JBC Avanti Polar Lipids

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Howe, L.
Right arrow Articles by Ausió, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Howe, L.
Right arrow Articles by Ausió, J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 273, Issue 19, 11625-11629, May 8, 1998

Folding of Chromatin in the Presence of Heterogeneous Histone H1 Binding to Nucleosomes

LeAnn Howe, Maya Iskandar, and Juan Ausió

From the Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia V8W 3P6, Canada

We have reconstituted oligonucleosome complexes containing histone H1 starting from a synthetic DNA template, consisting of 12 tandemly arranged 208-base pair fragments of the 5 S rRNA gene, purified HeLa histone octamers, and histone H1. A ratio of histone H1 per histone octamer used in the reconstitution (0.8-0.9 mol of histone H1/mol of histone octamer) similar to that observed in vivo was used. The reconstituted chromatin complexes exhibit a salt-dependent folding, which is almost indistinguishable from that exhibited by chromatin fragments obtained from nuclease digestion of native chromatin. The folding of this reconstituted chromatin complex seems to be rather independent of the symmetrical or asymmetrical position occupied by H1 in the individual nucleosomes. Binding of histone H1 to the oligonucleosome complexes, under the stoichiometric binding conditions used, had no inhibitory effect on the transcriptional potential of these complexes.

Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Biophys. JHome page
N. Kepper, D. Foethke, R. Stehr, G. Wedemann, and K. Rippe
Nucleosome Geometry and Internucleosomal Interactions Control the Chromatin Fiber Conformation
Biophys. J., October 15, 2008; 95(8): 3692 - 3705.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. Ishibashi, K. So, C. G. Cupples, and J. Ausio
MBD4-Mediated Glycosylase Activity on a Chromatin Template Is Enhanced by Acetylation
Mol. Cell. Biol., August 1, 2008; 28(15): 4734 - 4744.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
R. M. Finn, K. Browne, K. C. Hodgson, and J. Ausio
sNASP, a Histone H1-Specific Eukaryotic Chaperone Dimer that Facilitates Chromatin Assembly
Biophys. J., August 1, 2008; 95(3): 1314 - 1325.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. A. Hill, C. L. Peterson, and A. N. Imbalzano
Effects of HMGN1 on Chromatin Structure and SWI/SNF-mediated Chromatin Remodeling
J. Biol. Chem., December 16, 2005; 280(50): 41777 - 41783.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. J. M. Jason, R. M. Finn, G. Lindsey, and J. Ausio
Histone H2A Ubiquitination Does Not Preclude Histone H1 Binding, but It Facilitates Its Association with the Nucleosome
J. Biol. Chem., February 11, 2005; 280(6): 4975 - 4982.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Contreras, T. K. Hale, D. L. Stenoien, J. M. Rosen, M. A. Mancini, and R. E. Herrera
The Dynamic Mobility of Histone H1 Is Regulated by Cyclin/CDK Phosphorylation
Mol. Cell. Biol., December 1, 2003; 23(23): 8626 - 8636.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. C. Bash, J. Yodh, Y. Lyubchenko, N. Woodbury, and D. Lohr
Population Analysis of Subsaturated 172-12 Nucleosomal Arrays by Atomic Force Microscopy Detects Nonrandom Behavior That Is Favored by Histone Acetylation and Short Repeat Length
J. Biol. Chem., December 14, 2001; 276(51): 48362 - 48370.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Rabbani, M. Iskandar, and J. Ausio
Daunomycin-induced Unfolding and Aggregation of Chromatin
J. Biol. Chem., June 25, 1999; 274(26): 18401 - 18406.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. M. Carruthers and J. C. Hansen
The Core Histone N Termini Function Independently of Linker Histones during Chromatin Condensation
J. Biol. Chem., November 17, 2000; 275(47): 37285 - 37290.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Hellauer, E. Sirard, and B. Turcotte
Decreased Expression of Specific Genes in Yeast Cells Lacking Histone H1
J. Biol. Chem., April 20, 2001; 276(17): 13587 - 13592.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1998 by the American Society for Biochemistry and Molecular Biology.