| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 25, 15445-15452, June 19, 1998
,,,, and
From the The Syk family tyrosine kinases play a crucial
role in antigen receptor-mediated signal transduction, but their
regulation and cellular targets remain incompletely defined. Following
receptor engagement, phosphorylation of tyrosine residues within ZAP-70 and Syk is thought to control both kinase activity and recruitment of
modulatory factors. We report here the characterization of novel
mutants of ZAP-70 and Syk, in which conserved C-terminal tyrosine
residues have been replaced by phenylalanines (ZAP YF-C, Syk YF-C).
Both mutant kinases display a prominent gain-of-function phenotype in
Jurkat T cells, as demonstrated by lymphokine promoter activation,
tyrosine phosphorylation of potential targets in vivo, and
elevated intracellular calcium mobilization. While the presence of
p56-Lck was required for ZAP YF-C-induced signaling, Syk YF-C showed
enhanced functional activity in Lck-deficient JCaM1 Jurkat cells. Our
results implicate the C terminus of Syk family kinases as an important
regulatory region modulating T cell activation.
Laboratorium für Molekulare Biologie,
Genzentrum der Universität München, Feodor Lynen Strasse
25, D-81377 München, Germany and the § NIEHS, National
Institutes of Health, Research Triangle Park,
North Carolina 27709
This article has been cited by other articles:
|
|
 |
|
  J. Zhang, E. Berenstein, and R. P. Siraganian Phosphorylation of Tyr342 in the Linker Region of Syk Is Critical for Fc{varepsilon}RI Signaling in Mast Cells Mol. Cell. Biol., December 1, 2002; 22(23): 8144 - 8154. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Lutz, J. Nimpf, M. Jenny, K. Boecklinger, C. Enzinger, G. Utermann, G. Baier-Bitterlich, and G. Baier Evidence of Functional Modulation of the MEKK/JNK/cJun Signaling Cascade by the Low Density Lipoprotein Receptor-related Protein (LRP) J. Biol. Chem., November 1, 2002; 277(45): 43143 - 43151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Sirim, L. Zeitlmann, B. Kellersch, C. S. Falk, D. J. Schendel, and W. Kolanus Calcium Signaling through the beta 2-Cytoplasmic Domain of LFA-1 Requires Intracellular Elements of the T Cell Receptor Complex J. Biol. Chem., November 9, 2001; 276(46): 42945 - 42956. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. S.C. Weber, G. Ostermann, A. Zernecke, A. Schroder, L. B. Klickstein, and C. Weber Dual Role of H-Ras in Regulation of Lymphocyte Function Antigen-1 Activity by Stromal Cell-derived Factor-1alpha : Implications for Leukocyte Transmigration Mol. Biol. Cell, October 1, 2001; 12(10): 3074 - 3086. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. P. Hehner, R. Breitkreutz, G. Shubinsky, H. Unsoeld, K. Schulze-Osthoff, M. L. Schmitz, and W. Droge Enhancement of T Cell Receptor Signaling by a Mild Oxidative Shift in the Intracellular Thiol Pool J. Immunol., October 15, 2000; 165(8): 4319 - 4328. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. B. F. Thien, D. D. L. Bowtell, and W. Y. Langdon Perturbed Regulation of ZAP-70 and Sustained Tyrosine Phosphorylation of LAT and SLP-76 in c-Cbl-Deficient Thymocytes J. Immunol., June 15, 1999; 162(12): 7133 - 7139. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pelosi, V. Di Bartolo, V. Mounier, D. Mege, J.-M. Pascussi, E. Dufour, A. Blondel, and O. Acuto Tyrosine 319 in the Interdomain B of ZAP-70 Is a Binding Site for the Src Homology 2 Domain of Lck J. Biol. Chem., May 14, 1999; 274(20): 14229 - 14237. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. She, M. C. Ruzek, P. Velupillai, I. de Aos, B. Wang, D. A. Harn, J. Sancho, C. A. Biron, and C. Terhorst Generation of antigen-specific cytotoxic T lymphocytes and regulation of cytokine production takes place in the absence of CD3{zeta} Int. Immunol., May 1, 1999; 11(5): 845 - 857. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Di Bartolo, D. Mege, V. Germain, M. Pelosi, E. Dufour, F. Michel, G. Magistrelli, A. Isacchi, and O. Acuto Tyrosine 319, a Newly Identified Phosphorylation Site of ZAP-70, Plays a Critical Role in T Cell Antigen Receptor Signaling J. Biol. Chem., March 5, 1999; 274(10): 6285 - 6294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhang, M. L. Billingsley, R. L. Kincaid, and R. P. Siraganian Phosphorylation of Syk Activation Loop Tyrosines Is Essential for Syk Function. AN IN VIVO STUDY USING A SPECIFIC ANTI-Syk ACTIVATION LOOP PHOSPHOTYROSINE ANTIBODY J. Biol. Chem., November 3, 2000; 275(45): 35442 - 35447. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zeitlmann, P. Sirim, E. Kremmer, and W. Kolanus Cloning of ACP33 as a Novel Intracellular Ligand of CD4 J. Biol. Chem., March 16, 2001; 276(12): 9123 - 9132. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
