HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Richards, O. C. Articles by Ehrenfeld, E. Search for Related Content PubMed PubMed Citation Articles by Richards, O. C. Articles by Ehrenfeld, E. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 21, 12832-12840, May 22, 1998

Effects of Poliovirus 3AB Protein on 3D Polymerase-catalyzed Reaction

From the Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697

Poliovirus RNA replication requires the activities of a viral RNA-dependent RNA polymerase, 3D^pol, in conjunction with several additional viral and likely cellular proteins. The importance of both the 3A and 3B coding regions has been documented previously by genetic tests, and their biochemical activities have been the subject of several recent investigations. In this study, we examined the previously reported stimulation of 3D-catalyzed RNA synthesis by 3AB. We show that 3AB does not stimulate RNA synthesis on templates that are stably base paired to a primer, indicating that 3AB does not stabilize or otherwise activate 3D^pol for chain elongation. Similarly, it does not alter the kinetic parameters or binding affinities of 3D for substrates. In the absence of a primer, or in the presence of a primer that does not form a stable hybrid with the template, 3AB increases the utilization of 3'-hydroxyl termini as sites for chain elongation by 3D, and thereby stimulates RNA synthesis. 3AB may interact with and stabilize these sites and/or may recruit 3D^pol to the site, resulting in stimulation of the initiation of elongation events. We propose that this activity is required for stabilizing weak interactions that occur during nucleotidyl-protein-primed initiation events in the viral RNA replication complex.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				A. M. De Palma, H. J. Thibaut, L. van der Linden, K. Lanke, W. Heggermont, S. Ireland, R. Andrews, M. Arimilli, T. H. Al-Tel, E. De Clercq, et al. Mutations in the Nonstructural Protein 3A Confer Resistance to the Novel Enterovirus Replication Inhibitor TTP-8307 Antimicrob. Agents Chemother., May 1, 2009; 53(5): 1850 - 1857. [Abstract] [Full Text] [PDF]

				J. Yin, Y. Liu, E. Wimmer, and A. V. Paul Complete protein linkage map between the P2 and P3 non-structural proteins of poliovirus J. Gen. Virol., August 1, 2007; 88(8): 2259 - 2267. [Abstract] [Full Text] [PDF]

				D. M. Strauss and D. S. Wuttke Characterization of Protein-Protein Interactions Critical for Poliovirus Replication: Analysis of 3AB and VPg Binding to the RNA-Dependent RNA Polymerase J. Virol., June 15, 2007; 81(12): 6369 - 6378. [Abstract] [Full Text] [PDF]

				J. J. DeStefano and O. Titilope Poliovirus Protein 3AB Displays Nucleic Acid Chaperone and Helix-Destabilizing Activities J. Virol., February 15, 2006; 80(4): 1662 - 1671. [Abstract] [Full Text] [PDF]

				A. B. Chetverin, D. S. Kopein, H. V. Chetverina, A. A. Demidenko, and V. I. Ugarov Viral RNA-directed RNA Polymerases Use Diverse Mechanisms to Promote Recombination between RNA Molecules J. Biol. Chem., March 11, 2005; 280(10): 8748 - 8755. [Abstract] [Full Text] [PDF]

				S. S. Choe and K. Kirkegaard Intracellular Topology and Epitope Shielding of Poliovirus 3A Protein J. Virol., June 1, 2004; 78(11): 5973 - 5982. [Abstract] [Full Text] [PDF]

				C. T. Cornell, R. Perera, J. E. Brunner, and B. L. Semler Strand-Specific RNA Synthesis Determinants in the RNA-Dependent RNA Polymerase of Poliovirus J. Virol., May 1, 2004; 78(9): 4397 - 4407. [Abstract] [Full Text] [PDF]

				N. L. Teterina, M. S. Rinaudo, and E. Ehrenfeld Strand-Specific RNA Synthesis Defects in a Poliovirus with a Mutation in Protein 3A J. Virol., December 1, 2003; 77(23): 12679 - 12691. [Abstract] [Full Text] [PDF]

				M. H. Fogg, N. L. Teterina, and E. Ehrenfeld Membrane Requirements for Uridylylation of the Poliovirus VPg Protein and Viral RNA Synthesis In Vitro J. Virol., November 1, 2003; 77(21): 11408 - 11416. [Abstract] [Full Text] [PDF]

				C. L. Fata, S. G. Sawicki, and D. L. Sawicki Modification of Asn374 of nsP1 Suppresses a Sindbis Virus nsP4 Minus-Strand Polymerase Mutant J. Virol., July 29, 2002; 76(17): 8641 - 8649. [Abstract] [Full Text] [PDF]

				J. M. Lyle, A. Clewell, K. Richmond, O. C. Richards, D. A. Hope, S. C. Schultz, and K. Kirkegaard Similar Structural Basis for Membrane Localization and Protein Priming by an RNA-dependent RNA Polymerase J. Biol. Chem., May 3, 2002; 277(18): 16324 - 16331. [Abstract] [Full Text] [PDF]

				D. A. Dodd, T. H. Giddings Jr., and K. Kirkegaard Poliovirus 3A Protein Limits Interleukin-6 (IL-6), IL-8, and Beta Interferon Secretion during Viral Infection J. Virol., September 1, 2001; 75(17): 8158 - 8165. [Abstract] [Full Text] [PDF]

				V. Rodriguez-Wells, S. J. Plotch, and J. J. DeStefano Primer-dependent synthesis by poliovirus RNA-dependent RNA polymerase (3Dpol) Nucleic Acids Res., July 1, 2001; 29(13): 2715 - 2724. [Abstract] [Full Text] [PDF]

				E. Sadowy, A. Maasen, M. Juszczuk, C. David, W. Zagórski-Ostoja, B. Gronenborn, and M. D. Hulanicka The ORF0 product of Potato leafroll virus is indispensable for virus accumulation J. Gen. Virol., June 1, 2001; 82(6): 1529 - 1532. [Abstract] [Full Text]

				L. Tomei, R. L. Vitale, I. Incitti, S. Serafini, S. Altamura, A. Vitelli, and R. De Francesco Biochemical characterization of a hepatitis C virus RNA-dependent RNA polymerase mutant lacking the C-terminal hydrophobic sequence J. Gen. Virol., March 1, 2000; 81(3): 759 - 767. [Abstract] [Full Text]

				J. J. Arnold, S. K. B. Ghosh, and C. E. Cameron Poliovirus RNA-dependent RNA Polymerase (3Dpol). DIVALENT CATION MODULATION OF PRIMER, TEMPLATE, AND NUCLEOTIDE SELECTION J. Biol. Chem., December 24, 1999; 274(52): 37060 - 37069. [Abstract] [Full Text] [PDF]

				Y. C. Bell, B. L. Semler, and E. Ehrenfeld Requirements for RNA Replication of a Poliovirus Replicon by Coxsackievirus B3 RNA Polymerase J. Virol., November 1, 1999; 73(11): 9413 - 9421. [Abstract] [Full Text] [PDF]

				J. J. Arnold and C. E. Cameron Poliovirus RNA-dependent RNA Polymerase (3Dpol) Is Sufficient for Template Switching in Vitro J. Biol. Chem., January 29, 1999; 274(5): 2706 - 2716. [Abstract] [Full Text] [PDF]