| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 21, 12999-13006, May 22, 1998
From the Department of Biostructure and Function, School of Dental
Medicine, University of Connecticut Health Center, Farmington,
Connecticut 06030-3705
Each globular domain of exported multiglobular
proteins putatively undergoes chaperone surveillance in the endoplasmic
reticulum lumen. It is difficult to visualize how surveillance of
multiple globular domains might be orchestrated and regulated. Aggrecan core protein has been used as a prototype for this problem by examining
transfection of informative constructs into Chinese hamster ovary
cells. The salient results are as follows: 1) aggrecan's N-terminal G1
domain is minimally secreted, and its flanking Golgi reporter sites are
not decorated with glycsoaminoglycan chains; in contrast, its
C-terminal G3 domain is readily secreted with flanking GAG chains, and
G3 also facilitates G1 secretion; 2) G3 but not G1 can be
intracellularly cross-linked to chaperone Hsp25; 3) G3 and Hsp25 remain
noncovalently bound and are secreted together when G3 is situated
N-terminal to its normal location; 4) exon 15, which encodes the center
of G3's C-lectin subdomain, is necessary and sufficient for G3
secretion. A model is proposed in which Hsp25 piggybacks onto nascent
G3 in the cytosol during a translocational pause and enters the ER
lumen with G3, and once G3 properly folds, Hsp25 releases G3 and
recycles to the nucleus while G3 continues to the Golgi stacks,
providing passage for the entire core protein.
This article has been cited by other articles:
|
|
 |
|
  J. M. Day, A. I. Olin, A. D. Murdoch, A. Canfield, T. Sasaki, R. Timpl, T. E. Hardingham, and A. Aspberg Alternative Splicing in the Aggrecan G3 Domain Influences Binding Interactions with Tenascin-C and Other Extracellular Matrix Proteins J. Biol. Chem., March 26, 2004; 279(13): 12511 - 12518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Basha, G. J. Lee, L. A. Breci, A. C. Hausrath, N. R. Buan, K. C. Giese, and E. Vierling The Identity of Proteins Associated with a Small Heat Shock Protein during Heat Stress in Vivo Indicates That These Chaperones Protect a Wide Range of Cellular Functions J. Biol. Chem., February 27, 2004; 279(9): 7566 - 7575. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Lee, L. Chen, F. Paiwand, L. Cao, Y. Wu, R. Inman, M. E. Adams, and B. B. Yang Cleavage of the Carboxyl Tail from the G3 Domain of Aggrecan but Not Versican and Identification of the Amino Acids Involved in the Degradation J. Biol. Chem., June 14, 2002; 277(25): 22279 - 22288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Day, A. D. Murdoch, and T. E. Hardingham The Folded Protein Modules of the C-terminal G3 Domain of Aggrecan Can Each Facilitate the Translocation and Secretion of the Extended Chondroitin Sulfate Attachment Sequence J. Biol. Chem., December 31, 1999; 274(53): 38107 - 38111. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. L. Yang, L. Cao, C. Kiani, V. Lee, Y. Zhang, M. E. Adams, and B. B. Yang Tandem Repeats Are Involved in G1 Domain Inhibition of Versican Expression and Secretion and the G3 Domain Enhances Glycosaminoglycan Modification and Product Secretion via the Complement-binding Protein-like Motif J. Biol. Chem., July 7, 2000; 275(28): 21255 - 21261. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Domowicz, E. W. Pirok III, T. E. Novak, and N. B. Schwartz Role of the C-terminal G3 Domain in Sorting and Secretion of Aggrecan Core Protein and Ubiquitin-mediated Degradation of Accumulated Mutant Precursors J. Biol. Chem., November 3, 2000; 275(45): 35098 - 35105. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Chen, Y. Wu, V. Lee, C. Kiani, M. E. Adams, Y. Yao, and B. B. Yang The Folded Modules of Aggrecan G3 Domain Exert Two Separable Functions in Glycosaminoglycan Modification and Product Secretion J. Biol. Chem., January 18, 2002; 277(4): 2657 - 2665. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
