|
|
||||||||
J Biol Chem, Vol. 273, Issue 18, 11384-11391, May 1, 1998
From the Department of Biochemistry and Cell Biology, Institute of
Gerontology, Nippon Medical School, 1-396, Kosugi-cho, Nakahara-ku,
Kawasaki-city, Kanagawa 211-0063, Japan
An amount of human pro-apoptotic Bax as low as
0.01% of total protein was sufficient to cause cell death in
Escherichia coli. The bacterial cell death was examined
using a viable bacteria-specific fluorescence indicator system and loss
of colony formation ability. Co-expression of anti-apoptotic
Bcl-xL showed a modest inhibitory effect on the cell death
caused by Bax. The trace amount of Bax elongated E. coli
and accumulated monounsaturated fatty acids, suggesting an
unusual metabolism of redox in the host. In fact, an increase of
KCN-dependent O2 consumption accompanied the
expression of Bax. At the same time, a fluorescent pH indicator showed
the apparent accumulation of protons outside the cell, suggesting that
the membrane is intact. Bax increased the level of superoxide anion as
measured by the expression of superoxide-dependent
promoter. Nicked DNA was significantly generated, and the frequency of
mutations resistant to rifampicin was increased by 30-fold, depending
upon the expression of Bax. It is proposed that trace amounts of Bax increase oxygen consumption, triggering generation of superoxide, which
affects DNA, leading to bacterial death.
A Trace Amount of the Human Pro-apoptotic Factor Bax Induces
Bacterial Death Accompanied by Damage of DNA
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
![]()
CiteULike
Complore
Connotea
Del.icio.us
Digg
Reddit
Technorati What's this?
This article has been cited by other articles:
![]() |
D. J. Adams, L. van der Weyden, A. Mayeda, S. Stamm, B. J. Morris, and J. E.J. Rasko ZNF265--a novel spliceosomal protein able to induce alternative splicing J. Cell Biol., July 9, 2001; 154(1): 25 - 32. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. C. Kampranis, R. Damianova, M. Atallah, G. Toby, G. Kondi, P. N. Tsichlis, and A. M. Makris A Novel Plant Glutathione S-Transferase/Peroxidase Suppresses Bax Lethality in Yeast J. Biol. Chem., September 15, 2000; 275(38): 29207 - 29216. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |