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J Biol Chem, Vol. 273, Issue 19, 11605-11610, May 8, 1998

Sequences Outside Recognition Sets Are Not Neutral for tRNA Aminoacylation
EVIDENCE FOR NONPERMISSIVE COMBINATIONS OF NUCLEOTIDES IN THE ACCEPTOR STEM OF YEAST tRNA^Phe

From Unité Propre de Recherche n° 9002 du CNRS, "Structure des Macromolécules Biologiques et Mécanismes de Reconnaissance", Institut de Biologie Moléculaire et Cellulaire, 15, rue René Descartes, 67084 Strasbourg Cedex, France

Phenylalanine identity of yeast tRNA^Phe is governed by five nucleotides including residues A73, G20, and the three anticodon nucleotides (Sampson et al., 1989, Science 243, 1363-1366). Analysis of in vitro transcripts derived from yeast tRNA^Phe and Escherichia coli tRNA^Ala bearing these recognition elements shows that phenylalanyl-tRNA synthetase is sensitive to additional nucleotides within the acceptor stem. Insertion of G2-C71 has dramatic negative effects in both tRNA frameworks. These effects become compensated by a second-site mutation, the insertion of the wobble G3-U70 pair, which by itself has no effect on phenylalanylation. From a mechanistic point of view, the G2-C71/G3-U70 combination is not a "classical" recognition element since its antideterminant effect is compensated for by a second-site mutation.

This enlarges our understanding of tRNA identity that appears not only to be the outcome of a combination of positive and negative signals forming the so-called recognition/identity set but that is also based on the presence of nonrandom combinations of sequences elsewhere in tRNA. These sequences, we name "permissive elements," are retained by evolution so that they do not hinder aminoacylation. Likely, no nucleotide within a tRNA is of random nature but has been selected so that a tRNA can fulfill all its functions efficiently.

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