J Biol Chem, Vol. 273, Issue 18, 11302-11312, May 1, 1998
Membrane Association of Active Plasmid Partitioning Protein A
in Escherichia coli
Zhicheng
Lin and
Louis P.
Mallavia
From the Department of Microbiology, Washington State University,
Pullman, Washington 99164
QsopA and SopA, proteins essential for stable
maintenance of low copy number plasmids and encoded on plasmid QpH1 of
Coxiella burnetii and the F plasmid of Escherichia
coli, respectively, are shown to be membrane associated using
three independent approaches: isolation of hybrid protein A-PhoA
proteins that display PhoA (bacterial alkaline phosphatase) activity
indicating a periplasmic location, biochemical fractionation by
flotation gradient centrifugation, and subcellular localization by
immunoelectron microscopy. These data provide insight into the
mechanism by which partitioning protein A spatially directs plasmids
into daughter cells at bacterial division.
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.