HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Furukawa, T. Articles by Yoshii, M. Search for Related Content PubMed PubMed Citation Articles by Furukawa, T. Articles by Yoshii, M.

J Biol Chem, Vol. 273, Issue 28, 17585-17594, July 10, 1998

Differential Interactions of the C terminus and the Cytoplasmic I-II Loop of Neuronal Ca²⁺ Channels with G-protein  and Subunits
I. MOLECULAR DETERMINATION

Taiji Furukawa^§, Toshihide Nukada, Yasuo Mori^**, Minoru Wakamori^**, Yoshihiko Fujita, Hiroyuki Ishida, Kazuhiko Fukuda^§§, Shigehisa Kato^§§, and Mitsunobu Yoshii^¶¶

From the  Department of Neurochemistry and ^¶¶ Department of Neurophysiology, Tokyo Institute of Psychiatry, 2-1-8 Kamikitazawa, Setagaya-ku, Tokyo 156, the ^§ Department of Internal Medicine, Faculty of Medicine, Teikyo University, 2-11-1 Kaga, Itabashi-ku, Tokyo 173, the  Department of Information Physiology, National Institute for Physiological Sciences, Okazaki, Aichi 444, Japan, the ^** Institute of Molecular Pharmacology and Biophysics, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0828, and the  Department of Molecular Genetics, Kyoto University Faculty of Medicine, Yoshidakonoe-cho and ^§§ Department of Anesthesia, Kyoto University Hospital, 54 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-01, Japan

Interactions of G-protein  (G) and subunits (G) with N- (_1B) and P/Q-type (_1A) Ca²⁺ channels were investigated using the Xenopus oocyte expression system. G_i3 was found to inhibit both N- and P/Q-type channels by receptor agonists, whereas G₁₂ was responsible for prepulse facilitation of N-type channels. L-type channels (_1C) were not regulated by G or G. For N-type, prepulse facilitation mediated via G was impaired when the cytoplasmic I-II loop (loop 1) was deleted or replaced with the _1C loop 1. G-mediated inhibitions were also impaired by substitution of the _1C loop 1, but only when the C terminus was deleted. For P/Q-type, by contrast, deletion of the C terminus alone diminished G-mediated inhibition. Moreover, a chimera of L-type with the _1B loop 1 gained G-dependent facilitation, whereas an L-type chimera with the N- or P/Q-type C terminus gained G-mediated inhibition. These findings provide evidence that loop 1 of N-type channels is a regulatory site for G and the C termini of P/Q- and N-types for G.

---

Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.

This article has been cited by other articles:

				B. Li, H. Zhong, T. Scheuer, and W. A. Catterall Functional Role of a C-Terminal G{beta}{gamma}-Binding Domain of Cav2.2 Channels Mol. Pharmacol., September 1, 2004; 66(3): 761 - 769. [Abstract] [Full Text] [PDF]

				A. C. Dolphin G Protein Modulation of Voltage-Gated Calcium Channels Pharmacol. Rev., December 1, 2003; 55(4): 607 - 627. [Abstract] [Full Text] [PDF]

				H. L. Agler, J. Evans, H. M. Colecraft, and D. T. Yue Custom Distinctions in the Interaction of G-protein {beta} Subunits with N-type (CaV2.2) Versus P/Q-type (CaV2.1) Calcium Channels J. Gen. Physiol., May 27, 2003; 121(6): 495 - 510. [Abstract] [Full Text] [PDF]

				E. S. Piedras-Renteria, K. Watase, N. Harata, O. Zhuchenko, H. Y. Zoghbi, C. C. Lee, and R. W. Tsien Increased Expression of alpha 1A Ca2+ Channel Currents Arising from Expanded Trinucleotide Repeats in Spinocerebellar Ataxia Type 6 J. Neurosci., December 1, 2001; 21(23): 9185 - 9193. [Abstract] [Full Text] [PDF]

				A. A. Simen, C. C. Lee, B. B. Simen, V. P. Bindokas, and R. J. Miller The C Terminus of the Ca Channel {alpha}1B Subunit Mediates Selective Inhibition by G-Protein-Coupled Receptors J. Neurosci., October 1, 2001; 21(19): 7587 - 7597. [Abstract] [Full Text] [PDF]

				H. Zhong, B. Li, T. Scheuer, and W. A. Catterall Control of gating mode by a single amino acid residue in transmembrane segment IS3 of the N-type Ca2+ channel PNAS, April 10, 2001; 98(8): 4705 - 4709. [Abstract] [Full Text] [PDF]

				H. M. Colecraft, D. L. Brody, and D. T. Yue G-Protein Inhibition of N- and P/Q-Type Calcium Channels: Distinctive Elementary Mechanisms and Their Functional Impact J. Neurosci., February 15, 2001; 21(4): 1137 - 1147. [Abstract] [Full Text] [PDF]

				S. Restituito, R. M. Thompson, J. Eliet, R. S. Raike, M. Riedl, P. Charnet, and C. M. Gomez The Polyglutamine Expansion in Spinocerebellar Ataxia Type 6 Causes a beta Subunit-Specific Enhanced Activation of P/Q-Type Calcium Channels in Xenopus Oocytes J. Neurosci., September 1, 2000; 20(17): 6394 - 6403. [Abstract] [Full Text] [PDF]

				A. A. Simen and R. J. Miller Involvement of Regions in Domain I in the Opioid Receptor Sensitivity of alpha 1B Ca2+ Channels Mol. Pharmacol., May 1, 2000; 57(5): 1064 - 1074. [Abstract] [Full Text]

				G. J. Greif, D. L. Sodickson, B. P. Bean, E. J. Neer, and U. Mende Altered Regulation of Potassium and Calcium Channels by GABAB and Adenosine Receptors in Hippocampal Neurons From Mice Lacking Galpha o J Neurophysiol, February 1, 2000; 83(2): 1010 - 1018. [Abstract] [Full Text] [PDF]

				T. Furukawa, T. Yamakawa, T. Midera, T. Sagawa, Y. Mori, and T. Nukada Selectivities of Dihydropyridine Derivatives in Blocking Ca2+ Channel Subtypes Expressed in Xenopus Oocytes J. Pharmacol. Exp. Ther., November 1, 1999; 291(2): 464 - 473. [Abstract] [Full Text]

				C. Canti, K. M. Page, G. J. Stephens, and A. C. Dolphin Identification of Residues in the N Terminus of alpha 1B Critical for Inhibition of the Voltage-Dependent Calcium Channel by Gbeta gamma J. Neurosci., August 15, 1999; 19(16): 6855 - 6864. [Abstract] [Full Text] [PDF]

				T. Furukawa, R. Miura, Y. Mori, M. Strobeck, K. Suzuki, Y. Ogihara, T. Asano, R. Morishita, M. Hashii, H. Higashida, et al. Differential Interactions of the C terminus and the Cytoplasmic I-II Loop of Neuronal Ca2+ Channels with G-protein alpha  and beta gamma Subunits. II. EVIDENCE FOR DIRECT BINDING J. Biol. Chem., July 10, 1998; 273(28): 17595 - 17603. [Abstract] [Full Text] [PDF]

				T. Ivanina, Y. Blumenstein, E. Shistik, R. Barzilai, and N. Dascal Modulation of L-type Ca2+ Channels by Gbeta gamma and Calmodulin via Interactions with N and C Termini of alpha 1C J. Biol. Chem., December 15, 2000; 275(51): 39846 - 39854. [Abstract] [Full Text] [PDF]

				M. Kinoshita, T. Nukada, T. Asano, Y. Mori, A. Akaike, M. Satoh, and S. Kaneko Binding of Galpha o N Terminus Is Responsible for the Voltage-resistant Inhibition of alpha 1A (P/Q-type, Cav2.1) Ca2+ Channels J. Biol. Chem., July 27, 2001; 276(31): 28731 - 28738. [Abstract] [Full Text] [PDF]