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J Biol Chem, Vol. 273, Issue 29, 18481-18489, July 17, 1998
From the Department of Molecular Genetics, University of Texas
M. D. Anderson Cancer Center, Houston, Texas 77030
We describe the characterization of a novel gene,
shk2, encoding a second p21cdc42/rac-activated
protein kinase (PAK) homolog in fission yeast. Like other known PAKs,
Shk2 binds to Cdc42 in vivo and in vitro. While overexpression of either shk2 or cdc42 alone
does not impair growth of wild type fission yeast cells,
cooverexpression of the two genes is toxic and leads to highly aberrant
cell morphology, providing evidence for functional interaction between
Cdc42 and Shk2 proteins in vivo. Fission yeast
shk2 null mutants are viable and exhibit no obvious
phenotypic defects. Overexpression of shk2 restores viability and normal morphology but not full mating competence to
fission yeast cells carrying a shk1 null mutation.
Additional genetic data suggest that Shk2, like Cdc42 and Shk1,
participates in Ras-dependent morphological control and
mating response pathways in fission yeast. We also show that
overexpression of byr2, a gene encoding a Ste11/MAPK kinase
kinase homolog, suppresses the mating defect of cells partially
defective for Shk1 function, providing evidence of a link between PAKs
and mitogen-activated protein kinase signaling in fission yeast. Taken
together, our results suggest that Shk2 is partially overlapping in
function with Shk1, with Shk1 being the dominant protein in
function.
Cloning and Characterization of shk2, a Gene Encoding
a Novel p21-activated Protein Kinase from Fission Yeast
Copyright © 1998 by The American Society for Biochemistry and Molecular Biology, Inc.
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