| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Vol. 273, Issue 2, 749-755, January 9, 1998
From the Department of Life Science, National Tsing Hua University,
Hsinchu, Taiwan 30043, Republic of China
We have previously shown that treatment with
okadaic acid (OA) followed by heat shock (HS) (termed OA 
HS
treatment) leads to rapid transactivation of the 78-kDa
glucose-regulated protein gene (grp78) in 9L rat brain
tumor cells. A cAMP-responsive element-like (CRE-like, TGACGTGA)
promoter sequence and a protein kinase A signaling pathway are involved
in this induction, and activation of both CRE binding protein (CREB)
and activating transcription factor-2 (ATF-2) is required in the above
process. Herein, we report that transactivation of grp78,
as well as phosphorylation/activation of ATF-2, can be completely
annihilated by SB203580, a highly specific inhibitor of p38
mitogen-activated protein kinase (p38MAPK). Activation of
p38MAPK by OA HS is also substantiated by its own
phosphorylation as well as the phosphorylation and activation of MAPK
activating protein kinase-2 in cells subjected to this treatment. The
involvement of p38MAPK in the activation of ATF-2, which
leads to the transactivation of rat grp78, is confirmed by
electrophoretic mobility shift assay using a probe containing the
CRE-like sequence as well as by transient transfection assays with a
plasmid containing a 710-base pair stretch of the grp78
promoter. Together with our previous studies, these results led us to
conclude that phosphorylation/activation of CREB upon OA HS
treatment is mediated by cAMP-dependent protein kinase,
whereas that of ATF-2 is mediated by p38MAPK. The
transcription factors may bind to each other to form heterodimers that
in turn transactivate grp78 by binding to the CRE-like
element. This suggests that distinct signaling pathways converge on
CREB-ATF-2, where each subunit is individually activated by a specific
class of protein kinases. This may allow modulation of
grp78 transactivation by diverse external stimuli.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J.-C. Chen, M.-L. Wu, K.-C. Huang, and W.-W. Lin HMG-CoA reductase inhibitors activate the unfolded protein response and induce cytoprotective GRP78 expression Cardiovasc Res, July 2, 2008; (2008) cvn160v2. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Birrell, S. Wong, K. McCluskie, M. C. Catley, E. L. Hardaker, S. Haj-Yahia, and M. G. Belvisi Second-Generation Inhibitors Demonstrate the Involvement of p38 Mitogen-Activated Protein Kinase in Post-Transcriptional Modulation of Inflammatory Mediator Production in Human and Rodent Airways J. Pharmacol. Exp. Ther., March 1, 2006; 316(3): 1318 - 1327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. S. Gardner, C.-W. Shiau, C.-S. Chen, and L. M. Graves Peroxisome Proliferator-activated Receptor {gamma}-independent Activation of p38 MAPK by Thiazolidinediones Involves Calcium/Calmodulin-dependent Protein Kinase II and Protein Kinase R: CORRELATION WITH ENDOPLASMIC RETICULUM STRESS J. Biol. Chem., March 18, 2005; 280(11): 10109 - 10118. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Campbell, C. J. Ciesielski, A. E. Hunt, N. J. Horwood, J. T. Beech, L. A. Hayes, A. Denys, M. Feldmann, F. M. Brennan, and B. M. J. Foxwell A Novel Mechanism for TNF-{alpha} Regulation by p38 MAPK: Involvement of NF-{kappa}B with Implications for Therapy in Rheumatoid Arthritis J. Immunol., December 1, 2004; 173(11): 6928 - 6937. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-I Chang, B. Zoghi, J. C. Liao, and L. Kuo The Involvement of Tyrosine Kinases, Cyclic AMP/Protein Kinase A, and p38 Mitogen-Activated Protein Kinase in IL-13-Mediated Arginase I Induction in Macrophages: Its Implications in IL-13-Inhibited Nitric Oxide Production J. Immunol., August 15, 2000; 165(4): 2134 - 2141. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Engelman, A. H. Berg, R. Y. Lewis, A. Lin, M. P. Lisanti, and P. E. Scherer Constitutively Active Mitogen-activated Protein Kinase Kinase 6 (MKK6) or Salicylate Induces Spontaneous 3T3-L1 Adipogenesis J. Biol. Chem., December 10, 1999; 274(50): 35630 - 35638. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Rolli, A. Kotlyarov, K. M. Sakamoto, M. Gaestel, and A. Neininger Stress-induced Stimulation of Early Growth Response Gene-1 by p38/Stress-activated Protein Kinase 2 Is Mediated by a cAMP-responsive Promoter Element in a MAPKAP Kinase 2-independent Manner J. Biol. Chem., July 9, 1999; 274(28): 19559 - 19564. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-J. Hung, T.-J. Cheng, Y.-K. Lai, and M. D.-T. Chang Differential Activation of p38 Mitogen-activated Protein Kinase and Extracellular Signal-regulated Protein Kinases Confers Cadmium-induced HSP70 Expression in 9L Rat Brain Tumor Cells J. Biol. Chem., November 27, 1998; 273(48): 31924 - 31931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Engelman, M. P. Lisanti, and P. E. Scherer Specific Inhibitors of p38 Mitogen-activated Protein Kinase Block 3T3-L1 Adipogenesis J. Biol. Chem., November 27, 1998; 273(48): 32111 - 32120. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Tirasophon, A. A. Welihinda, and R. J. Kaufman A stress response pathway from the endoplasmic reticulum to the nucleus requires a novel bifunctional protein kinase/endoribonuclease (Ire1p) in mammalian cells Genes & Dev., June 15, 1998; 12(12): 1812 - 1824. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
