| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 25, 15382-15386, June 19, 1998
,
From In vitro studies have shown that
ferritin iron incorporation is mediated by a ferroxidase activity
associated with ferritin H subunits (H-Ft) and a nucleation center
associated with ferritin L subunits (L-Ft). To assess the role played
by the ferritin subunits in regulating intracellular iron distribution,
we transfected mouse erythroleukemia cells with the H-Ft subunit gene
mutated in the iron-responsive element. Stable transfectants displayed high H-Ft levels and reduced endogenous L-Ft levels, resulting in a
marked change in the H:L subunit ratio from 1:1 in control cells to as
high as 20:1 in some transfected clones. The effects of H-Ft
overexpression on the labile iron pool were determined in intact cells
by a novel method based on the fluorescent metallosensor calcein. H-Ft
overexpression resulted in a significant reduction in the iron pool,
from 1.3 µM in control cells to 0.56 µM in
H-Ft transfectants, and in higher buffering capacity following iron loads. A fraction of the H-Ft-associated iron was labile, available to
cell-permeant, but not cell-impermeant, chelators. The results of this
study provide the first in vivo direct demonstration of the
capacity of H-Ft to sequester cell iron and to regulate the levels of
the labile iron pool.
INSERM U409, Faculté Xavier Bichat, BP416,
75870 Paris Cedex 18, France, the § Department of Biological
Chemistry, Institute of Life Sciences, Hebrew University of Jerusalem,
Jerusalem 91904, Israel, and ¶ Dipartimento di Ricerca
Biologica e Tecnologica, San Raffaele Scientific Institute, Via
Olgettina 58, 20132 Milano, Italy
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  B. J. Niles, M. S. Clegg, L. A. Hanna, S. S. Chou, T. Y. Momma, H. Hong, and C. L. Keen Zinc Deficiency-induced Iron Accumulation, a Consequence of Alterations in Iron Regulatory Protein-binding Activity, Iron Transporters, and Iron Storage Proteins J. Biol. Chem., February 22, 2008; 283(8): 5168 - 5177. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Shvartsman, R. Kikkeri, A. Shanzer, and Z. I. Cabantchik Non-transferrin-bound iron reaches mitochondria by a chelator-inaccessible mechanism: biological and clinical implications Am J Physiol Cell Physiol, October 1, 2007; 293(4): C1383 - C1394. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Goralska, L. N. Fleisher, and M. C. McGahan Ferritin H- and L-Chains in Fiber Cell Canine and Human Lenses of Different Ages Invest. Ophthalmol. Vis. Sci., September 1, 2007; 48(9): 3968 - 3975. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Iwasaki, K. Hailemariam, and Y. Tsuji PIAS3 Interacts with ATF1 and Regulates the Human Ferritin H Gene through an Antioxidant-responsive Element J. Biol. Chem., August 3, 2007; 282(31): 22335 - 22343. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Antosiewicz, A. Herman-Antosiewicz, S. W. Marynowski, and S. V. Singh c-Jun NH2-Terminal Kinase Signaling Axis Regulates Diallyl Trisulfide-Induced Generation of Reactive Oxygen Species and Cell Cycle Arrest in Human Prostate Cancer Cells. Cancer Res., May 15, 2006; 66(10): 5379 - 5386. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Schranzhofer, M. Schifrer, J. A. Cabrera, S. Kopp, P. Chiba, H. Beug, and E. W. Mullner Remodeling the regulation of iron metabolism during erythroid differentiation to ensure efficient heme biosynthesis Blood, May 15, 2006; 107(10): 4159 - 4167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Goralska, S. Nagar, L. N. Fleisher, and M. C. McGahan Differential Degradation of Ferritin H- and L-Chains: Accumulation of L-Chain-Rich Ferritin in Lens Epithelial Cells Invest. Ophthalmol. Vis. Sci., October 1, 2005; 46(10): 3521 - 3529. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Hose, G. Kaur, E. A. Sausville, and A. Monks Transcriptional Profiling Identifies Altered Intracellular Labile Iron Homeostasis as a Contributing Factor to the Toxicity of Adaphostin: Decreased Vascular Endothelial Growth Factor Secretion Is Independent of Hypoxia-Inducible Factor-1 Regulation Clin. Cancer Res., September 1, 2005; 11(17): 6370 - 6381. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Knutson, M. R. Vafa, D. J. Haile, and M. Wessling-Resnick Iron loading and erythrophagocytosis increase ferroportin 1 (FPN1) expression in J774 macrophages Blood, December 1, 2003; 102(12): 4191 - 4197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Goralska, B. L. Holley, and M. C. McGahan Identification of a Mechanism by Which Lens Epithelial Cells Limit Accumulation of Overexpressed Ferritin H-chain J. Biol. Chem., October 31, 2003; 278(44): 42920 - 42926. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. B. Hasinoff, P. E. Schroeder, and D. Patel The Metabolites of the Cardioprotective Drug Dexrazoxane Do Not Protect Myocytes from Doxorubicin-Induced Cytotoxicity Mol. Pharmacol., September 1, 2003; 64(3): 670 - 678. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Corsi, A. Cozzi, P. Arosio, J. Drysdale, P. Santambrogio, A. Campanella, G. Biasiotto, A. Albertini, and S. Levi Human Mitochondrial Ferritin Expressed in HeLa Cells Incorporates Iron and Affects Cellular Iron Metabolism J. Biol. Chem., June 14, 2002; 277(25): 22430 - 22437. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. M. Torti and S. V. Torti Regulation of ferritin genes and protein Blood, May 15, 2002; 99(10): 3505 - 3516. [Full Text] [PDF]
|
|
|
|
|
|
C. Ferreira, P. Santambrogio, M.-E. Martin, V. Andrieu, G. Feldmann, D. Henin, and C. Beaumont H ferritin knockout mice: a model of hyperferritinemia in the absence of iron overload Blood, August 1, 2001; 98(3): 525 - 532. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Goralska, B. L. Holley, and M. C. McGahan Overexpression of H- and L-Ferritin Subunits in Lens Epithelial Cells: Fe Metabolism and Cellular Response to UVB Irradiation Invest. Ophthalmol. Vis. Sci., July 1, 2001; 42(8): 1721 - 1727. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Kakhlon, Y. Gruenbaum, and Z. I. Cabantchik Repression of ferritin expression increases the labile iron pool, oxidative stress, and short-term growth of human erythroleukemia cells Blood, May 1, 2001; 97(9): 2863 - 2871. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Tsuji, H. Ayaki, S. P. Whitman, C. S. Morrow, S. V. Torti, and F. M. Torti Coordinate Transcriptional and Translational Regulation of Ferritin in Response to Oxidative Stress Mol. Cell. Biol., August 15, 2000; 20(16): 5818 - 5827. [Abstract] [Full Text]
|
|
|
|
|
|
P. Lipinski, J.-C. Drapier, L. Oliveira, H. Retmanska, B. Sochanowicz, and M. Kruszewski Intracellular iron status as a hallmark of mammalian cell susceptibility to oxidative stress: a study of L5178Y mouse lymphoma cell lines differentially sensitive to H2O2 Blood, May 1, 2000; 95(9): 2960 - 2966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ferreira, D. Bucchini, M.-E. Martin, S. Levi, P. Arosio, B. Grandchamp, and C. Beaumont Early Embryonic Lethality of H Ferritin Gene Deletion in Mice J. Biol. Chem., February 4, 2000; 275(5): 3021 - 3024. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.-Y. Yeh, M. Yeh, and J. Glass Glucocorticoids and dietary iron regulate postnatal intestinal heavy and light ferritin expression in rats Am J Physiol Gastrointest Liver Physiol, February 1, 2000; 278(2): G217 - G226. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M.C. GUTTERIDGE and B. HALLIWELL Free Radicals and Antioxidants in the Year 2000: A Historical Look to the Future Ann. N.Y. Acad. Sci., January 1, 2000; 899(1): 136 - 147. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Epsztejn, H. Glickstein, V. Picard, I. N. Slotki, W. Breuer, C. Beaumont, and Z. I. Cabantchik H-Ferritin Subunit Overexpression in Erythroid Cells Reduces the Oxidative Stress Response and Induces Multidrug Resistance Properties Blood, November 15, 1999; 94(10): 3593 - 3603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E.G. Meyron-Holtz, B. Vaisman, Z.I. Cabantchik, E. Fibach, T.A. Rouault, C. Hershko, and A.M. Konijn Regulation of Intracellular Iron Metabolism in Human Erythroid Precursors by Internalized Extracellular Ferritin Blood, November 1, 1999; 94(9): 3205 - 3211. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-F. Ma, U. A. Ochsner, M. G. Klotz, V. K. Nanayakkara, M. L. Howell, Z. Johnson, J. E. Posey, M. L. Vasil, J. J. Monaco, and D. J. Hassett Bacterioferritin A Modulates Catalase A (KatA) Activity and Resistance to Hydrogen Peroxide in Pseudomonas aeruginosa J. Bacteriol., June 15, 1999; 181(12): 3730 - 3742. [Abstract] [Full Text]
|
|
|
|
|
|
E. W. Oppenheim, I. M. Nasrallah, M. G. Mastri, and P. J. Stover Mimosine Is a Cell-specific Antagonist of Folate Metabolism J. Biol. Chem., June 16, 2000; 275(25): 19268 - 19274. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Cozzi, B. Corsi, S. Levi, P. Santambrogio, A. Albertini, and P. Arosio Overexpression of Wild Type and Mutated Human Ferritin H-chain in HeLa Cells. IN VIVO ROLE OF FERRITIN FERROXIDASE ACTIVITY J. Biol. Chem., August 11, 2000; 275(33): 25122 - 25129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Picard, G. Govoni, N. Jabado, and P. Gros Nramp 2 (DCT1/DMT1) Expressed at the Plasma Membrane Transports Iron and Other Divalent Cations into a Calcein-accessible Cytoplasmic Pool J. Biol. Chem., November 10, 2000; 275(46): 35738 - 35745. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Suh, E. W. Oppenheim, S. Girgis, and P. J. Stover Purification and Properties of a Folate-catabolizing Enzyme J. Biol. Chem., November 3, 2000; 275(45): 35646 - 35655. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Romeo, L. Christen, E. G. Niles, and D. J. Kosman Intracellular Chelation of Iron by Bipyridyl Inhibits DNA Virus Replication. RIBONUCLEOTIDE REDUCTASE MATURATION AS A PROBE OF INTRACELLULAR IRON POOLS J. Biol. Chem., June 22, 2001; 276(26): 24301 - 24308. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. W. Oppenheim, C. Adelman, X. Liu, and P. J. Stover Heavy Chain Ferritin Enhances Serine Hydroxymethyltransferase Expression and de Novo Thymidine Biosynthesis J. Biol. Chem., June 1, 2001; 276(23): 19855 - 19861. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
