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J Biol Chem, Vol. 273, Issue 18, 11225-11233, May 1, 1998
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From the We investigated the molecular species of sulfated
sialyl Lewis X determinants, the putative L-selectin ligand, expressed
on high endothelial venules (HEV) in human lymph nodes. Comparison of
the reactivity pattern of HEV with the reactivity of the pure 6-sulfo,
6'-sulfo, or 6,6'-bissulfo sialyl Lewis X determinant with hitherto
known anti-sialyl Lewis X antibodies strongly suggested 6-sulfo sialyl
Lewis X to be the best candidate for the major sulfated sialyl Lewis X
determinant on HEV, followed by 6,6'-bissulfo sialyl Lewis X, whereas
6'-sulfo sialyl Lewis X was unlikely. We newly generated monoclonal
antibodies (mAbs) G152 and G72 directed against 6-sulfo sialyl Lewis X,
which intensely labeled HEV in immunohistochemical examination and
inhibited binding of recombinant L-selectin-IgG to HEV, suggesting that
the determinant serves as a ligand for L-selectin. To test the
concomitant expression of 6,6'-bissulfo sialyl Lewis X, specific mAbs
(G2706, G27011, G27037, and G27039) were generated, but all antibodies
failed to react to HEV. Next, we established mAbs (AG97 and AG273)
directed against 6-sulfo Lewis X, the asialo form of 6-sulfo sialyl
Lewis X. The antibodies were not reactive to untreated HEV, but
strongly reacted to sialidase-treated HEV. This indicated the
predominance of the sialylated form of 6-sulfo sialyl Lewis X and
minimal expression of its asialo form, corroborating that it was
synthesized by fucosyltransferase VII, the isoenzyme that
preferentially produces the sialylated form of the determinant.
Program of Experimental Pathology, Department of Applied Bio-organic Chemistry,
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
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S. Bhakta, A. Bartes, K. G. Bowman, W.-M. Kao, I. Polsky, J. K. Lee, B. N. Cook, R. E. Bruehl, S. D. Rosen, C. R. Bertozzi, et al. Sulfation of N-Acetylglucosamine by Chondroitin 6-Sulfotransferase 2 (GST-5) J. Biol. Chem., December 15, 2000; 275(51): 40226 - 40234. [Abstract] [Full Text] [PDF]
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F. M. El-Fasakhany, K. Uchimura, R. Kannagi, and T. Muramatsu A Novel Human Gal-3-O-Sulfotransferase. MOLECULAR CLONING, CHARACTERIZATION, AND ITS IMPLICATIONS IN BIOSYNTHESIS OF (SO4-3)Galbeta 1-4(Fucalpha 1-3)GlcNAc J. Biol. Chem., July 13, 2001; 276(29): 26988 - 26994. [Abstract] [Full Text] [PDF]
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