| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 21, 13119-13128, May 22, 1998
,,,,
,, and
From the Although the available evidence suggests
that whereas the caspase family plays a major role in apoptosis, they
are not the sole stimulators of death. A random yeast two-hybrid screen
of a lymphocyte cDNA library (using caspase-3 as the bait) found an
interaction between caspase-3 and the regulatory subunit A In Jurkat cells induced to undergo apoptosis with anti-Fas antibody,
protein phosphatase 2A (PP2A) activity increased 4.5-fold after 6 h. By 12 h, the regulatory A Department of Biochemistry and
§ Department of Cell Biology, Parke-Davis Pharmaceutical
Research Division, Warner-Lambert Company, Ann Arbor, Michigan 48105, the ¶ Department of Molecular Biology, the
Department of Biochemistry, and the
Department of Immunology, BASF Bioresearch Corporation,
Worcester, Massachusetts 01605, and the ** Department of Pathology,
University of California at San Diego,
La Jolla, California 92093
of
protein phosphatase 2A. This protein was found to be a substrate for
caspase-3, but not caspase-1, and could compete effectively against
either a protein or synthetic peptide substrate.
subunit could no longer be
detected in cell lysates. There was no change in the amount of the
catalytic subunit. The effects on PP2A could be prevented by the
caspase family inhibitors acetyl-Asp-Glu-Val-Asp (DEVD) aldehyde or Ac-DEVD fluoromethyl ketone. The mitogen-activated protein (MAP) kinase pathway is regulated by PP2A. At 12 h after the addition of anti-Fas antibody, a decrease in the amount of the
phosphorylated forms of MAP kinase was observed. Again, this loss of
activated MAP kinase could be prevented by the addition of DEVD-cho or
DEVD-fmk. These data are consistent with a pathway whereby induction of
apoptosis activates caspase-3. This enzyme then cleaves the regulatory
A subunit of PP2A, increasing its activity. These data show that the
activated PP2A will then effect a change in the phosphorylation state
of the cell. These data provide a link between the caspases and signal
transduction pathways.
This article has been cited by other articles:
|
|
 |
|
  M. M. Lurtz and C. F. Louis Purinergic Receptor Mediated Regulation of Lens Connexin43 Invest. Ophthalmol. Vis. Sci., September 1, 2007; 48(9): 4177 - 4186. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Sun, G. Stoecklin, S. Van Way, V. Hinkovska-Galcheva, R.-F. Guo, P. Anderson, and T. P. Shanley Tristetraprolin (TTP)-14-3-3 Complex Formation Protects TTP from Dephosphorylation by Protein Phosphatase 2a and Stabilizes Tumor Necrosis Factor-{alpha} mRNA J. Biol. Chem., February 9, 2007; 282(6): 3766 - 3777. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Dessauge, X. Cayla, J. P. Albar, A. Fleischer, A. Ghadiri, M. Duhamel, and A. Rebollo Identification of PP1{alpha} as a Caspase-9 Regulator in IL-2 Deprivation-Induced Apoptosis J. Immunol., August 15, 2006; 177(4): 2441 - 2451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Alvarado-Kristensson and T. Andersson Protein Phosphatase 2A Regulates Apoptosis in Neutrophils by Dephosphorylating Both p38 MAPK and Its Substrate Caspase 3 J. Biol. Chem., February 18, 2005; 280(7): 6238 - 6244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Yasuoka, Y. Ihara, S. Ikeda, Y. Miyahara, T. Kondo, and S. Kohno Antiapoptotic Activity of Akt Is Down-regulated by Ca2+ in Myocardiac H9c2 Cells: EVIDENCE OF Ca2+-DEPENDENT REGULATION OF PROTEIN PHOSPHATASE 2Ac J. Biol. Chem., December 3, 2004; 279(49): 51182 - 51192. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. C. Gupta, S. Mishra, S. Rastogi, M. Imai, O. Habib, and H. N. Sabbah Cardiac SR-coupled PP1 activity and expression are increased and inhibitor 1 protein expression is decreased in failing hearts Am J Physiol Heart Circ Physiol, December 1, 2003; 285(6): H2373 - H2381. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. M. Storey, M. Gomez-Angelats, C. D. Bortner, D. L. Armstrong, and J. A. Cidlowski Stimulation of Kv1.3 Potassium Channels by Death Receptors during Apoptosis in Jurkat T Lymphocytes J. Biol. Chem., August 29, 2003; 278(35): 33319 - 33326. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Esposti, G. Ferry, P. Masdehors, J. A. Boutin, J. A. Hickman, and C. Dive Post-translational Modification of Bid Has Differential Effects on Its Susceptibility to Cleavage by Caspase 8 or Caspase 3 J. Biol. Chem., April 25, 2003; 278(18): 15749 - 15757. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Bennin, A. S. A. Don, T. Brake, J. L. McKenzie, H. Rosenbaum, L. Ortiz, A. A. DePaoli-Roach, and M. C. Horne Cyclin G2 Associates with Protein Phosphatase 2A Catalytic and Regulatory B' Subunits in Active Complexes and Induces Nuclear Aberrations and a G1/S Phase Cell Cycle Arrest J. Biol. Chem., July 19, 2002; 277(30): 27449 - 27467. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Silverstein, C. A. Barrow, A. J. Davis, and M. C. Mumby Actions of PP2A on the MAP kinase pathway and apoptosis are mediated by distinct regulatory subunits PNAS, April 2, 2002; 99(7): 4221 - 4226. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. Kolb, S. H. Chang, and M. A. Davis Biochemical and Morphological Events During Okadaic Acid-Induced Apoptosis of Tsc2-Null ERC-18 Cell Line Toxicol Pathol, February 1, 2002; 30(2): 235 - 246. [Abstract] [PDF]
|
|
|
|
|
|
P. J. Beresford, D. Zhang, D. Y. Oh, Z. Fan, E. L. Greer, M. L. Russo, M. Jaju, and J. Lieberman Granzyme A Activates an Endoplasmic Reticulum-associated Caspase-independent Nuclease to Induce Single-stranded DNA Nicks J. Biol. Chem., November 9, 2001; 276(46): 43285 - 43293. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. NEUZIL, T. WEBER, A. SCHRODER, M. LU, G. OSTERMANN, N. GELLERT, G. C. MAYNE, B. OLEJNICKA, A. NEGRE-SALVAYRE, M. STICHA, et al. Induction of cancer cell apoptosis by {alpha}-tocopheryl succinate: molecular pathways and structural requirements FASEB J, February 1, 2001; 15(2): 403 - 415. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Xu and B. R. G. Williams The B56alpha Regulatory Subunit of Protein Phosphatase 2A Is a Target for Regulation by Double-Stranded RNA-Dependent Protein Kinase PKR Mol. Cell. Biol., July 15, 2000; 20(14): 5285 - 5299. [Abstract] [Full Text]
|
|
|
|
|
|
S. S. BAE, D. K. PERRY, Y. S. OH, J. H. CHOI, S. H. GALADARI, T. GHAYUR, S. H. RYU, Y. A. HANNUN, and P.-G. SUH Proteolytic cleavage of phospholipase C-{gamma}1 during apoptosis in Molt-4 cells FASEB J, June 1, 2000; 14(9): 1083 - 1092. [Abstract] [Full Text]
|
|
|
|
 |
|
 P. Lipton Ischemic Cell Death in Brain Neurons Physiol Rev, October 1, 1999; 79(4): 1431 - 1568. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Harris, T. L. Myrick, and S. J. Rundle The Arabidopsis Homolog of Yeast TAP42 and Mammalian alpha 4 Binds to the Catalytic Subunit of Protein Phosphatase 2A and Is Induced by Chilling Plant Physiology, October 1, 1999; 121(2): 609 - 618. [Abstract] [Full Text]
|
|
|
|
|
|
R. Shtrichman, R. Sharf, H. Barr, T. Dobner, and T. Kleinberger Induction of apoptosis by adenovirus E4orf4 protein is specific to transformed cells and requires an interaction with protein phosphatase 2A PNAS, August 31, 1999; 96(18): 10080 - 10085. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Lacomme and S. Santa Cruz Bax-induced cell death in tobacco is similar to the hypersensitive response PNAS, July 6, 1999; 96(14): 7956 - 7961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Walter, A. Schindzielorz, J. Grunberg, and C. Haass Phosphorylation of presenilin-2 regulates its cleavage by caspases and retards progression of apoptosis PNAS, February 16, 1999; 96(4): 1391 - 1396. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. K. MILLER Activation of Apoptosis and Its Inhibition Ann. N.Y. Acad. Sci., January 1, 1999; 886(1): 132 - 157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Mao, S. D. Desai, C.-Y. Ting, J. Hwang, and L. F. Liu 26 S Proteasome-mediated Degradation of Topoisomerase II Cleavable Complexes J. Biol. Chem., October 26, 2001; 276(44): 40652 - 40658. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Silverstein, C. A. Barrow, A. J. Davis, and M. C. Mumby Actions of PP2A on the MAP kinase pathway and apoptosis are mediated by distinct regulatory subunits PNAS, April 2, 2002; 99(7): 4221 - 4226. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
