HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nakamura, H. Articles by Utiyama, H. Search for Related Content PubMed PubMed Citation Articles by Nakamura, H. Articles by Utiyama, H. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 25, 15345-15351, June 19, 1998

Survival by Mac-1-mediated Adherence and Anoikis in Phorbol Ester-treated HL-60 Cells

Hitoshi Nakamura, Tsukasa Oda, Koichi Hamada, Tetsuo Hirano, Noriaki Shimizu, and Hiroyasu Utiyama

From the Life Science Group, Faculty of Integrated Arts and Sciences, Hiroshima University, Kagamiyama 1-7-1, Higashi-Hiroshima 739-8521 and the  Helix Research Institute, Yana 1532-3, Kisarazu 292-0812, Japan

During the exposure of human myelocytic leukemia HL-60 cells to phorbol diester, nonadherent cells die by apoptosis, but adherent cells survive and growth-arrest at G₁ phase of the cell cycle. Here we have shown that the adherent cells rapidly died by apoptosis after forced detachment (anoikis), indicating that phorbol diester induced apoptosis by default. Dimethylsphingosine induced apoptosis in the adherent cells, and sphingosine-1-phosphate rescued the detached cells from apoptosis. Sphingosine kinase activity in adherent cells was higher than that in nonadherent cells and was decreased by forced detachment. It is likely that the phorbol diester-induced apoptosis and the adhesion-mediated survival are modulated by sphingosine and sphingosine-1-phosphate, respectively. The adherent cells were reverted and reproliferated when allowed to spontaneously detach from plastic surfaces by removal of phorbol diester. This result suggests that after removal of phorbol diester, the commitment signal of apoptosis by default is lost faster than the survival signal by adherence.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				K Anttonen, A Orpana, M Leirisalo-Repo, and H Repo Aberrant TNF secretion by whole blood in healthy subjects with a history of reactive arthritis: time course in adherent and non-adherent cultures Ann Rheum Dis, March 1, 2006; 65(3): 372 - 378. [Abstract] [Full Text] [PDF]

				U. Steidl, R. Kronenwett, U.-P. Rohr, R. Fenk, S. Kliszewski, C. Maercker, P. Neubert, M. Aivado, J. Koch, O. Modlich, et al. Gene expression profiling identifies significant differences between the molecular phenotypes of bone marrow-derived and circulating human CD34+ hematopoietic stem cells Blood, March 15, 2002; 99(6): 2037 - 2044. [Abstract] [Full Text] [PDF]

				O. Cuvillier and T. Levade Sphingosine 1-phosphate antagonizes apoptosis of human leukemia cells by inhibiting release of cytochrome c and Smac/DIABLO from mitochondria Blood, November 1, 2001; 98(9): 2828 - 2836. [Abstract] [Full Text] [PDF]

				Z. Zhu, O. Sanchez-Sweatman, X. Huang, R. Wiltrout, R. Khokha, Q. Zhao, and E. Gorelik Anoikis and Metastatic Potential of Cloudman S91 Melanoma Cells Cancer Res., February 1, 2001; 61(4): 1707 - 1716. [Abstract] [Full Text]

				A. E. Li, H. Ito, I. I. Rovira, K.-S. Kim, K. Takeda, Z.-Y. Yu, V. J. Ferrans, and T. Finkel A Role for Reactive Oxygen Species in Endothelial Cell Anoikis Circ. Res., August 20, 1999; 85(4): 304 - 310. [Abstract] [Full Text] [PDF]