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J Biol Chem, Vol. 273, Issue 29, 17991-17994, July 17, 1998
From the Department of Molecular Genetics and Microbiology, State
University of New York at Stony Brook,
Stony Brook, New York 11794-5222
The Rac GTP-binding protein controls signal
transduction pathways that are critical for mitogenesis and oncogenesis
(, ). The biochemical nature of these signaling pathways is presently unknown. Here we report that a region in Rac1 (residues 124-135), previously defined as the insert region (), is essential for its
mitogenic activity. Deletion of this region does not interfere with the
ability of Rac1 to induce cytoskeletal changes or to activate the Jun
kinase mitogen-activated protein kinase cascade but abrogates
Rac1-induced stimulation of DNA synthesis and Rac1-mediated superoxide
production in quiescent fibroblasts. Treatment of cells with agents
that abolish superoxide generation inhibits specifically the mitogenic
effect of Rac1. Our results identify an effector site in Rac1 that is
necessary for mitogenic signaling and implicate superoxide generation
as a candidate effector pathway of Rac1-dependent cell
growth.
COMMUNICATION
A Rac1 Effector Site Controlling Mitogenesis through Superoxide
Production
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
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