HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ferreira, M. C. d. J. Articles by Chabardès, D. Search for Related Content PubMed PubMed Citation Articles by Ferreira, M. C. d. J. Articles by Chabardès, D. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 24, 15192-15202, June 12, 1998

Co-expression of a Ca²⁺-inhibitable Adenylyl Cyclase and of a Ca²⁺-sensing Receptor in the Cortical Thick Ascending Limb Cell of the Rat Kidney
INHIBITION OF HORMONE-DEPENDENT cAMP ACCUMULATION BY EXTRACELLULAR Ca²⁺

From the Service de Biologie Cellulaire, Département de Biologie Cellulaire et Moléculaire, CEA Saclay, France

The Ca²⁺-sensing receptor protein and the Ca²⁺-inhibitable type 6 adenylyl cyclase mRNA are present in a defined segment of the rat renal tubule leading to the hypothesis of their possible functional co-expression in a same cell and thus to a possible inhibition of cAMP content by extracellular Ca²⁺. By using microdissected segments, we compared the properties of regulation of extracellular Ca²⁺-mediated activation of Ca²⁺ receptor to those elicited by prostaglandin E₂ and angiotensin II. The three agents inhibited a common pool of hormone-stimulated cAMP content by different mechanisms as follows. (i) Extracellular Ca²⁺, coupled to phospholipase C activation via a pertussis toxin-insensitive G protein, induced a dose-dependent inhibition of cAMP content (1.25 mM Ca²⁺ eliciting 50% inhibition) resulting from both stimulation of cAMP hydrolysis and inhibition of cAMP synthesis; this latter effect was mediated by capacitive Ca²⁺ influx as well as release of intracellular Ca²⁺. (ii) Angiotensin II, coupled to the same transduction pathway, also decreased cAMP content; however, its inhibitory effect on cAMP was mainly accounted for by an increase of cAMP hydrolysis, although angiotensin II and extracellular Ca²⁺ can induce comparable release of intracellular Ca²⁺. (iii) Prostaglandin E₂, coupled to pertussis toxin-sensitive G protein, inhibited the same pool of adenylyl cyclase units as extracellular Ca²⁺ but by a different mechanism. The functional properties of the adenylyl cyclase were similar to those described for type 6. The results establish that the co-expression of a Ca²⁺-inhibitable adenylyl cyclase and of a Ca²⁺-sensing receptor in a same cell allows an inhibition of cAMP accumulation by physiological concentrations of extracellular Ca²⁺.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				H. I. Abdullah, P. L. Pedraza, J. C. McGiff, and N. R. Ferreri Calcium-sensing receptor signaling pathways in medullary thick ascending limb cells mediate COX-2-derived PGE2 production: functional significance Am J Physiol Renal Physiol, October 1, 2008; 295(4): F1082 - F1089. [Abstract] [Full Text] [PDF]

				C.-L. Tu, W. Chang, Z. Xie, and D. D. Bikle Inactivation of the Calcium Sensing Receptor Inhibits E-cadherin-mediated Cell-Cell Adhesion and Calcium-induced Differentiation in Human Epidermal Keratinocytes J. Biol. Chem., February 8, 2008; 283(6): 3519 - 3528. [Abstract] [Full Text] [PDF]

				M. Bustamante, U. Hasler, V. Leroy, S. de Seigneux, M. Dimitrov, D. Mordasini, M. Rousselot, P.-Y. Martin, and E. Feraille Calcium-sensing Receptor Attenuates AVP-induced Aquaporin-2 Expression via a Calmodulin-dependent Mechanism J. Am. Soc. Nephrol., January 1, 2008; 19(1): 109 - 116. [Abstract] [Full Text] [PDF]

				J. Geibel, K. Sritharan, R. Geibel, P. Geibel, J. S. Persing, A. Seeger, T. K. Roepke, M. Deichstetter, C. Prinz, S. X. Cheng, et al. Inaugural Article: Calcium-sensing receptor abrogates secretagogue- induced increases in intestinal net fluid secretion by enhancing cyclic nucleotide destruction PNAS, June 20, 2006; 103(25): 9390 - 9397. [Abstract] [Full Text] [PDF]

				Y. Hibi, F. Kambe, Y. Tominaga, Y. Mizuno, H. Kobayashi, K. Iwase, T. Imai, and H. Seo Up-Regulation of the Gene Encoding Protein Kinase A Type I{alpha} Regulatory Subunit in Nodular Hyperplasia of Parathyroid Glands in Patients with Chronic Renal Failure J. Clin. Endocrinol. Metab., February 1, 2006; 91(2): 563 - 568. [Abstract] [Full Text] [PDF]

				A. Gerbino, W. C. Ruder, S. Curci, T. Pozzan, M. Zaccolo, and A. M. Hofer Termination of cAMP signals by Ca2+ and G{alpha}i via extracellular Ca2+ sensors: a link to intracellular Ca2+ oscillations J. Cell Biol., October 24, 2005; 171(2): 303 - 312. [Abstract] [Full Text] [PDF]

				M. Xu, S. Choudhary, D. Goltzman, F. Ledgard, D. Adams, G. Gronowicz, B. Koczon-Jaremko, L. Raisz, and C. Pilbeam Do Cyclooxygenase-2 Knockout Mice Have Primary Hyperparathyroidism? Endocrinology, April 1, 2005; 146(4): 1843 - 1853. [Abstract] [Full Text] [PDF]

				S. C. Hebert, G. Desir, G. Giebisch, and W. Wang Molecular Diversity and Regulation of Renal Potassium Channels Physiol Rev, January 1, 2005; 85(1): 319 - 371. [Abstract] [Full Text] [PDF]

				W. Wang, C. Li, T.-H. Kwon, R. T. Miller, M. A. Knepper, J. Frokiaer, and S. Nielsen Reduced expression of renal Na+ transporters in rats with PTH-induced hypercalcemia Am J Physiol Renal Physiol, March 1, 2004; 286(3): F534 - F545. [Abstract] [Full Text]

				C. Huang, K. M. Hujer, Z. Wu, and R. T. Miller The Ca2+-sensing receptor couples to G{alpha}12/13 to activate phospholipase D in Madin-Darby canine kidney cells Am J Physiol Cell Physiol, January 1, 2004; 286(1): C22 - C30. [Abstract] [Full Text] [PDF]

				P. Houillier and M. Paillard Calcium-sensing receptor and renal cation handling Nephrol. Dial. Transplant., December 1, 2003; 18(12): 2467 - 2470. [Full Text] [PDF]

				N. Laroche-Joubert, S. Marsy, S. Luriau, M. Imbert-Teboul, and A. Doucet Mechanism of activation of ERK and H-K-ATPase by isoproterenol in rat cortical collecting duct Am J Physiol Renal Physiol, May 1, 2003; 284(5): F948 - F954. [Abstract] [Full Text] [PDF]

				H. I. Motoyama and P. A. Friedman Calcium-sensing receptor regulation of PTH-dependent calcium absorption by mouse cortical ascending limbs Am J Physiol Renal Physiol, September 1, 2002; 283(3): F399 - F406. [Abstract] [Full Text] [PDF]

				S. K. Fellner and L. Parker A Ca2+-sensing receptor modulates shark rectal gland function J. Exp. Biol., July 1, 2002; 205(13): 1889 - 1897. [Abstract] [Full Text] [PDF]

				S. X. Cheng, M. Okuda, A. E. Hall, J. P. Geibel, and S. C. Hebert Expression of calcium-sensing receptor in rat colonic epithelium: evidence for modulation of fluid secretion Am J Physiol Gastrointest Liver Physiol, July 1, 2002; 283(1): G240 - G250. [Abstract] [Full Text] [PDF]

				C. Huang, M. E. Handlogten, and R. T. Miller Parallel Activation of Phosphatidylinositol 4-Kinase and Phospholipase C by the Extracellular Calcium-sensing Receptor J. Biol. Chem., May 31, 2002; 277(23): 20293 - 20300. [Abstract] [Full Text] [PDF]

				M. A. BAILEY, M. IMBERT-TEBOUL, C. TURNER, S. K. SRAI, G. BURNSTOCK, and R. J. UNWIN Evidence for Basolateral P2Y6 Receptors along the Rat Proximal Tubule: Functional and Molecular Characterization J. Am. Soc. Nephrol., August 1, 2001; 12(8): 1640 - 1647. [Abstract] [Full Text] [PDF]

				E. M. Brown and R. J. MacLeod Extracellular Calcium Sensing and Extracellular Calcium Signaling Physiol Rev, January 1, 2001; 81(1): 239 - 297. [Abstract] [Full Text] [PDF]

				E. Feraille and A. Doucet Sodium-Potassium-Adenosinetriphosphatase-Dependent Sodium Transport in the Kidney: Hormonal Control Physiol Rev, January 1, 2001; 81(1): 345 - 418. [Abstract] [Full Text] [PDF]

				N. Defer, M. Best-Belpomme, and J. Hanoune Tissue specificity and physiological relevance of various isoforms of adenylyl cyclase Am J Physiol Renal Physiol, September 1, 2000; 279(3): F400 - F416. [Abstract] [Full Text] [PDF]

				C. Helies-Toussaint, L. Aarab, J.-M. Gasc, J.-M. Verbavatz, and D. Chabardes Cellular localization of type 5 and type 6 ACs in collecting duct and regulation of cAMP synthesis Am J Physiol Renal Physiol, July 1, 2000; 279(1): F185 - F194. [Abstract] [Full Text] [PDF]

				W. Chang, C. Tu, T.-H. Chen, L. Komuves, Y. Oda, S. A. Pratt, S. Miller, and D. Shoback Expression and Signal Transduction of Calcium-Sensing Receptors in Cartilage and Bone Endocrinology, December 1, 1999; 140(12): 5883 - 5893. [Abstract] [Full Text]