| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 19, 11556-11562, May 8, 1998
1,3-D-Mannoside
1,4-N-Acetylglucosaminyltransferase IV
From the Central Laboratories for Key Technology, KIRIN Brewery
Co., Ltd., 1-13-5 Fuku-ura, Kanazawa-ku,
Yokohama, 236-0004, Japan
UDP-N-acetylglucosamine:
1,3-D-mannoside
1,4-N-acetylglucosaminyltransferase (GnT-IV) is one of the essential
enzymes in the production of tri- and tetra-antennary Asn-linked sugar
chains. Recently, we have successfully purified GnT-IV from bovine
small intestine. Based on the partial amino acid sequence of the
purified bovine GnT-IV enzyme, its cDNA has been cloned from bovine
small intestine. The open reading frame is 1,605 base pairs long, and this sequence produced GnT-IV activity on transient expression in COS-7
cells. Although the deduced amino acid sequence does not have any
significant homology with other known
N-acetylglucosaminyltransferases (GnTs), the hydrophobicity
profile showed a typical type II transmembrane protein structure, which
is common to many glycosyltransferases. N-terminal amino acid
sequencing of the purified GnT-IV revealed that 92 amino acids,
including a transmembrane region, were truncated during purification.
Of the three potential N-glycosylation sites Asn-458 was
actually glycosylated in the purified enzyme, although this
N-glycosylation site could be abolished without any
reduction in GnT-IV activity. Serial deletions at both the N and C
termini proved that the catalytic domain of GnT-IV is located in the
central region of the enzyme. The GnT-IV mRNA level correlated with
enzymatic activity in the various bovine tissues tested.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Uemura, T. Kurose, T. Suzuki, S. Yoshida, M. Ito, M. Saito, M. Horiuchi, F. Inagaki, Y. Igarashi, and J.-i. Inokuchi Substitution of the N-glycan function in glycosyltransferases by specific amino acids: ST3Gal-V as a model enzyme Glycobiology, March 1, 2006; 16(3): 258 - 270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kobata The history of glycobiology in Japan Glycobiology, August 1, 2001; 11(8): 99R - 105R. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. L. Christensen, U. B. Jensen, P. Bross, and T. F. Orntoft The C-terminal N-glycosylation sites of the human {alpha}1,3/4-fucosyltransferase III, -V, and -VI (hFucTIII, -V and -VI) are necessary for the expression of full enzyme activity Glycobiology, September 1, 2000; 10(9): 931 - 939. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Fukuta, R. Abe, T. Yokomatsu, N. Kono, M. Asanagi, F. Omae, M. T. Minowa, M. Takeuchi, and T. Makino Remodeling of sugar chain structures of human interferon-{gamma} Glycobiology, April 1, 2000; 10(4): 421 - 430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Nakayama, J.-C. Yeh, A. K. Misra, S. Ito, T. Katsuyama, and M. Fukuda Expression cloning of a human alpha 1,4-N-acetylglucosaminyltransferase that forms GlcNAcalpha 1right-arrow4Galbeta right-arrowR, a glycan specifically expressed in the gastric gland mucous cell-type mucin PNAS, August 3, 1999; 96(16): 8991 - 8996. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Takamatsu, S. Oguri, M. T. Minowa, A. Yoshida, K. Nakamura, M. Takeuchi, and A. Kobata Unusually High Expression of N-Acetylglucosaminyltransferase-IVa in Human Choriocarcinoma Cell Lines: A Possible Enzymatic Basis of the Formation of Abnormal Biantennary Sugar Chain Cancer Res., August 1, 1999; 59(16): 3949 - 3953. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Taguchi, T. Ogawa, S. Inoue, Y. Inoue, Y. Sakamoto, H. Korekane, and N. Taniguchi Purification and Characterization of UDP-GlcNAc: GlcNAcbeta 1-6(GlcNAcbeta 1-2)Manalpha 1-R [GlcNAc to Man]-beta 1, 4-N-acetylglucosaminyltransferase VI from Hen Oviduct J. Biol. Chem., October 13, 2000; 275(42): 32598 - 32602. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Sakamoto, T. Taguchi, K. Honke, H. Korekane, H. Watanabe, Y. Tano, N. Dohmae, K. Takio, A. Horii, and N. Taniguchi Molecular Cloning and Expression of cDNA Encoding Chicken UDP-N-acetyl-D-glucosamine (GlcNAc): GlcNAcbeta 1-6(GlcNAcbeta 1-2)- Manalpha 1-R[GlcNAc to Man]beta 1,4N-acetylglucosaminyltransferase VI J. Biol. Chem., November 10, 2000; 275(46): 36029 - 36034. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
