|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 28, 17361-17367, July 10, 1998
From the Divisions of To delineate the regulation of the human
Multiple
-Promoter Elements Participate in the Developmental
Control of -Globin Genes in Transgenic Mice
,, and
Medical Genetics and
§ Hematology, University of Washington, Seattle, Washington
98195 and ¶ Bristol-Myers Squibb Pharmaceutical Research
Institutes, Seattle, Washington 98121
-globin gene, we investigated -gene expression during the
development of transgenic mice carrying constructs with -promoter
truncations linked to a micro-locus control region (µLCR). Expression
levels were compared with those of µLCR mice carrying a 2 kilobase
-promoter and 
YAC controls. mRNA in the embryonic cells
of µLCR (
179) mice were as high as in µLCR mice suggesting
that the proximal -promoter contains most elements required for
-gene activation. mRNA in adult µLCR (179) mice was
significantly lower than in the embryonic cells indicating that
elements involved in -gene silencing are contained in the proximal
-promoter. Extension of the promoter sequence to 463 decreased
-gene expression in the definitive erythroid cells, supporting
previous evidence that the 179 to 463 region contains an
-gene silencer. However, the -gene of the µLCR(463) mice
was not silenced in the definitive cells of fetal and adult
erythropoiesis indicating that additional silencing elements are
located upstream of position 463. These results provide in
vivo evidence that multiple elements of the distal as well as the
proximal promoter contribute to -gene silencing.
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  P. A. Navas, Q. Li, K. R. Peterson, and G. Stamatoyannopoulos Investigations of a Human Embryonic Globin Gene Silencing Element Using YAC Transgenic Mice. Experimental Biology and Medicine, March 1, 2006; 231(3): 328 - 334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Harju, K. J. McQueen, and K. R. Peterson Chromatin Structure and Control of {beta}-Like Globin Gene Switching Experimental Biology and Medicine, October 1, 2002; 227(9): 683 - 700. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. A. Foley, S. F. Ofori-Acquah, A. Yoshimura, S. Critz, B. S. Baliga, and B. S. Pace Stat3beta Inhibits gamma -Globin Gene Expression in Erythroid Cells J. Biol. Chem., May 3, 2002; 277(18): 16211 - 16219. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Stone and G. A. Wray Rapid Evolution of cis-Regulatory Sequences via Local Point Mutations Mol. Biol. Evol., September 1, 2001; 18(9): 1764 - 1770. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Tanimoto, Q. Liu, F. Grosveld, J. Bungert, and J. D. Engel Context-dependent EKLF responsiveness defines the developmental specificity of the human varepsilon -globin gene in erythroid cells of YAC transgenic mice Genes & Dev., November 1, 2000; 14(21): 2778 - 2794. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. Skarpidi, G. Vassilopoulos, Q. Li, and G. Stamatoyannopoulos Novel in vitro assay for the detection of pharmacologic inducers of fetal hemoglobin Blood, July 1, 2000; 96(1): 321 - 326. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Papayannopoulou, G. V. Priestley, A. Rohde, K. R. Peterson, and B. Nakamoto Hemopoietic lineage commitment decisions: in vivo evidence from a transgenic mouse model harboring {micro}LCR-beta pro-LacZ as a transgene Blood, February 15, 2000; 95(4): 1274 - 1282. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |