| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 19, 11514-11520, May 8, 1998
§ and§¶
From the A major mechanism by which protein kinase C (PKC)
function is regulated is through the selective targeting and activation of individual PKC isotypes at distinct subcellular locations. PKC
Sealy Center for Oncology and Hematology and
the Departments of § Human Biological Chemistry and Genetics
and ¶ Pharmacology, University of Texas Medical Branch,
Galveston, Texas 77555-1048
II is selectively activated at the nucleus during
G2 phase of cell cycle where it is required for entry into
mitosis. Selective nuclear activation of PKC II is
conferred by molecular determinants within the carboxyl-terminal
catalytic domain of the kinase (Walker, S. D., Murray, N. R.,
Burns, D. J., and Fields, A. P. (1995) Proc. Natl.
Acad. Sci. U. S. A. 92, 9156-9160). We previously described a
lipid-like PKC activator in nuclear membranes, termed nuclear membrane
activation factor (NMAF), that potently stimulates PKC II activity through interactions involving this domain
(Murray, N. R., Burns, D. J., and Fields, A. P. (1994)
J. Biol. Chem. 269, 21385-21390). We have now
identified NMAF as phosphatidylglycerol (PG), based on several lines of
evidence. First, NMAF cofractionates with PG as a single peak of
activity through multiple chromatographic separations and exhibits
phospholipase sensitivity identical to that of PG. Second, purified PG,
but not other phospholipids, exhibits dose-dependent NMAF
activity. Third, defined molecular species of PG exhibit different
abilities to stimulate PKC II activity. 1,2-Dioleoyl-PG
possesses significantly higher activity than other PG species,
suggesting that both fatty acid side chain composition and the glycerol
head group are important determinants for activity. Fourth, in
vitro binding studies demonstrate that PG binds to the
carboxyl-terminal region of PKC II, the same region we
previously implicated in NMAF-mediated activation of PKC
II. Taken together, our results indicate that specific
molecular species of nuclear PG function to physiologically regulate
PKC II activity at the nucleus.
This article has been cited by other articles:
|
|
 |
|
  Q. Zhong, G. Li, J. Gvozdenovic-Jeremic, and M. L. Greenberg Up-regulation of the Cell Integrity Pathway in Saccharomyces cerevisiae Suppresses Temperature Sensitivity of the pgs1{Delta} Mutant J. Biol. Chem., June 1, 2007; 282(22): 15946 - 15953. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yang, J. Cao, and Y. Shi Identification and Characterization of a Gene Encoding Human LPGAT1, an Endoplasmic Reticulum-associated Lysophosphatidylglycerol Acyltransferase J. Biol. Chem., December 31, 2004; 279(53): 55866 - 55874. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Schultz, M. Ling, and C. Larsson Identification of an Amino Acid Residue in the Protein Kinase C C1b Domain Crucial for Its Localization to the Golgi Network J. Biol. Chem., July 23, 2004; 279(30): 31750 - 31760. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. F. Irvine Nuclear Lipid Signaling Sci. Signal., September 17, 2002; 2002(150): re13 - re13. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Chiarini, J. F. Whitfield, U. Armato, and I. Dal Pra Protein Kinase C-beta II Is an Apoptotic Lamin Kinase in Polyomavirus-transformed, Etoposide-treated pyF111 Rat Fibroblasts J. Biol. Chem., May 17, 2002; 277(21): 18827 - 18839. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Deacon, T. R. Pettitt, P. Webb, T. Cross, H. Chahal, M. J. O. Wakelam, and J. M. Lord Generation of diacylglycerol molecular species through the cell cycle: a role for 1-stearoyl, 2-arachidonyl glycerol in the activation of nuclear protein kinase C-{beta}II at G2/M J. Cell Sci., January 3, 2002; 115(5): 983 - 989. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hiroyama and T. Takenawa Isolation of a cDNA Encoding Human Lysophosphatidic Acid Phosphatase That Is Involved in the Regulation of Mitochondrial Lipid Biosynthesis J. Biol. Chem., October 8, 1999; 274(41): 29172 - 29180. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. RON and M. G. KAZANIETZ New insights into the regulation of protein kinase C and novel phorbol ester receptors FASEB J, October 1, 1999; 13(13): 1658 - 1676. [Abstract] [Full Text]
|
|
|
|
|
|
K. Kawasaki, O. Kuge, S.-C. Chang, P. N. Heacock, M. Rho, K. Suzuki, M. Nishijima, and W. Dowhan Isolation of a Chinese Hamster Ovary (CHO) cDNA Encoding Phosphatidylglycerophosphate (PGP) Synthase, Expression of Which Corrects the Mitochondrial Abnormalities of a PGP Synthase-defective Mutant of CHO-K1 Cells J. Biol. Chem., January 15, 1999; 274(3): 1828 - 1834. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P Collas Sequential PKC- and Cdc2-mediated phosphorylation events elicit zebrafish nuclear envelope disassembly J. Cell Sci., January 3, 1999; 112(6): 977 - 987. [Abstract] [PDF]
|
|
|
|
|
|
L. M. Neri, P. Borgatti, S. Capitani, and A. M. Martelli Nuclear Diacylglycerol Produced by Phosphoinositide-specific Phospholipase C Is Responsible for Nuclear Translocation of Protein Kinase C-alpha J. Biol. Chem., November 6, 1998; 273(45): 29738 - 29744. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Gokmen-Polar and A. P. Fields Mapping of a Molecular Determinant for Protein Kinase C beta II Isozyme Function J. Biol. Chem., August 7, 1998; 273(32): 20261 - 20266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. G. Stebbins and D. Mochly-Rosen Binding Specificity for RACK1 Resides in the V5 Region of beta II Protein Kinase C J. Biol. Chem., August 3, 2001; 276(32): 29644 - 29650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. T. O'Flaherty, B. A. Chadwell, M. W. Kearns, S. Sergeant, and L. W. Daniel Protein Kinases C Translocation Responses to Low Concentrations of Arachidonic Acid J. Biol. Chem., June 29, 2001; 276(27): 24743 - 24750. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
