| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 273, Issue 28, 17504-17510, July 10, 1998
From the Experimental Diabetes, Metabolism, and Nutrition Section,
Diabetes Branch, NIDDK, National Institutes of Health,
Bethesda, Maryland 20892
To study the role of the GTPase dynamin in GLUT4
intracellular recycling, we have overexpressed dynamin
1 wild type and
a GTPase-negative mutant (K44A) in primary rat adipose cells.
Transfection was accomplished by electroporation using an hemagglutinin
(HA)-tagged GLUT4 as a reporter protein. In cells expressing HA-GLUT4
alone, insulin results in an 
7-fold increase in cell surface anti-HA antibody binding. Studies with wortmannin indicate that the kinetics of
HA-GLUT4-trafficking parallel those of the native GLUT4 and in
addition, that newly synthesized HA-GLUT4 goes to the plasma membrane
before being sorted into the insulin-responsive compartments. Short
term (4 h) coexpression of dynamin-K44A and HA-GLUT4 increases the
amount of cell surface HA-GLUT4 in both the basal and
insulin-stimulated states. Under conditions of maximal expression of
dynamin-K44A (24 h), most or all of the intracellular HA-GLUT4 appears
to be present on the cell surface in the basal state, and insulin has no further effect. Measurements of the kinetics of HA-GLUT4 endocytosis show that dynamin-K44A blocks internalization of the glucose
transporters. In contrast, expression of dynamin wild type decreases
the amount of cell surface HA-GLUT4 in both the basal and
insulin-stimulated states. These data demonstrate that the endocytosis
of GLUT4 is largely mediated by processes which require
dynamin.
This article has been cited by other articles:
|
|
 |
|
  M. Larance, G. Ramm, and D. E. James The GLUT4 Code Mol. Endocrinol., February 1, 2008; 22(2): 226 - 233. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Eyster, Q. S. Duggins, G. J. Gorbsky, and A. L. Olson Microtubule Network Is Required for Insulin Signaling through Activation of Akt/Protein Kinase B: EVIDENCE THAT INSULIN STIMULATES VESICLE DOCKING/FUSION BUT NOT INTRACELLULAR MOBILITY J. Biol. Chem., December 22, 2006; 281(51): 39719 - 39727. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Kong, K. A. Horan, A. Sriratana, C. G. Bailey, L. J. Collyer, H. H. Nandurkar, A. Shisheva, M. J. Layton, J. E. J. Rasko, T. Rowe, et al. Phosphatidylinositol 3-Phosphate [PtdIns(3)P] Is Generated at the Plasma Membrane by an Inositol Polyphosphate 5-Phosphatase: Endogenous PtdIns(3)P Can Promote GLUT4 Translocation to the Plasma Membrane. Mol. Cell. Biol., August 1, 2006; 26(16): 6065 - 6081. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Schmidt, S. Briese, K. Leicht, A. Schurmann, H.-G. Joost, and H. Al-Hasani Endocytosis of the glucose transporter GLUT8 is mediated by interaction of a dileucine motif with the {beta}2-adaptin subunit of the AP-2 adaptor complex J. Cell Sci., June 1, 2006; 119(11): 2321 - 2331. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ruehl, U. Erben, D. Schuppan, C. Wagner, A. Zeller, C. Freise, H. Al-Hasani, M. Loesekann, M. Notter, B. M. Wittig, et al. The Elongated First Fibronectin Type III Domain of Collagen XIV Is an Inducer of Quiescence and Differentiation in Fibroblasts and Preadipocytes J. Biol. Chem., November 18, 2005; 280(46): 38537 - 38543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Larance, G. Ramm, J. Stockli, E. M. van Dam, S. Winata, V. Wasinger, F. Simpson, M. Graham, J. R. Junutula, M. Guilhaus, et al. Characterization of the Role of the Rab GTPase-activating Protein AS160 in Insulin-regulated GLUT4 Trafficking J. Biol. Chem., November 11, 2005; 280(45): 37803 - 37813. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Liu, J. C. Hou, R. T. Watson, and J. E. Pessin Initial entry of IRAP into the insulin-responsive storage compartment occurs prior to basal or insulin-stimulated plasma membrane recycling Am J Physiol Endocrinol Metab, November 1, 2005; 289(5): E746 - E752. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. A. Lizunov, H. Matsumoto, J. Zimmerberg, S. W. Cushman, and V. A. Frolov Insulin stimulates the halting, tethering, and fusion of mobile GLUT4 vesicles in rat adipose cells J. Cell Biol., May 9, 2005; 169(3): 481 - 489. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Eyster, Q. S. Duggins, and A. L. Olson Expression of Constitutively Active Akt/Protein Kinase B Signals GLUT4 Translocation in the Absence of an Intact Actin Cytoskeleton J. Biol. Chem., May 6, 2005; 280(18): 17978 - 17985. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Govers, A. C. F. Coster, and D. E. James Insulin Increases Cell Surface GLUT4 Levels by Dose Dependently Discharging GLUT4 into a Cell Surface Recycling Pathway Mol. Cell. Biol., July 15, 2004; 24(14): 6456 - 6466. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. T. Watson, M. Kanzaki, and J. E. Pessin Regulated Membrane Trafficking of the Insulin-Responsive Glucose Transporter 4 in Adipocytes Endocr. Rev., April 1, 2004; 25(2): 177 - 204. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Lu, T.-X. Sun, R. Bouley, K. Blackburn, M. McLaughlin, and D. Brown Inhibition of endocytosis causes phosphorylation (S256)-independent plasma membrane accumulation of AQP2 Am J Physiol Renal Physiol, February 1, 2004; 286(2): F233 - F243. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Olson, C. A. Eyster, Q. S. Duggins, and J. B. Knight Insulin Promotes Formation of Polymerized Microtubules by a Phosphatidylinositol 3-Kinase-Independent, Actin-Dependent Pathway in 3T3-L1 Adipocytes Endocrinology, November 1, 2003; 144(11): 5030 - 5039. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Chen, H. Al-Hasani, T. Olausson, A.-M. Wenthzel, U. Smith, and S. W. Cushman Activity, phosphorylation state and subcellular distribution of GLUT4-targeted Akt2 in rat adipose cells J. Cell Sci., September 1, 2003; 116(17): 3511 - 3518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Shigematsu, R. T. Watson, A. H. Khan, and J. E. Pessin The Adipocyte Plasma Membrane Caveolin Functional/Structural Organization Is Necessary for the Efficient Endocytosis of GLUT4 J. Biol. Chem., March 14, 2003; 278(12): 10683 - 10690. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Konrad, P. J. Bilan, Z. Nawaz, G. Sweeney, W. Niu, Z. Liu, C. N. Antonescu, A. Rudich, and A. Klip Need for GLUT4 Activation to Reach Maximum Effect of Insulin-Mediated Glucose Uptake in Brown Adipocytes Isolated From GLUT4myc-Expressing Mice Diabetes, September 1, 2002; 51(9): 2719 - 2726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yamada, H. Katagiri, T. Asano, M. Tsuru, K. Inukai, H. Ono, T. Kodama, M. Kikuchi, and Y. Oka Role of PDK1 in insulin-signaling pathway for glucose metabolism in 3T3-L1 adipocytes Am J Physiol Endocrinol Metab, June 1, 2002; 282(6): E1385 - E1394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Sterling, D.-H. Lin, R.-M. Gu, K. Dong, S. C. Hebert, and W.-H. Wang Inhibition of Protein-tyrosine Phosphatase Stimulates the Dynamin-dependent Endocytosis of ROMK1 J. Biol. Chem., February 1, 2002; 277(6): 4317 - 4323. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. K. Muday and A. S. Murphy An Emerging Model of Auxin Transport Regulation PLANT CELL, February 1, 2002; 14(2): 293 - 299. [Full Text] [PDF]
|
|
|
|
|
|
H. Al-Hasani, R. K. Kunamneni, K. Dawson, C. S. Hinck, D. Muller-Wieland, and S. W. Cushman Roles of the N- and C-termini of GLUT4 in endocytosis J. Cell Sci., January 1, 2002; 115(1): 131 - 140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Huang, T. Imamura, and J. M. Olefsky Insulin can regulate GLUT4 internalization by signaling to Rab5 and the motor protein dynein PNAS, October 25, 2001; (2001) 241368698. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Shintani, H. Nishimura, S. Yonemitsu, Y. Ogawa, T. Hayashi, K. Hosoda, G. Inoue, and K. Nakao Troglitazone Not Only Increases GLUT4 but Also Induces Its Translocation in Rat Adipocytes Diabetes, October 1, 2001; 50(10): 2296 - 2300. [Abstract] [Full Text]
|
|
|
|
|
|
S. Yonemitsu, H. Nishimura, M. Shintani, R. Inoue, Y. Yamamoto, H. Masuzaki, Y. Ogawa, K. Hosoda, G. Inoue, T. Hayashi, et al. Troglitazone Induces GLUT4 Translocation in L6 Myotubes Diabetes, May 1, 2001; 50(5): 1093 - 1101. [Abstract] [Full Text]
|
|
|
|
|
|
X Zhao, T Greener, H Al-Hasani, S. Cushman, E Eisenberg, and L. Greene Expression of auxilin or AP180 inhibits endocytosis by mislocalizing clathrin: evidence for formation of nascent pits containing AP1 or AP2 but not clathrin J. Cell Sci., January 1, 2001; 114(2): 353 - 365. [Abstract] [PDF]
|
|
|
|
|
|
A. W. Kao, C. Yang, and J. E. Pessin Functional comparison of the role of dynamin 2 splice variants on GLUT-4 endocytosis in 3T3L1 adipocytes Am J Physiol Endocrinol Metab, May 1, 2000; 278(5): E825 - E831. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kanzaki, R. T. Watson, N. O. Artemyev, and J. E. Pessin The Trimeric GTP-binding Protein (Gq/G11) alpha Subunit Is Required for Insulin-stimulated GLUT4 Translocation in 3T3L1 Adipocytes J. Biol. Chem., March 15, 2000; 275(10): 7167 - 7175. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. L. Parker and C. R. Parrish Cellular Uptake and Infection by Canine Parvovirus Involves Rapid Dynamin-Regulated Clathrin-Mediated Endocytosis, Followed by Slower Intracellular Trafficking J. Virol., February 15, 2000; 74(4): 1919 - 1930. [Abstract] [Full Text]
|
|
|
|
|
|
L. A. Garza and M. J. Birnbaum Insulin-responsive Aminopeptidase Trafficking in 3T3-L1 Adipocytes J. Biol. Chem., January 28, 2000; 275(4): 2560 - 2567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Knight, K. T. Cao, G. V. Gibson, and A. L. Olson Expression of a Prenylation-Deficient Rab4 Interferes with Propagation of Insulin Signaling through Insulin Receptor Substrate-1 Endocrinology, January 1, 2000; 141(1): 208 - 218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Lee, J. Ryu, R. P. Souto, P. F. Pilch, and C. Y. Jung Separation and Partial Characterization of Three Distinct Intracellular GLUT4 Compartments in Rat Adipocytes. SUBCELLULAR FRACTIONATION WITHOUT HOMOGENIZATION J. Biol. Chem., December 31, 1999; 274(53): 37755 - 37762. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Pessin, D. C. Thurmond, J. S. Elmendorf, K. J. Coker, and S. Okada Molecular Basis of Insulin-stimulated GLUT4 Vesicle Trafficking. LOCATION! LOCATION! LOCATION! J. Biol. Chem., January 29, 1999; 274(5): 2593 - 2596. [Full Text] [PDF]
|
|
|
|
|
|
D. Li, V. K. Randhawa, N. Patel, M. Hayashi, and A. Klip Hyperosmolarity Reduces GLUT4 Endocytosis and Increases Its Exocytosis from a VAMP2-independent Pool in L6 Muscle Cells J. Biol. Chem., June 15, 2001; 276(25): 22883 - 22891. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Huang, T. Imamura, and J. M. Olefsky Insulin can regulate GLUT4 internalization by signaling to Rab5 and the motor protein dynein PNAS, November 6, 2001; 98(23): 13084 - 13089. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
