Gibson, B.-e Xu, M. Karandikar, K. Berman, and M. H. Cobb
Mitogen-Activated Protein (MAP) Kinase Pathways: Regulation and Physiological Functions
Endocr. Rev., April 1, 2001; 22(2): 153 - 183.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
J. F. Reilly, G. Mickey, and P. A. Maher
Association of Fibroblast Growth Factor Receptor 1 with the Adaptor Protein Grb14. CHARACTERIZATION OF A NEW RECEPTOR BINDING PARTNER
J. Biol. Chem., March 10, 2000; 275(11): 7771 - 7778.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Nantel, M. Huber, and D. Y. Thomas
Localization of Endogenous Grb10 to the Mitochondria and Its Interaction with the Mitochondrial-associated Raf-1 Pool
J. Biol. Chem., December 10, 1999; 274(50): 35719 - 35724.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Wang, H. Dai, N. Yousaf, M. Moussaif, Y. Deng, A. Boufelliga, O. R. Swamy, M. E. Leone, and H. Riedel
Grb10, a Positive, Stimulatory Signaling Adapter in Platelet-Derived Growth Factor BB-, Insulin-Like Growth Factor I-, and Insulin-Mediated Mitogenesis
Mol. Cell. Biol., September 1, 1999; 19(9): 6217 - 6228.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Morrione, P. Plant, B. Valentinis, O. Staub, S. Kumar, D. Rotin, and R. Baserga
mGrb10 Interacts with Nedd4
J. Biol. Chem., August 20, 1999; 274(34): 24094 - 24099.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. A. J. Janssen, K. G. Veenstra, P. Jonasch, E. Jonasch, and J. W. Mier
Ras- and Raf-induced Down-modulation of Non-muscle Tropomyosin Are MEK-independent
J. Biol. Chem., November 27, 1998; 273(48): 32182 - 32186.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Q. Dong, S. Porter, D. Hu, and F. Liu
Inhibition of hGrb10 Binding to the Insulin Receptor by Functional Domain-mediated Oligomerization
J. Biol. Chem., July 10, 1998; 273(28): 17720 - 17725.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. R. King, Y. Fang, E. S. Mahon, and D. H. Anderson
Using a Phage Display Library to Identify Basic Residues in A-Raf Required to Mediate Binding to the Src Homology 2 Domains of the p85 Subunit of Phosphatidylinositol 3'-Kinase
J. Biol. Chem., November 10, 2000; 275(46): 36450 - 36456.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Karandikar, S. Xu, and M. H. Cobb
MEKK1 Binds Raf-1 and the ERK2 Cascade Components
J. Biol. Chem., December 15, 2000; 275(51): 40120 - 40127.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. J. Lyons, R. Deane, D. K. Lynch, Z.-S. J. Ye, G. M. Sanderson, H. J. Eyre, G. R. Sutherland, and R. J. Daly
Identification of a Novel Human Tankyrase through Its Interaction with the Adaptor Protein Grb14
J. Biol. Chem., May 11, 2001; 276(20): 17172 - 17180.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Peruzzi, M. Prisco, A. Morrione, B. Valentinis, and R. Baserga
Anti-apoptotic Signaling of the Insulin-like Growth Factor-I Receptor through Mitochondrial Translocation of c-Raf and Nedd4
J. Biol. Chem., July 6, 2001; 276(28): 25990 - 25996.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1998 by the American Society for Biochemistry and Molecular Biology.