HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Jung, S. M. Articles by Moroi, M. Search for Related Content PubMed PubMed Citation Articles by Jung, S. M. Articles by Moroi, M. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 24, 14827-14837, June 12, 1998

Platelets Interact with Soluble and Insoluble Collagens through Characteristically Different Reactions

From the Department of Protein Biochemistry, Institute of Life Science, Kurume University, Kurume-shi, Fukuoka-ken 839-8016, Japan

Platelet interaction with soluble and insoluble collagens was characterized through binding studies. In contrast to resting platelets, cells reacted with activators, TS2/16 (integrin ₂₁-activating antibody), thrombin, collagen-related peptide, or ADP, exhibited specific soluble collagen binding that is Mg²⁺-dependent, but inhibited by prostaglandin I₂, Ca²⁺, and Gi9 (anti-integrin ₂₁ antibody). Each platelet has 1500-3500 soluble collagen binding sites, with a dissociation constant of 3.5-9 × 10⁸ M. This is the first study to show the specific binding of soluble collagen to platelets; our data strongly suggest that the receptor is integrin ₂₁ after it becomes activated upon platelet activation. These results suggest that activation of platelets transforms integrin ₂₁ to a state with higher affinity binding sites for soluble collagen. The soluble collagen-platelet interaction was compared with the platelet interaction with fibrillar collagen, which has until now not been demonstrated to bind specifically to platelets. Here, we demonstrated specific, biphasic fibrillar collagen binding. One phase is rapid and metal ion-independent, and accounts for most of the binding. The other phase is slow and Mg²⁺-dependent. The characteristic differences in the specific bindings of soluble and fibrous collagens demonstrate the different contributions of two different collagen receptors.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				K. L. Tucker, T. Sage, J. M. Stevens, P. A. Jordan, S. Jones, N. E. Barrett, R. St-Arnaud, J. Frampton, S. Dedhar, and J. M. Gibbins A dual role for integrin-linked kinase in platelets: regulating integrin function and {alpha}-granule secretion Blood, December 1, 2008; 112(12): 4523 - 4531. [Abstract] [Full Text] [PDF]

				L. J. Lambert, A. A. Bobkov, J. W. Smith, and F. M. Marassi Competitive Interactions of Collagen and a Jararhagin-derived Disintegrin Peptide with the Integrin {alpha}2-I Domain J. Biol. Chem., June 13, 2008; 283(24): 16665 - 16672. [Abstract] [Full Text] [PDF]

				S. Yoshida, T. Sudo, M. Niimi, L. Tao, B. Sun, J. Kambayashi, H. Watanabe, E. Luo, and H. Matsuoka Inhibition of collagen-induced platelet aggregation by anopheline antiplatelet protein, a saliva protein from a malaria vector mosquito Blood, February 15, 2008; 111(4): 2007 - 2014. [Abstract] [Full Text] [PDF]

				E. Calvo, F. Tokumasu, O. Marinotti, J.-L. Villeval, J. M. C. Ribeiro, and I. M. B. Francischetti Aegyptin, a Novel Mosquito Salivary Gland Protein, Specifically Binds to Collagen and Prevents Its Interaction with Platelet Glycoprotein VI, Integrin {alpha}2beta1, and von Willebrand Factor J. Biol. Chem., September 14, 2007; 282(37): 26928 - 26938. [Abstract] [Full Text] [PDF]

				G. Bix, R. A. Iozzo, B. Woodall, M. Burrows, A. McQuillan, S. Campbell, G. B. Fields, and R. V. Iozzo Endorepellin, the C-terminal angiostatic module of perlecan, enhances collagen-platelet responses via the {alpha}2{beta}1-integrin receptor Blood, May 1, 2007; 109(9): 3745 - 3748. [Abstract] [Full Text] [PDF]

				B. Bernardi, G. F. Guidetti, F. Campus, J. R. Crittenden, A. M. Graybiel, C. Balduini, and M. Torti The small GTPase Rap1b regulates the cross talk between platelet integrin {alpha}2beta1 and integrin {alpha}IIbbeta3 Blood, April 1, 2006; 107(7): 2728 - 2735. [Abstract] [Full Text] [PDF]

				C. C. Weber, H. Cai, M. Ehrbar, H. Kubota, G. Martiny-Baron, W. Weber, V. Djonov, E. Weber, A. S. Mallik, M. Fussenegger, et al. Effects of Protein and Gene Transfer of the Angiopoietin-1 Fibrinogen-like Receptor-binding Domain on Endothelial and Vessel Organization J. Biol. Chem., June 10, 2005; 280(23): 22445 - 22453. [Abstract] [Full Text] [PDF]

				M. A. Cruz, J. Chen, J. L. Whitelock, L. D. Morales, and J. A. Lopez The platelet glycoprotein Ib-von Willebrand factor interaction activates the collagen receptor {alpha}2{beta}1 to bind collagen: activation-dependent conformational change of the {alpha}2-I domain Blood, March 1, 2005; 105(5): 1986 - 1991. [Abstract] [Full Text] [PDF]

				J. M. Gibbins Platelet adhesion signalling and the regulation of thrombus formation J. Cell Sci., July 15, 2004; 117(16): 3415 - 3425. [Abstract] [Full Text] [PDF]

				A. Schoolmeester, K. Vanhoorelbeke, S. Katsutani, H. Depraetere, H. B. Feys, J. M. W. Heemskerk, M. F. Hoylaerts, and H. Deckmyn Monoclonal antibody IAC-1 is specific for activated {alpha}2{beta}1 and binds to amino acids 199 to 201 of the integrin {alpha}2 I-domain Blood, July 15, 2004; 104(2): 390 - 396. [Abstract] [Full Text] [PDF]

				M. L. Kahn Platelet collagen receptors play molecular ping-pong Blood, February 15, 2004; 103(4): 1180 - 1181. [Full Text] [PDF]

				S. Gruner, M. Prostredna, V. Schulte, T. Krieg, B. Eckes, C. Brakebusch, and B. Nieswandt Multiple integrin-ligand interactions synergize in shear-resistant platelet adhesion at sites of arterial injury in vivo Blood, December 1, 2003; 102(12): 4021 - 4027. [Abstract] [Full Text] [PDF]

				Z. Wang, T. M. Leisner, and L. V. Parise Platelet {alpha}2{beta}1 integrin activation: contribution of ligand internalization and the {alpha}2-cytoplasmic domain Blood, August 15, 2003; 102(4): 1307 - 1315. [Abstract] [Full Text] [PDF]

				H. Chen and M. L. Kahn Reciprocal Signaling by Integrin and Nonintegrin Receptors during Collagen Activation of Platelets Mol. Cell. Biol., July 15, 2003; 23(14): 4764 - 4777. [Abstract] [Full Text] [PDF]

				B. Nieswandt and S. P. Watson Platelet-collagen interaction: is GPVI the central receptor? Blood, July 15, 2003; 102(2): 449 - 461. [Abstract] [Full Text] [PDF]

				N. Watanabe, H. Nakajima, H. Suzuki, A. Oda, Y. Matsubara, M. Moroi, Y. Terauchi, T. Kadowaki, H. Suzuki, S. Koyasu, et al. Functional phenotype of phosphoinositide 3-kinase p85{alpha}-null platelets characterized by an impaired response to GP VI stimulation Blood, July 15, 2003; 102(2): 541 - 548. [Abstract] [Full Text] [PDF]

				O. Inoue, K. Suzuki-Inoue, W. L. Dean, J. Frampton, and S. P. Watson Integrin {alpha}2{beta}1 mediates outside-in regulation of platelet spreading on collagen through activation of Src kinases and PLC{gamma}2 J. Cell Biol., March 3, 2003; 160(5): 769 - 780. [Abstract] [Full Text] [PDF]

				Y. Miura, T. Takahashi, S. M. Jung, and M. Moroi Analysis of the Interaction of Platelet Collagen Receptor Glycoprotein VI (GPVI) with Collagen. A DIMERIC FORM OF GPVI, BUT NOT THE MONOMERIC FORM, SHOWS AFFINITY TO FIBROUS COLLAGEN J. Biol. Chem., November 22, 2002; 277(48): 46197 - 46204. [Abstract] [Full Text] [PDF]

				G. Guidetti, A. Bertoni, M. Viola, E. Tira, C. Balduini, and M. Torti The small proteoglycan decorin supports adhesion and activation of human platelets Blood, August 13, 2002; 100(5): 1707 - 1714. [Abstract] [Full Text] [PDF]

				T. J. Kunicki The Influence of Platelet Collagen Receptor Polymorphisms in Hemostasis and Thrombotic Disease Arterioscler. Thromb. Vasc. Biol., January 1, 2002; 22(1): 14 - 20. [Abstract] [Full Text] [PDF]

				S. M. Jung and M. Moroi Signal-transducing Mechanisms Involved in Activation of the Platelet Collagen Receptor Integrin alpha 2beta 1 J. Biol. Chem., March 10, 2000; 275(11): 8016 - 8026. [Abstract] [Full Text] [PDF]

				B. Savage, M. H. Ginsberg, and Z. M. Ruggeri Influence of Fibrillar Collagen Structure on the Mechanisms of Platelet Thrombus Formation Under Flow Blood, October 15, 1999; 94(8): 2704 - 2715. [Abstract] [Full Text] [PDF]

				J. Chung, X.-Q. Wang, F. P. Lindberg, and W. A. Frazier Thrombospondin-1 Acts Via IAP/CD47 to Synergize With Collagen in alpha 2beta 1-Mediated Platelet Activation Blood, July 15, 1999; 94(2): 642 - 648. [Abstract] [Full Text] [PDF]

				K. Suzuki-Inoue, Y. Ozaki, M. Kainoh, Y. Shin, Y. Wu, Y. Yatomi, T. Ohmori, T. Tanaka, K. Satoh, and T. Morita Rhodocytin Induces Platelet Aggregation by Interacting with Glycoprotein Ia/IIa (GPIa/IIa, Integrin alpha 2beta 1). INVOLVEMENT OF GPIa/IIa-ASSOCIATED Src AND PROTEIN TYROSINE PHOSPHORYLATION J. Biol. Chem., January 5, 2001; 276(2): 1643 - 1652. [Abstract] [Full Text] [PDF]

				J. A. Eble, B. Beermann, H.-J. Hinz, and A. Schmidt-Hederich alpha 2beta 1 Integrin Is Not Recognized by Rhodocytin but Is the Specific, High Affinity Target of Rhodocetin, an RGD-independent Disintegrin and Potent Inhibitor of Cell Adhesion to Collagen J. Biol. Chem., April 6, 2001; 276(15): 12274 - 12284. [Abstract] [Full Text] [PDF]

				T. R. Carlson, Y. Feng, P. C. Maisonpierre, M. Mrksich, and A. O. Morla Direct Cell Adhesion to the Angiopoietins Mediated by Integrins J. Biol. Chem., July 6, 2001; 276(28): 26516 - 26525. [Abstract] [Full Text] [PDF]

				A. Navdaev, J. M. Clemetson, J. Polgar, B. E. Kehrel, M. Glauner, E. Magnenat, T. N. C. Wells, and K. J. Clemetson Aggretin, a Heterodimeric C-type Lectin from Calloselasma rhodostoma (Malayan Pit Viper), Stimulates Platelets by Binding to alpha 2beta 1 Integrin and Glycoprotein Ib, Activating Syk and Phospholipase Cgamma 2, but Does Not Involve the Glycoprotein VI/Fc Receptor gamma Chain Collagen Receptor J. Biol. Chem., June 8, 2001; 276(24): 20882 - 20889. [Abstract] [Full Text] [PDF]