| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 28, 17386-17390, July 10, 1998
From the Immunology Program, H. Lee Moffitt Cancer Center and
Research Institute, and the Department of Biochemistry and Molecular
Biology, University of South Florida College of Medicine,
Tampa, Florida 33612
Chemical cross-linking of the transporter
associated with antigen processing (TAP) heterodimer was used to
determine whether the herpes simplex virus inhibitor of TAP, ICP47,
induces a conformational change in TAP. Cross-linking of TAP in
cellular membranes produced a major species of ~220 kDa which was
comprised solely of TAP.1 and TAP.2 and most likely represents the TAP
heterodimer. Interestingly, prior treatment of TAP-containing membranes
with TAP peptide substrates stimulated the formation of the
cross-linked TAP heterodimer, whereas pretreatment of membranes with
ICP47 completely blocked the formation of the cross-linked heterodimer.
These data suggest that suitable substrates for TAP stabilize the TAP
heterodimer, whereas ICP47 destabilizes the heterodimer. The results
indicate that subtle conformational changes occur in the TAP
heterodimer upon the binding of peptides and the inhibitor ICP47 and
that ICP47 has a deleterious effect on TAP heterodimer structure, in addition to its role as a potent blocker of substrate binding to
TAP.
This article has been cited by other articles:
|
|
 |
|
  O. Demirel, Z. Waibler, U. Kalinke, F. Grunebach, S. Appel, P. Brossart, A. Hasilik, R. Tampe, and R. Abele Identification of a Lysosomal Peptide Transport System Induced during Dendritic Cell Development J. Biol. Chem., December 28, 2007; 282(52): 37836 - 37843. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ehses, R. M. Leonhardt, G. Hansen, and M. R. Knittler Functional Role of C-Terminal Sequence Elements in the Transporter Associated with Antigen Processing J. Immunol., January 1, 2005; 174(1): 328 - 339. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Abele and R. Tampe The ABCs of Immunology: Structure and Function of TAP, the Transporter Associated with Antigen Processing Physiology, August 1, 2004; 19(4): 216 - 224. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ohsawa, D. H. Black, H. Sato, and R. Eberle Sequence and Genetic Arrangement of the US Region of the Monkey B Virus (Cercopithecine Herpesvirus 1) Genome and Comparison with the US Regions of Other Primate Herpesviruses J. Virol., February 1, 2002; 76(3): 1516 - 1520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Velarde, R. C. Ford, M. F. Rosenberg, and S. J. Powis Three-dimensional Structure of Transporter Associated with Antigen Processing (TAP) Obtained by Single Particle Image Analysis J. Biol. Chem., November 30, 2001; 276(49): 46054 - 46063. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. T. Karttunen, P. J. Lehner, S. S. Gupta, E. W. Hewitt, and P. Cresswell Distinct functions and cooperative interaction of the subunits of the transporter associated with antigen processing (TAP) PNAS, May 24, 2001; (2001) 121180198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gorbulev, R. Abele, and R. Tampe Allosteric crosstalk between peptide-binding, transport, and ATP hydrolysis of the ABC transporter TAP PNAS, March 27, 2001; 98(7): 3732 - 3737. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. van Endert Role of Nucleotides and Peptide Substrate for Stability and Functional State of the Human ABC Family Transporters Associated with Antigen Processing J. Biol. Chem., May 21, 1999; 274(21): 14632 - 14638. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. T. Karttunen, P. J. Lehner, S. S. Gupta, E. W. Hewitt, and P. Cresswell Distinct functions and cooperative interaction of the subunits of the transporter associated with antigen processing (TAP) PNAS, June 19, 2001; 98(13): 7431 - 7436. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
