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J Biol Chem, Vol. 273, Issue 24, 15131-15137, June 12, 1998
From the Department of Biochemistry, Sciences II, University of
Geneva, 1211 Geneva 4, Switzerland
Within the chick central nervous system,
expression of the
Functional Properties of the Neuronal Nicotinic Acetylcholine
Receptor
3 Promoter in the Developing Central Nervous System
3 nicotinic acetylcholine receptor gene is
restricted to a subset of retinal neurons, the majority of which are
ganglion cells. Transient transfection in retinal neurons and in neural
and non-neural cells from other regions of the chick embryo allowed the
identification of the cis-regulatory domain of the 3 gene. Within
this domain, a 75-base pair fragment located immediately upstream of
the transcription start site suffices to reproduce the neuron-specific
expression pattern of 3. This fragment encompasses an E-box and a
CAAT box, both of which are shown to be key positive regulatory
elements of the 3 promoter. Co-transfection experiments into
retinal, telencephalic, and tectal neurons with plasmid reporters of
3 promoter activity and a number of vectors expressing different neuronal (ASH-1, NeuroM, NeuroD, CTF-4) and non-neuronal (MyoD) basic
helix-loop-helix transcription factors indicate that the cis-regulatory
domain of 3 has the remarkable property of discriminating accurately
between related members of the basic helix-loop-helix protein family.
The sequence located immediately 3' of the E-box participates in this
selection, and the E-box acts in concert with the nearby CAAT box.
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
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