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J Biol Chem, Vol. 273, Issue 18, 11302-11312, May 1, 1998

Membrane Association of Active Plasmid Partitioning Protein A in Escherichia coli

Zhicheng Lin and Louis P. Mallavia

From the Department of Microbiology, Washington State University, Pullman, Washington 99164

QsopA and SopA, proteins essential for stable maintenance of low copy number plasmids and encoded on plasmid QpH1 of Coxiella burnetii and the F plasmid of Escherichia coli, respectively, are shown to be membrane associated using three independent approaches: isolation of hybrid protein A-PhoA proteins that display PhoA (bacterial alkaline phosphatase) activity indicating a periplasmic location, biochemical fractionation by flotation gradient centrifugation, and subcellular localization by immunoelectron microscopy. These data provide insight into the mechanism by which partitioning protein A spatially directs plasmids into daughter cells at bacterial division.


Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
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