HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) An addition or correction has been published Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Liberati, C. Articles by Mantovani, R. Search for Related Content PubMed PubMed Citation Articles by Liberati, C. Articles by Mantovani, R. Social Bookmarking                      What's this?

J Biol Chem, Vol. 273, Issue 27, 16880-16889, July 3, 1998

NF-Y Organizes the -Globin CCAAT Boxes Region

From the Dipartimento di Genetica e di Biologia dei Microrganismi, Università di Milano, Via Celoria 26, 20133 Milano, Italy

The CCAAT-binding activator NF-Y is formed by three evolutionary conserved subunits, two of which contain putative histone-like domains. We investigated NF-Y binding to all CCAAT boxes of globin promoters in direct binding, competition, and supershift electrophoretic mobility shift assay; we found that the , , and proximal  CCAAT boxes of human and the prosimian Galago bind avidly, and distal  CCAAT boxes have intermediate affinity, whereas the  and sequences bind NF-Y very poorly. We developed an efficient in vitro transcription system from erythroid K562 cells and established that both the distal and the proximal CCAAT boxes are important for optimal -globin promoter activity. Surprisingly, NF-Y binding to a mutated distal CCAAT box (a C to T at position 114) is remarkably increased upon occupancy of the high affinity proximal element, located 27 base pairs away. Shortening the distance between the two CCAAT boxes progressively prevents simultaneous CCAAT binding, indicating that NF-Y interacts in a mutually exclusive way with CCAAT boxes closer than 24 base pairs apart. A combination of circular permutation and phasing analysis proved that (i) NF-Y-induced angles of the two -globin CCAAT boxes have similar amplitudes; (ii) occupancy of the two CCAAT boxes leads to compensatory distortions; (iii) the two NF-Y bends are spatially oriented with combined twisting angles of about 100°. Interestingly, such distortions are reminiscent of core histone-DNA interactions. We conclude that NF-Y binding imposes a high level of functionally important coordinate organization to the -globin promoter.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				X. Yu, X. Zhu, W. Pi, J. Ling, L. Ko, Y. Takeda, and D. Tuan The Long Terminal Repeat (LTR) of ERV-9 Human Endogenous Retrovirus Binds to NF-Y in the Assembly of an Active LTR Enhancer Complex NF-Y/MZF1/GATA-2 J. Biol. Chem., October 21, 2005; 280(42): 35184 - 35194. [Abstract] [Full Text] [PDF]

				A. R. Osborne, H. Zhang, G. Fejer, K. M. Palubin, M. I. Niesen, and G. Blanck Oct-1 Maintains an Intermediate, Stable State of HLA-DRA Promoter Repression in Rb-defective Cells: AN Oct-1-CONTAINING REPRESSOSOME THAT PREVENTS NF-Y BINDING TO THE HLA-DRA PROMOTER J. Biol. Chem., July 9, 2004; 279(28): 28911 - 28919. [Abstract] [Full Text] [PDF]

				X. Fang, H. Han, G. Stamatoyannopoulos, and Q. Li Developmentally Specific Role of the CCAAT Box in Regulation of Human {gamma}-Globin Gene Expression J. Biol. Chem., February 13, 2004; 279(7): 5444 - 5449. [Abstract] [Full Text] [PDF]

				D. Coss, S. B. R. Jacobs, C. E. Bender, and P. L. Mellon A Novel AP-1 Site Is Critical for Maximal Induction of the Follicle-stimulating Hormone {beta} Gene by Gonadotropin-releasing Hormone J. Biol. Chem., January 2, 2004; 279(1): 152 - 162. [Abstract] [Full Text] [PDF]

				V. Salsi, G. Caretti, M. Wasner, W. Reinhard, U. Haugwitz, K. Engeland, and R. Mantovani Interactions between p300 and Multiple NF-Y Trimers Govern Cyclin B2 Promoter Function J. Biol. Chem., February 21, 2003; 278(9): 6642 - 6650. [Abstract] [Full Text] [PDF]

				P. M. Gowri, J. H. Yu, A. Shaufl, M. A. Sperling, and R. K. Menon Recruitment of a Repressosome Complex at the Growth Hormone Receptor Promoter and Its Potential Role in Diabetic Nephropathy Mol. Cell. Biol., February 1, 2003; 23(3): 815 - 825. [Abstract] [Full Text]

				C. Romier, F. Cocchiarella, R. Mantovani, and D. Moras The NF-YB/NF-YC Structure Gives Insight into DNA Binding and Transcription Regulation by CCAAT Factor NF-Y J. Biol. Chem., January 3, 2003; 278(2): 1336 - 1345. [Abstract] [Full Text] [PDF]

				C. Liberati, M. R. Cera, P. Secco, C. Santoro, R. Mantovani, S. Ottolenghi, and A. Ronchi Cooperation and Competition between the Binding of COUP-TFII and NF-Y on Human epsilon - and gamma -Globin Gene Promoters J. Biol. Chem., November 2, 2001; 276(45): 41700 - 41709. [Abstract] [Full Text] [PDF]

				Z. Duan, G. Stamatoyannopoulos, and Q. Li Role of NF-Y in In Vivo Regulation of the {gamma}-Globin Gene Mol. Cell. Biol., May 1, 2001; 21(9): 3083 - 3095. [Abstract] [Full Text]

				M. Minuzzo, S. Marchini, M. Broggini, G. Faircloth, M. D'Incalci, and R. Mantovani Interference of transcriptional activation by the antineoplastic drug ecteinascidin-743 PNAS, June 6, 2000; 97(12): 6780 - 6784. [Abstract] [Full Text] [PDF]

				G. Caretti, M. C. Motta, and R. Mantovani NF-Y Associates with H3-H4 Tetramers and Octamers by Multiple Mechanisms Mol. Cell. Biol., December 1, 1999; 19(12): 8591 - 8603. [Abstract] [Full Text] [PDF]

				M. C. Motta, G. Caretti, G. F. Badaracco, and R. Mantovani Interactions of the CCAAT-binding Trimer NF-Y with Nucleosomes J. Biol. Chem., January 15, 1999; 274(3): 1326 - 1333. [Abstract] [Full Text] [PDF]

				K. Korner and R. Muller In Vivo Structure of the Cell Cycle-regulated Human cdc25C Promoter J. Biol. Chem., June 16, 2000; 275(25): 18676 - 18681. [Abstract] [Full Text] [PDF]

				F. Liang, F. Schaufele, and D. G. Gardner Functional Interaction of NF-Y and Sp1 Is Required for Type A Natriuretic Peptide Receptor Gene Transcription J. Biol. Chem., January 5, 2001; 276(2): 1516 - 1522. [Abstract] [Full Text] [PDF]