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J Biol Chem, Vol. 273, Issue 28, 17553-17558, July 10, 1998
From the Department of Biological Chemistry, Weizmann Institute of
Science, Rehovot 71600, Israel
A 150-kDa transferrin-like protein (Ttf) is
associated with the plasma membrane of the halotolerant unicellular
alga Dunaliella salina (Fisher, M., Gokhman, I., Pick, U.,
and Zamir, A. (1997) J. Biol. Chem. 272, 1565-1570).
The Ttf level rises with medium salinity or upon iron depletion.
Evidence that Ttf is involved in iron uptake by Dunaliella
is presented here. Algal iron uptake exhibits characteristics
resembling those of animal transferrins: high specificity and affinity
for Fe3+ ions, strict dependence on carbonate/bicarbonate
ions, and very low activity in acidic pH. Reducing the level of Ttf by
mild proteolysis of whole cells is accompanied by lowered uptake
activity. Conversely, accumulation of high levels of Ttf is correlated
with an enhancement of iron uptake. Kinetically, iron uptake consists
of two steps: an energy-independent binding of iron to the cell surface
and an energy-dependent internalization. Salinities as high
as 3.5 M NaCl do not inhibit iron uptake or decrease the
apparent affinity for Fe3+ ions, implying that Ttf activity
is not affected by high salt. These results indicate that transferrins,
hitherto identified only in animals, are present and function in iron
transport also in plant systems.
Iron Uptake by the Halotolerant Alga Dunaliella Is
Mediated by a Plasma Membrane Transferrin
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
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